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GM-CSF had dual effects on the cell growth,it could inhibit the fibroblasts proliferation at concentration abov e 1mg/L while stimulate the cell growth at concentration between 1mg/L and 0 .001mg/L.

GM-CSF的浓度在0.001~1mg/L之间对牙周膜成纤维细胞、牙髓成纤维细胞有明显的增殖效应,大于1mg/L时对增殖有明显的抑制作用。

The results showed that transformed buds could be obtained after the epicotyl segments were cultured on co-culture medium (MS+3mg/L BA) for 3 days and then on selective medium with 100 mg/L Kanamycin and 500 mg/L Cefotaxime for 2 weeks. The transformation efficency of Sweet orange with the chit42 and phyB genes reached 27.8% and 35.6%, respectively.

上胚轴经农杆菌工程菌液浸染后,在共培培养基(MS+3mg/LBA)上培养3d,然后分别转移到含有100mg/L Kan(卡那霉素,Kanamycin)和500mg/L Cef(头孢噻肟霉素,Cefotaxime)的选择培养基上培养14d后,上胚轴开始分化抗性芽,chit42基因和phyB基因的抗性芽再生率分别为27.8%和35.6%。

It is well-known that for f with compact support in [66] Stein proved that: M G ∈~L~1 for any measurable set E of finite measure if and only if f ∈ L ln~+ L.

当f有紧支集时,关于它的极大函数的可积性Stein在[66]有个著名的结果:对任意有限测度集E,M∈L~1当且仅当f∈Lln~+L。

In [1] and [35] Bagby and Gomez independently proved that there are many functions f ∈ L ln~+ L(R~2) such that M_s ∈ L~1 for any measurable set E of finite measure.

在[1]和[35]中他们分别证明了存在许多函数f∈Lln~+L(R~2)使得对任意有限测度集E,M_S∈L~1。

The result show that particle numbers of diameter of floccule which are of narrow-dispersed size between 7 500~20 000 nm are more than 82% of the total when the pH is between 8.5 and 10,with concentation of Fe~(3+) around 40 mg/L and areration rate in the range of 0.10 L/min and 0.14 L/min.

结果显示,在絮凝剂中Fe3+浓度为40 mg/L,废水的pH值为8.5~10,曝气速率在0.10 L/min~0.14L/min之间时,絮凝体颗粒粒径较大而且分布比较集中,优势粒径范围为7 500~20 000 nm,该粒径范围的絮凝颗粒占总体份额大于82%。

According to several years practice, comprehensively sum up measures of forcing culture technology of scale and dwarf on potted Bougainvillea as: a propagation of cuttage, which choose a mixture of 50%peat, 33% vermiculite and 17 sharp sand as cutting soil, and choose a semi-wooden shoot cut into 5~10cm length with 2~3 leaves as cuttings then touch 1% AA root powder, is lower cost and higher survival rate than that of tissue culture; Bougainvillea growth can be efficaciously regulated by pruning and using 2~5g/L PP333; bougainvillea can be induced to flowers by spraying 2.5~4.0g/L B9 and administering no more 11 hours daylight every day continuously; the florescence can be more prolonged about 20 days by spraying 50mg/L NAA while selling; and prevention and cure of pest and disease, etc.

根据多年的生产实践,从科学育苗、栽培管理、售前处理和病虫害防治等方面综合总结了叶子花的规模化矮化促成生产技术措施:选择泥炭土、蛭石和黄沙按3:2:1比例混合均匀的扦插基质,采用半木质化当年生枝条,剪成5~10cm长的2~3叶节一段,沾染1%自制生根粉的扦插育苗较组培育苗成本低,成活率高;结合修剪用2~5g/L的多效唑进行叶面喷洒能有效控制生长,矮化植株;选择2.5~4.0的丁酰肼叶面喷洒和每天保持不多于11h的短日照处理,能有效促进开花;销售前全株喷洒脱50mg/L NAA可延长花期20天左右;整个生产过程加强肥水管理、温湿度控制和环境清洁能有效防治病虫害发生。

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2~0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%~80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0~0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法,将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合,建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺:胰酶用pH4.5醋酸缓冲溶液提取后,粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液:0.01mol/LNaoAc-HoAc缓冲液(pH4.5,洗脱缓冲液:0.01mol/LNaoAc-HoAc缓冲液(pH4.5)含0.2~0.35mol/LNaCl分两步洗脱,收集激肽释放酶的洗脱峰;DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液:0.01mol/LTris-HCl缓冲液(pH7.5,洗脱液:0.5mol/LNaCl溶液,一次洗脱,收集胰蛋白酶洗脱峰;最后,亲和层析未吸附液用30%~80%硫酸铵分级盐析处理,沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液:0.01mol/LNaAc-HAc缓冲液(pH4.5含1mol/L(NH_4)_2SO_4,洗脱缓冲液:0.01mol/LNaAc-HAc缓冲液(pH4.5)含0~0.6mol/L(NH_4)_2SO_4,分两步洗脱,收集糜蛋白酶的洗脱峰。

On the basic that existing second-order sufficient condition for an isolated minimizer of order 2 for unconstrained multi-objective optimization, using the so called oriented distance from a point to a set, l-stable function and generalized second-order Peano direction derivative, some second-order sufficient conditions for an isolated minimizer of order 2 for constrained multiobjective optimization problems with l-stable functions are obtained.

在已有的无约束l-稳定函数多目标优化问题的二阶充分条件的基础上,借助定向距离函数和l-稳定函数的性质及引入的广义二阶Peano方向导数,进一步刻画了具有抽象约束的l-稳定函数的多目标优化问题的二阶孤立极小点的二阶充分条件。

RESULTS: The total GKB yields were enhanced 69%, 13.8%and 11.4%compared with the control by adding 100 mg.L-1 of isoprene and low concentrations (10 and 50 mg.L-1) of geraniol in the medium, respectively.

结果:于培养基中添加100 mg.L-1异戊二烯及低浓度(10 mg.L-1,50 mg.L-1)的牛儿醇能提高GKB的产量,分别比对照增加了69%,13.8%和11.4%。

The results indicated that the inhibited concentrations of kanamycin of adventitious bud formation of Helianthus annus were 75 mg/L and 100 mg/L, when the carbenicillin was used in the transformation procedure; the concentrations was 300 mg/L when the growth of agrobacterium could be effectively inhibited. At the same time, the induction and growth of adventitious bud were not remarkably injured.

在以卡那霉素为抗性选择标记时,对油葵不定芽起完全抑制作用的Km临界浓度在75~100 mg/L;在以羧节青霉素为抑菌剂用于油葵遗传转化试验时,其浓度为300 mg/L时即可有效抑制农杆菌的生长,同时又不会对不定芽的诱导及生长产生明显的伤害作用。

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推荐网络例句

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。