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In order to investigate the relationship between the open-chain ligands and metal ions, and the possibility of the metal complexes used as electroluminescent materials, a new naphthol schiff base ligand which is a open-chain crown and its four transition metal complexes, a new amide type tripodal ligand containing heterocyclic ring, two tripodal ligands (H〓L〓 and H〓L〓) containing phenol schiff bases and their rare earth complexes were synthesized and characterized.

为了进一步研究和探讨开链配体与金属离子的配位作用，以及金属配合物作为电致发光材料的可能性，我们设计合成了含萘酚环的Schiff碱开链冠醚H〓L〓及四种过渡金属(Zn、Ni、Co、Cu)配合物，两个含酚羟基的Schiff碱三足配体H〓L〓和H〓L〓及稀土配合物，一个含杂环的酰胺型三足配体L〓，培养了配体L〓的单晶，确定了其晶体结构。

N - Fmoc - L - B - homoglutamine and N - Fmoc - L - B - homoasparagine have been prepared by anidt - eistert synthesis from commercially available N - Fmoc - L - a - glutamine and Na - Fmoc - Nr - trityl - L -asparagine.

描述了应用Arndt-Eistert反应合成两种含酰胺基的N-Fmoc-L-β-氨基酸的方法，讨论了在Wolff重排过程中，N-Fmoc-L-α-谷氨酰胺重氮甲基酮和N-Fmoc-L-α-天冬酰胺重氮甲基酮的各自特点及反应过程中生成的副产物。

The removal efficiency of L-acid and TOC of solution is 57% and 53% respectively when 0.4 g doped photocatalyst is applied in 100 mg·L-1 L-acid solution illuminated by visible light for 4 hours, while it is 13% and 10% respectively in the case of the undoped photocatalyst.

Mo6+的掺杂不影响负载催化剂的紫外光活性及对L-酸的吸附能力。100 mg·L-1的L-酸溶液加0.4 g催化剂，在可见光下反应4 h，掺杂催化剂对L-酸和溶液总有机碳的去除率分别为57%和53%，而未掺杂的催化剂去除率分别为13%和10%。

The vasomotion was induced by noradrenaline in vivo, then the changes of vasomotion after injecting SI and Na-monomethyl-L-arginine （L-NMMA, a NO synthase inhibitor） were measured respectively on a TV monitor using a TV camera mounted on the microscope, and the influence of L-NMMA on effect of SI was also observed.

在活体情况下运用去甲肾上腺素（noradrenaline，NA）血管运动诱导法诱导出血管运动，分别观察静脉注射N^G-单甲基-L-精氨酸（NG_monomethyl.L-arginine，L-NMMA）、SI后血管运动的变化及应用一氧化氮合酶抑制剂L-NMMA对SI作用的影响，摄录结果于监视器上用暂停功能测量。

The results show that mediums of 3/4MS+ZT0.5~1 mg/L+NAA0.01mg/L and 1/2MS+BA0.5mg/L+NAA0.01mg/L,were suitble for the geminating of shoot tips and auxiliary buds.

结果表明：诱导腋芽、顶芽萌发较理想的培养基为：3/4MS+ZT0 。5～ 1mg/L +NAA0 。0 1mg/L 和 1/2 MS+BA0 。5 mg/L+NAA0 。

Finally indicated, the best seed raise conditions of T11-1 and O7-7 is: 100mL seed culture mudium in 200 mL triangle bottle ,16 hours in shake bed;It′s best component fermentation medium is: peptone 5.0,yeast extract 3.0, beef paste 2.0,xylose 60.0,K_2HPO_4 2.0,MnSO_4·4H_2O 0.04,MgSO_4·7H_2O 0.20,FeSO_4·7H_2O 0.05;The best fermentation condition is: 150mL fermentation medium in 250mL bottle,vaccinates 10 %, 48 hours in shake bed under 46℃;The output of L-lactic acid using T11-1 and O7-7 from corncob respectively were 31.30g/L and 30.88g/L,and the conversion rate respectively were 52.17% and 51.47%.

