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Result: The nm-HAP-sol present the dispersed and uniformity needle. A431cells inhibition ratio was maximizing at the 5 day in the 400mg/L nm-HAP-sol group, the 200mg/L nm-HAP-sol group and the 100mg/L nm-HAP-sol group, inhibition ratio of the 400mg/L nm-HAP-sol group, the positive control group and the 50mg/L nm-HAP-sol group was respectively (77.89±6.29)%,(77.46±8.26)% and (1.23±0.15)%. It was detected that the number of A431 cells were persistently increasing in the 50mg/L nm-HAP-sol groups while decreasing in the 400mg/L nm-HAP-sol group, the positive control group and abidingly increasing for 4 and 3 days then decreasing in the 100mg/L and 200mg/L nm-HAP-sol group by inverted microscope.

结果：超声乳化法配制的HAP纳米溶胶呈均匀分散的针状；400mg/L、200mg/L和100mg/L溶胶组对A431细胞抑制率在第5天达到各组最大值，此时400mg/L溶胶组与阳性对照组的抑制率分别为（77.89±6.29）%和（77.46±8.26）%， 50mg/L溶胶组抑制率为（1.23±0.15）%；倒置显微镜下见50mg/L溶胶组细胞数目持续增加，聚集成簇，细胞呈现多角形或梭形，胞浆透亮，而100mg/L 和200mg/L溶胶组的细胞数目分别增加至第4天和第3天开始下降，400mg/L溶胶组和阳性对照组细胞数目持续减少，细胞体积缩小，漂浮细胞增多。

Aldrin at concentrations higher than 40 μg/L decreased life expectancy at birth of F1 generation of the cladoceran, that at 20 μg/L and 80-640 μg/L decreased net reproduction rate, that at 320 and 640 μg/L shortened generation time, and that at all the concentrations decreased intrinsic rate of population increase. Aldrin at 160-640 μg/L shortened life expectancy at birth and generation time of F2 generation of the cladoceran, that at 160 and 640 μg/L decreased net reproduction rate, but that at all the concentrations except for 40 and 320 μg/L increased intrinsic rate of population increase.

浓度高于40μg/L的艾氏剂使多刺裸腹溞F1代的生命期望显著缩短，20μg/L和80~640μg/L的艾试剂使F1代的净生殖率显著降低，320μg/L和640μg/L的艾氏剂使F1代的世代时间显著缩短，所有浓度的艾试剂均使F1代的种群内禀增长率显著降低。160~640μg/L的艾氏剂使多刺裸腹溞F2代的生命期望和世代时间显著缩短，160μg/L和640μg/L的艾氏剂使F2代的净生殖率显著降低；除40和320μg/L外，所有浓度的艾试剂均显著提高了F2代的种群内禀增长率。

The questing for long service electroless Nickel technology leads to the preferable formula and operation conditions as follows: Nickel Sulphate 25g/L, Sodium Hypophosphate 30g/L, Lactic Acid 20~ 25ml/L, Citric Acid 5g/L, Sodium Acetate 15g/L, Thiourea l~3mg/L, Stabilizer E Img/L.

研究得出的最佳长寿命镀镍工艺为；硫酸镍25g/L，次亚磷酸钠30g/L，乳酸20ml/L，柠檬酸5g/L，乙酸钠15g/L，硫脲1～3mg/L，稳定剂E1mg／L镀液pH值5.0～5.1，施镀温度90±2℃，装载量1dm~2/L；每60min测量镀液中消耗的有效成分和补加浓缩液。

