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The MTT assay was used to detect the viability of cells. Flow cytometry and laser confocal microscopy were used to identiyfy the cell cycle and apoptotic morphology after cells treated with LAO. RESULTS: An L-amino acid oxidase was successfully purified from the venom of bungarus fasciatus.

结果： 通过两步层析法从金环蛇毒中分离得到L-氨基酸氧化酶，L－氨基酸氧化酶的表观分子量通过SDS-PAGE测定约为60 kD，通过凝胶过滤测定分子量为70 kD.L－氨基酸氧化酶作用于HUVCE细胞12 h后，细胞的生长明显受到抑制，L－氨基酸氧化酶作用的半数抑制量为2.8 mg/L。

The effects of different nutritional condition on the growth and antioxidant enzyme activity of submerged macrophytes Myriophyllum spicatum L, Hydrilla verticillata L and Ceratophyllum demersum L. were investigated in this study.

模拟研究了沉水植物狐尾藻Myriophyllum spicatum L、轮叶黑藻Hydrilla verticillata L和金鱼藻Ceratophyllum demersum L在中、富两种营养水平下的生长及生理特性。

PKC and PKA activity assays showed that 0.03μmol/L of 20-HETE increased PKC and PKA activity in isolated cardiomyocytes, while chelerythrine (5μmol/L) and H-89 (5μmol/L) inhibited these effects.

PKC和PKA活性测定的结果表明20-HETE(0.03μmol/L)增加心肌细胞PKC和PKA的活性，并且白屈菜赤碱(5μmol/L)和H-89(5μmol/L)抑制该效应。

Conclusion］ By comprehensive comparison,the fresh-keeping effect of 1% Sugar+200 mg/L 8-HQS+ 25 mg/L AgNO3 +50 mg/L Al2(SO4)3 is best,its vase life reaches 22 days,which is 10 and 3 d longer than control and chrysal,respectively; moreover,it can nicely keep leaf color and prevent petals falling.

结论］综合比较，以1%蔗糖+200 mg/L 8-HQS +25 mg/L AgNO3 +50 mg/L Al2(SO4)3保鲜效果最佳，瓶插期达22 d，分别比对照和可利鲜延长了10和3 d；而且能较好地保持其叶色并不掉花瓣。

The results showed that attached Cladophora oligoclona grew quite well and had high removal rates of N and P in the eutrophic lake water at the concentrations of TN 10.512mg/L, NH4-N 8.563mg/L, TP 0.856mg/L and PO4-P 0.573mg/L.

结果显示，刚毛藻在原始浓度总氮10.512mg/L和总磷0.856mg/L的富营养化湖水中能维持正常的生长代谢，并能有效去除水体中的N、P养分。

Sorghum, sudenese grass and orchard-grass (Dactylis glomerata L.), and the application of MeJA for the flowering synchronization of male sterile and restorer lines was investigated in seed production of hybrid rice.

外源MeJA强烈诱导稻、高粱、苏丹草和果园草等禾本科植物的颖花开放：早稻297的离体穗在经40μmol/L，400μmol/L和4mmol/L的MeJA溶液浸穗 2min处理后80min，在对照颖花开放尚未启动时，平均每穗颖花开放率分别达 7.5％，22.7％和44.5％；杂交稻安湘S-株173的连穗经4mmol/L的MeJA溶液浸穗2min处理后80min，开颖率达32.5％；不育系也能响应MeJA的诱导，经1mmol/L MeJA处理后，80min时开颖率达42.9±6.1％。

The results indicated the best medium for supension co-culture with Daucus carota and Daucus carota is MS, 2, 4-D 2 mg/L, NAA 1 mg/L and KT 0.2 mg/L, after 25 d the polysaccharide content of co-culture reaches the highest level 0.203 mg/g, and the saponin content of co-culture is 1.85mg/g, higher 3.35% than saponin of Panax ginseng culture.

结果表明，人参与胡萝卜悬浮混合培养的最佳基质为MS、2，4-D2mg/L、NAA1mg/L、KT0.2mg/L，在培养25d时混合培养物的多糖含量达到最高，浓度达到0.203mg/g，混合培养物的皂苷含量为1.85mg/g，比人参悬浮培养物皂苷含量高出3.35%。

Result: At most up to 500 mg·L-1, tetrahydropalmatine showed no sinificant effect on the cell viability, while dehydrocorydaline, berberine and palmatine significantly decreased the cell viability, exceeding 6.3, 0.6 and 6.3 mg·L-1, respectively（ P .05 or P .01）. Tetrahydropalmatine, dehydrocorydaline, berberine and palmatine significantly inhibited LDH leakage induced by anoxia-reoxia injury, at concentrations of 50-100, 1.25-5, 4 and 30 mg·L-1, respectively（ P .05 or P .01）. None of the four compounds showed significant effect on the hydrogen peroxide injury.

结果：多至500 mg·L-1的延胡索乙素对细胞活力无任何影响，脱氢紫堇碱、黄连素、巴马汀分别在质量浓度大于6.3， 0.6，6.3 mg·L-1时，显著降低细胞活力（ P 。05， P 。01）；延胡索乙素、脱氢紫堇碱、黄连素和巴马汀分别在质量浓度为50～100， 1.25～5， 4，30 mg·L-1时，显著抑制缺氧复氧导致的LDH漏出（ P 。05， P 。01）；4种单体对过氧化氢损伤无显著的保护作用。

Result: At most up to 500 mg·L-1, tetrahydropalmatine showed no sinificant effect on the cell viability; while dehydrocorydaline, berberine and palmatine significantly decreased the cell viability, exceeding 6.3, 0.6 and 6.3 mg·L-1, respectively（ P .05 or 0.01）. Tetrahydropalmatine, dehydrocorydaline, berberine and palmatine significantly inhibited LDH leakage induced by anoxia-reoxia injury, at concentrations of 50-100, 1.25-5, 4 and 30 mg·L-1, respectively（ P .05 or 0.01）. None of the four compounds showed significant effect on the hydrogen peroxide injury.

结果：多至500 mg·L-1的延胡索乙素对细胞活力无任何影响，脱氢紫堇碱、黄连素、巴马汀分别在质量浓度大于6.3， 0.6，6.3 mg·L-1时，显著降低细胞活力（ P 。05或0.01）；延胡索乙素、脱氢紫堇碱、黄连素和巴马汀分别在质量浓度为50～100， 1.25～5， 4，30 mg·L-1时，显著抑制缺氧复氧导致的LDH漏出（ P 。05或0.01）；4种单体对过氧化氢损伤无显著的作用。

The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.

体外培养心肌细胞，用100 TCID50柯萨奇B3病毒（CVB3）感染心肌细胞，建立病毒性心肌炎实验模型，然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞，观察心肌细胞的形态和搏动，用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白，四唑蓝比色法测定心肌细胞活性和病毒抑制率，流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。