结果表明，T11-1和O7-7的最佳种子培养条件为：在200mL三角瓶中于盛装100mL种子培养基，摇床培养16h；其最佳发酵培养基组分为：蛋白胨5.0，酵母浸粉3.0，牛肉膏2.0，木糖60.0，MnSO_4·4H_2O 0.04，MgSO_4·7H_2O 0.2，FeSO_4·7H_2O 0.05，K_2HPO_4 2.0；最佳发酵条件为：250mL输液瓶装载150mL发酵培养基，接种10%，在46℃条件下摇床发酵48h；利用T11-1和O7-7进行玉米芯L-乳酸发酵，其L-乳酸产量分别为：31.30g/L和30.88g/L，转化率分别为52.17%和51.47%。

A competitive ELISA kit for detect ENR was developed with ENR mAb and its traits were tested.Two hybridoma lines were filtered and the best one was 4G1-B3,which secreted ENR mAb with indirect ELISA titers of 1∶1.024×10~6 in ascites.Isotype of the two mAb was IgG_1.ENR mAb had a high affinity constant with 9×10~(10)L/mol.The ENR-Kit had the linear detection range of 0.05～101.6μg/L,the sensitivity of 0.05 μg/L and a good sensitivity with an IC_(50) of 1.1μg/L to ENR,cross-reactivity to other compounds less than 0.01%.The average recoveres of ENR spiked in chicken,liver,fish and milk were 81.5%,87.6%,84.3% and 95% respectively.The coefficient variation was below 10%.

结果表明：筛选出的2株杂交瘤细胞，单抗亚类均为IgG1型，其中4G1-B3株的ENR mAb间接ELISA效价为1：1.024×106，亲和常数为9×1010L/mol；竞争ELISA试剂盒的线性检测范围为0.05~101.6μg/L、灵敏度0.05μg/L、半数抑制浓度(IC50)1.1μg/L，与其他化合物的交叉反应率均小于0.01%，鸡肉样、鸡肝样、鱼肉样和牛奶样的平均添加回收率分别为81.5%8、7.6%、84.3%和95%，不同基质添加恩诺沙星标准品做竞争ELISA对ENR-Kit检测结果影响小，试剂盒保存期6个月以上。

Compared with the control, the percentages of living microorganisms on the biomembrane were 27.6, 14.1 and 0.01, respectively, after extracted by hydroxylamine hydrochloride （0.01mol·L^-1 NH2OH·HCl ＋ 0.01mol·L^-1 HNO3 ）, sodium dithionite （ 0.4mol·L^-1 Na2S2O4, pH 6.0 ）, and acidified ammonium oxalate.

经0.01mol·L^-1 NH2OH·HCl＋0.01mol·L^-1 HNO3、0.4mol·L^-1 Na2S2O4（pH6.0）和0.2mol·L^-1草酸氨（pH4.0）萃取后存活的微生物总量分别下降到原膜的27.6％、14.1％和0.01％；经15％HNO3萃取后，膜上只有极少数的细菌存活；而经H2O2／HNO3萃取后则无细菌存活、原生动物和藻类存活，说明选择性化学萃取剂的使用影响生物膜的活性。

Epicotyls were cutted longitudinally and cultured in the dark first,on the MS basal medium with 3 mg/L 6-BA and 30 g/L sugar,100% adventitious shoot induction and an average of 33.8 shoots per explant were obtained.The base of the adventitious shoots was treated with 1000 mg/ L IBA for 10 min,then placed onto the filter papers which ends dipped into liquid medium with...

上胚轴纵切结合前期暗培养，在MS附加6-BA 3mg/L、蔗糖30g/L培养基中，不定芽诱导率为100%，每个外植体平均诱导的不定芽数达33.8；不定芽在1 000mg/L IBA溶液中浸泡10min后，移入1/4MT+1/2MT+10g/L蔗糖的液体培养基中，采用滤纸桥法进行培养，生根率达100%，平均根数2.5条；移苗成活率达100%。

Methods Hepatic cells were obtained from fetal rats by means of mechanical separation and hanging-drop culture, and after two days of primary culture, the medium was changed for further cell culture in the presence of 20% fetal bovine serum (containing glucose 25ml/L, mycillin 100 U/ml, pH 7.6), 10 mmol/L nicotinamide, 1 mg/L insulin, affix N2, basic fibroblast growth factor, stem cell factor, epidermal growth factor and leukemia inhibitory factor.

采用机械分离法结合悬滴培养法获得胎肝干细胞，原代培养2d后改变培养基，添加含20%胎牛血清的DMEM（含25ml/L葡萄糖，100U/ml青链霉素，pH值7.6），添加烟酰胺10mmol/L，胰岛素1mg/L，N2添加剂，碱性成纤维细胞生长因子、表皮生长因子、干细胞因子、白血病抑制因子各20μg/L诱导分化为nestin阳性细胞。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。