As a whole,the notable characteristic of primary productivity in size-fractioned structure was that nanoplankton occupied comparatively significant advantage in Beibu Gulf.Nanoplankton has the largest contribution to gross primary productivity,and picoplankton was the secondary contributor,while microplankton the least.The contribution of microplankton for primary productivity in the north Gulf was more than that in the other waters.Nanoplankton and picoplankton contribute more to the gross primary productivity in offshore deep waters than in inshore shallow waters3.The Beibu Gulf can be divided into three ecoregions:Region-Ⅰis the ecoregion in inshore shallow waters of the north Gulf.In average,the water depth is 18m,DIN is 1.88μmol/L,DIP is 0.20μmol/L,N:P is 9.4:1,dissolved silicate is 5.17μmol/L,the Chl a conentration in surface layer is 2.27mg·m~(-3),the assimilation index in surface layer is 3.80mg/,the primary productivity is 198.78mgC/(m~2·d), and potential fishery production is estimated to be 0.24gC/(m~2·a) according to the primary productivity.The ecoregion is mainly affected by the northern coastal water systems,and may be fit for aquaculture;Region-Ⅱis the ecoregion in offshore deep waters of the north Gulf and the coastal shallow waters to the west Hainan Island.In average,the water depth is 35m,DIN is 2.01μmol/L,DIP is 0.18μmol/L,N:P is 11.2:1,disovled silicate is 4.23μmol/L,the chlorophyll a of surface layer concentration is 1.45mg·m~(-3),the assimilation index of surface layer is 4.12 mg/,the primary productivity is 276.60mgC/(m~2·d),and the estimated potential fishery production is 0.34gC/(m~2·a) according to the primary productivity. The ecoregion was mainly influenced by ocean current from the South China Sea, rivers in the west coast of Hainan Island and the water from Qiongzhou Strait.It may be fit for aquaculture and fishery;Region-Ⅲis the ecoregion in offshore deep waters of the mid and south Gulf.In average,the water depth is 75m,DIN is 0.77μmol/L, DIP is 0.15μmol/L,N:P is 5.1:1,disovled silicate is 3.05μmol/L,the chlorophyll a of surface layer concentration is 0.70mg·m~(-3),the assimilation index of surface layer averaged is 3.69mg/,the primary productivity is 350.89mgC/(m~2·d),and the estimated potential fishery production is 0.43gC/(m~2·a) according to the primary productivity.The ecoregion was mainly affected by the circulation inside Beibu Gulf, and may be fit for fishery.

初级生产力的粒级结构的一个显著特点是总体上微型浮游生物在全调查海区均占较明显优势，对总初级生产力的平均贡献最大；微微型浮游生物对初级生产力的平均贡献次之；小型浮游生物对总初级生产力的平均贡献最小；湾北部小型浮游生物对初级生产力的平均贡献高于湾中部和湾南部，而湾中部和湾南部微型和微微型浮游生物对初级生产力的平均贡献高于湾北部，远岸深水区高于近岸浅水区。3、北部湾可以分为三个生态区：湾北部近岸浅水区，该区的环境主要特点是平均水深19m，DIN浓度平均值为1.88μmol/L，DIP浓度平均值为0.20μmol/L，N：P为9.4：1，硅酸盐浓度平均值为5.17μmol/L，表层叶绿素a平均值高达2.27 mg·m~(-3)，表层同化指数平均为3.80 mg/，初级生产力平均值198.78mgC/(m~2·d)，根据初级生产力计算潜在渔业资源碳生产量平均为0.24 gC/(m~2·a)，该区主要受湾北部沿岸水系影响，适合作为水产养殖区；湾北部深水区和海南岛西部沿岸浅水区，该区的环境主要特点是平均水深35m，DIN浓度平均值为2.01μmol/L，DIP浓度平均值为0.18μmol/L，N：P为11.2：1，硅酸盐浓度平均值为4.23μmol/L，表层叶绿素a平均值1.45 mg·m~(-3)，表层同化指数平均为4.12mg/，初级生产力平均值276.60 mgC/(m~2·d)，根据初级生产力计算潜在渔业资源碳生产量平均为0.34 gC/(m~2·a)，该区主要受南部湾口区海流向湾内延伸，沿岸海南岛河流注入湾内和琼州海峡过道水的影响，适合作为渔业作业区和水产养殖区；湾中部和南部远岸深水区，该区的环境主要特点是平均水深75m，DIN浓度平均值为0.77μmol/L，DIP浓度平均值为0.15μmol/L，N：P为5.1：1，硅酸盐浓度平均值为3.05μmol/L，表层叶绿素a平均值0.70 mg·m~(-3)，表层同化指数平均为3.69 mg/，初级生产力平均值350.89 mgC/(m~2·d)，根据初级生产力计算潜在渔业资源碳生产量平均为0.43 gC/(m~2·a)，该区主要受北部湾环流影响，适合作为渔业作业区。

The linear ranges of determination for pyrogallol, phloroglucinol, pyrocatechol, resorcinol and parodioxybenzene were 1.0×10〓～2.0 ×10〓mol l〓, 1.0×10〓～1.0×10〓mol l〓, 1.0×10〓～6.0×10〓mol l〓, 1.0 ×10〓～2.0×10〓mol l〓 and 1.0×10〓～6.0×10〓mol l〓 respectively, and their detection limits were 7.2×10〓mol l〓, 6.8×10〓mol l-1, 2.1 × 10〓mol l〓, 7.1×10〓 mol l〓 and 2.2×10〓mol l〓 respectively.

