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The culture medium is composed of human keratinocyte serum free culture medium +2.5 mg/L bovine pituitary lixivium +5 μg/L epidermal growth factor+438 mg/L glutamine, glutamine can promote the growth of the keratinocyte.

采用改良的无血清培养技术进行人体角质形成细胞的培养,培养基为人角质形成细胞无血清培养基+2.5 mg/L牛垂体浸出液+5 μg/L表皮生长因子+438 mg/L谷氨酰胺,其中谷氨酰胺能促进角质形成细胞的生长。

Methods The cells were divided into 5 treatment groups(10,25,50,75 and 100 μmol·L-1 QUE), blank control and menstruum control group. The rat C6 cells were cultivated to 1×106·mL-1 in the RPMI 1640 medium, then added into 96 holes board with various doses of QUE by 3 holes per group,and MTT assay was used to observe the proliferation of the cells treated for 24,48 and 72 h. The change of cell cycle was also observed by flow cytometry after the cells were treated with 50 and 100 μmol·L-1 QUE for 48 h. The changes of the protein P53 and Bcl-2 of C6 cells treated with 50 μmol·L-1 QUE for 48 h were detected by immunocytochemical methods.

按QUE浓度分成10、25、50、75及100 μmol·L-15个处理组和空白对照组及溶剂对照组,大鼠脑胶质瘤C6细胞在RPMI 1640培养基中生长达1×106·mL-1后,在96孔板中分别加入上述浓度的QUE继续培养,每组设3复孔,作用24、48及72 h,采用MTT比色法检测QUE对大鼠脑胶质瘤C6细胞的增殖抑制情况,流式细胞术对50及100 μmol·L-1的QUE作用48 h的大鼠脑胶质瘤C6细胞进行周期分析,免疫组化法检测50 μmol·L-1的QUE作用48 h 的p53和bcl-2基因产物。

In order to observe the effects of mercuric chloride to physiological and biochemical indexes of blood at low doses in SD rats, 24 SD rats were separated into control group, low dose group of mercury (50 mg/L), middle dose group of mercury (100 mg/L), high dose group of mercury (200 mg/L), which were given in the drinking water.

为了研究低剂量汞对SD大鼠血液生理生化指标的影响,24只SD大鼠被随机分为4组,分别为对照组,汞低剂量组(50 mg/L),汞中剂量组(100 mg/L),汞高剂量组(200 mg/L),采用自由饮水染毒,染毒时间为21d。

To explore the issue, 16 experiments to dissolve microcline have been conducted using different solutions with 2mol/L NaCl, 0.05mol/L acetic acid, 5ml crude oil or 0.05mol/L oxalic acid (the values are close to those measured in Tarim oilfield waters) and different initial pHs and temperatures.

为探讨该问题,实验配制了含2mol/L NaCl 、0.05mol/L 乙酸、5ml 原油、0.05mol/L 草酸(这些值接近塔里木盆地油田水实测值),并控制初始 pH 0、温度,进行了16种不同条件下长石溶解作用的模拟实验研究。

When the structure consists 50 per PO, The oleaginousness of waste water from recovery liquid about polymer flooding will descend from 50000mg/l to 300mg/l after adding 40mg/l LSEPE solution. The product of LSEPE has the property of demulsifier and electric dehydration.

结果表明,该产物是有效的聚合物驱采出液的处理剂,当结构中PO量占50%时,添加40mg/l的LSEPE可使注聚采出液分离的污水含油从50000mg/l左右降到300mg/l,兼具脱水和脱盐性能。

Distribution of cells along phases of the cell cycle was analyzed by flow cytometry. Results Glucose 15 or 30 mmol/L, palmitate 0.25 or 0.5 mmol/L, and oleate 0 .5 mmol/L inhibited proliferation and accelerated death of endothelial cells in a dose-and-time-dependent manner.

结果 15或30 mmol/L 的葡萄糖、0.25或0.5 mmol/L的棕榈酸,以及0.5 mmol/L的油酸对内皮细胞产生浓度-效应和时间-效应的增殖抑制作用和加速细胞死亡的作用。

In Chapter four, the stability problem of the nonlinear open-loop control system is discussed by the concept of input-output stability. The L〓 stability is defined, the relationship between the L〓 norm of output variable and the input variable is proven firstly, and the sufficient condition for the stability of open-loop system , the H〓 norm of the n-th order frequency response function decreasing by n!

本章给出了非线性控制系统L〓稳定性的定义,首次严格证明了输出变量的L〓范数与输入变量的L〓范数之间的一个重要的关系式,并得到了一个开环系统L〓稳定性的充分条件,即n阶广义频率响应函数的范数〓应按n!

Results: The proliferation of Kasumi-l cell was obviously inhibited by VPA on dose- and time-dependent manner. The IC50 was 2.33 mmol/L treated with VPA for 72 h. Incubation with VPA, the cell ratio at G0/C1 phase increased. VPA induced myeloid cell differentiation antigen CD11b to increase with a time-and dose-dependent way and caused apoptosis. Wright's staining and light microscope were used to show the morphology of partial differentiation and obvious apoptosis with 3 mmol/L VPA for 72 hours. The typical DNA ladder of apoptosis characteristic was observed with DNA gel electrophoresis.

结果:VPA能显著抑制Kasumi-1细胞的增殖,且呈剂量和时间依赖性,VPA处理Kasumi-1细胞72h时的半数抑制浓度(IC50)为2.33 mmol/L;VPA处理后,细胞周期检测G0/G1期细胞比例逐渐增高;VPA能诱导髓系分化抗原CD11b表达水平的阳性率升高,呈时间及剂量依赖性;VPA能诱导Kasumi-1细胞凋亡,Kasumi-l细胞经VPA3 mmol/L处理72h后,出现典型的凋亡细胞所具有的梯形DNA条带。

The results showed that 2,4 D had promotive effects on the growth of pavlova viridis, especially in the early stage of culture, and the optimum mass concentration was 0.1 mg/L. 6 BA had lethal effect on pavlova viridis, and the lowest lethal mass concentration was 1.0 mg/ L.

结果表明,在试验浓度范围内,2 ,4— D对绿色巴夫藻生长的影响在培养早期比后期明显,最适浓度为 0 。1 m g/L ;6-BA质量浓度≥ 1 mg/L时对绿色巴夫藻有致死作用,质量浓度为 0 。1~ 0 。8mg/L时,对绿色巴夫藻的生长有促进作用,促进生长的最适质量浓度为 0 。5mg/L。

Quality wild grape wine with 11.6% alcohol content, total acid 8.5g/L, residual sugar 3.6g/L, and steeping material 27.5g/L was produced with full-black wild grape over 90% the amount of the raw materials and dry white wine, imported wine yeast, sugar, pectase and albumen powder as its auxilliary materials by techniques of fermentation and sprinkle etc.

摘 要:选用90%以上的全黑山葡萄为原料,干白原酒、进口葡萄酒酵母、白砂糖、果胶酶、蛋白粉为辅料,经发酵、淋酒等酿造工艺可生产出11.6度、总酸8.5g/L、残糖3.6g/L、浸出物为27.5g/L的优质山葡萄酒。

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Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

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However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。