该方法测定连苯三酚、间苯三酚、邻苯二酚、间苯二酚和对苯二酚的线性范围分别为1.0×10〓～2.0×10〓mol l〓，1.0×10〓～1.0×10〓 mol l〓，1.0×10〓～6.0×10〓mol l〓，1.0×10〓～2.0×10〓mol l〓和1.0×10〓～6.0×10〓mol l〓；检出限分别为7.2×10〓mol l〓、6.8×10〓 mol l〓、2.1×10〓 mol l〓、7.1×10〓mol l〓和2.2×10〓mol l〓。

The results were showed that embryogenic calli were induced from young leaves, which were cultured on MS medium supplemented with 2,4-D 2mg/L and KT 0.2mg/L. For the proliferation of embryogenic calli, the suitable culture medium was MS+BA 8mg/L +NAA 1mg/L. The development and maturation of somatic embryo could be much improved by using the medium of MS+ZT 2mg/L or BA 5mg/L +IAA 0.2mg/L. For the induction of secondary somatic embryo from integral somatic embryo, the suitable culture medium was MS+KT 0.1mg/L+NAA 0.01 mg/L or MS+ZT 0.1mg/L+NAA 0.01 mg/L, the proliferation frequency is 214%, 256% respectively. The cotyledonary generated from somatic embryos of Aesculus hippocastanum L.

本文就欧洲七叶树的组织培养和体细胞胚发生以及植株再生进行了系统研究，主要结果如下：以植物细胞具有全能性的理论为依据，以欧洲七叶树幼嫩叶片为外植体，进行体细胞胚胎发生研究，研究结果表明，诱导愈伤组织的适宜培养基是MS+2，4-D 2mg／L+KT0.2mg／L，MS+BA 8mg／L+NAA 1mg／L有利于胚性愈伤组织的诱导和增殖，添加ZT 2mg／L或BA 5mg／L和IAA 0.2mg／L的MS培养基有利于体细胞胚发育和成熟，体细胞胚可直接诱导次生胚发生，MS+KT 0.1mg／L+NAA 0.01 mg／L或MS+ZT 0.1mg／L+NAA 0.01 mg／L培养基诱导效果最好，增殖频率分别为214％、256％。

For the bud directly differentiation , the suitable culture medium was MS+BA 2mg/L +NAA 0.2mg/L or MS+TDZ0.01mg/L +NAA0.2 mg/L, of which the induction rate was 88% and 100% respectively. An efficient tissue culture system has been developed with the bud of mature seed of Aesculus hippocastanum L. as explants . Buds were induced from 2cm high young plantlet cultured on MS medium supplemented with 0.6 mg/L 6-BA plus 0.1mg/L or 0.4~0.6mg/L ZT plus 0.1 mg/L NAA for 15 d, and the induction rate was 100%, the mean No. of buds was 35.7; The combination of MS+0.2mg/L 6-BA +0.1mg/L NAA + 10mg/L AD was the suitable culture medium for elongation of the buds.

以欧洲七叶树成熟种子的胚芽进行离体培养和快速繁殖，结果表明：高约2cm的无菌苗在MS+0.6mg／L 6-BA+0.1 mg／L NAA或MS+0.4～0.6mg／L ZT+0.1 mg／L NAA培养基上培养15 d左右可诱导出不定芽，分化频率为100％，平均每株产芽35.7个；MS+0.2mg／L 6-BA+0.1mg／L NAA+10mg／L AD培养基有利于芽伸长；生根培养基为1／2MS+0.4 mg／L NAA+0.2mg／L IBA时，生根率可达75％。

This paper presents a study of Eucalyptus dunnii tissue culture, using its seeds as explants. The results reveal that MS medium is a suitable basic medium for its seeds to germinate and grow; that MS+KT1.0 mg/L+2, 4-D2.0 mg/L+Homocysteine30mg/L is a good medium to induce its seeds to dedifferentiate directly into callus; that H+6-BA2.0 mg/L+IAA0.2 mg/L is a good medium to make its bud on the seedling reproduce more buds; that MS+6-BA2.0 mg/L+NAA0.2 mg/L is a better medium to induce its root on the seedling into callus; that B5+6-BA2.0 mg/L+2, 4-D0.2 mg/L can induce its under-hypocotyl to differentiate into buds; and that B5+Ad2.0 mg/L+IAA0.2 mg/L can induce its under-hypocotyl into callus to generate normal buds.

以邓恩桉种子为外植体，探讨用最少种子快速繁殖最多幼苗的方法，结果表明：MS培养基是较适合邓恩桉种子萌芽和生长的基本培养基；诱导种子直接脱分化成愈伤组织的较佳培养基配方为MS+KT1.0 mg/L+2，4-D2.0 mg/L+半胱氨酸30 mg/L；以邓恩桉实生苗的芽来繁芽的较理想培养基配方为H+BA2.0 mg/L+IAA0.2 mg/L；邓恩桉实生苗的根诱导愈伤组织的较佳培养基配方为MS+6-BA2.0 mg/L+NAA0.2 mg/L；诱导邓恩桉下胚轴分化芽较佳培养基配方为B5+6-BA2.0 mg/L+2，4-D0.2 mg/L；诱导邓恩桉下胚轴脱分化为胚性愈伤组织的较佳培养基配方为B5+Ad2.0 mg/L+IAA0.2 mg/L。

L~(-1) each, 3.5μL of downstream primer 10μmol ? L~(-1,4μL of RNA,5μL of double-distilled DEPC H_2O;PCR mixture consisted of 20μL of RT-Mixture,5μL of 10 × PCR buffers, 1μL of Taq5U·μL~(-1, 5uL of mixture of dNTPs the 2.5 mmol ?

包括AMV5u·μL~(-11μL，5×Reverse transcriptase buffer 4μL，RNase Inhibitor40U·μL~(-10.5μL，dNTPs混合物10mmol·L~(-1each2μL，下游引物10μmol·L~(-13.5μL，模板4μL，水5μL；PCR反应体系：总反应体系为50μL。

The research on the tissue culture and cell suspension culture showed that thesuitable culture medium for inducing bud was MS supplemented with 1.5mg/L 6-BAand 0.1mg/L NAA, and for the generation-continuing multiplication was MSsupplemented with 1.5mg/L 6-BA and 0.2mg/L NAA. The rooting medium was1/2MS supplemented with 0.5mg/L IBA and the rooting rate was 45.0%. Plantletsurvival after transfer to sand was 52.5%.The induction rate of calli was66.7%～86.6% and the optimum medium was MS medium with 0.5mg/L 6-BA and2.0mg/L 2,4-D. The calli became smallest partical size, friable and had gooddispersion ability after 3 times successive transfer culture, the optimum medium wasMS medium with 0.2mg/L 6-BA and 2.0mg/L 2,4-D. Culturing these particles on sixkinds of MS liquid media with different hormone contents, the optimum medium wasselected basing on he change of the density of single-cell, the density of cellaggrefate and the mass of cell.

对蒜头果进行的组织培养与细胞悬浮培养研究结果表明：MS+6-BA1.5mg／L+NAA0.1mg／L+蔗糖3％激素组合能够较好地诱导芽的初始分化和增殖，适宜的芽苗继代增殖培养基为MS+6-BA1.5mg／L+NAA0.2mg／L+蔗糖3％；采用1／2MS+IBA0.5mg／L+蔗糖3％为生根培养基，生根率为45.0％；移栽到河沙的生根苗成活率为52.5％；愈伤组织诱导率为66.7％～86.6％，其中以MS+6-BA0.5mg／L+2，4-D2.0mg／L+蔗糖3％的培养基最佳，其诱导出的愈伤组织具有较强的增殖能力和较好的脆散结构，最佳继代培养基为MS+6-BA0.2mg／L+2，4-D2.0mg／L+蔗糖3％，且培养基中的6-BA与2，4-D浓度的比值越小，愈伤组织生长越快，结构越脆散，增殖率越高；将继代后的愈伤组织转入6种含不同激素浓度组合的MS液体培养基中进行振荡培养，在综合分析各培养基的单细胞密度，细胞团块密度，细胞生物量增长率等指标后，初步筛选出MS+6-BA0.2mg／L+2，4-D2.0mg／L培养基为较好的液体培养基。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。