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Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of MCSF (25μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed. These cells were also cultured in the presence of both RANKL (30 μg/L) and M-CSF (25 μg/L) with or without 10 μg/L TNF-α for4, 5, 6 and 9 days. The number of TRAP multinucleated cells and resorption pits on dentine slices were counted under light microscope.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞，在含有25μg/L M-CSF和0，1，10，100μg/L TNF-α的α-MEM培养液中培养5d后，观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成；细胞在含有25μg/L M-CSF和30μg/L sRANKL的α-MEM培养液中进行培养，比较加入和不加入10μg/L TNF-α培养4、5、6和9d后，所形成的TRAP多核细胞的数目和骨吸收面积。

The third-generation GS inte l l igent l uxury sports sedan is the first vehic l e to embody the Lexus a l l -new L-finesse design phi l osophy and has deve l oped a different, unique and exciting design direction for Lexus mark.

全新GS 运动型豪华轿车是首款引入LEXUS雷克萨斯全新L－finesse设计理念的批量车型，它完美阐释了LEXUS雷克萨斯个性卓绝而富有激情的未来设计方向。

The results showed that Lufenuron possessed high ovicidal activity on H.armigera 1-day eggs at 50 mg/L,eggs mortality rate was 87.30%;LC50 of stomach poisoning of 2nd ～ 5st instar larvae were 0.7434 mg/L,1.9669 mg/L,2.0592 mg/L and 2.6945 mg/L with leaf dipping method,respectively.

结果表明，虱螨脲具有较强的杀卵作用，在50 mg/L浓度下，棉铃虫1日龄卵死亡率达到87.30%；对棉铃虫2~5龄幼虫具有较高的胃毒活性，其LC50分别为0.743 4 mg/L、1.966 9 mg/L、2.059 2 mg/L和2.694 5 mg/L。

Tamarense was studied. 5 mg·L~-1 of N-phenyl-2-naphthylamine was showed to have 50% inhibitory rate on A. tamarense after 3 days. 70 μL·L~-1 of linoleic acid appeared 40% inhibition rate on A. tamarense.70 μL·L~-1 of pelargonic acid had 85% inhibition rate at the 3 rd day with some increase in cell density later on.

结果表明，浓度高于5 mg·L-1以上的N-苯基-2-萘胺，3 d后能保持对塔玛亚历山大藻抑制率超过50%；浓度为70μL·L-1的亚油酸对塔玛亚历山大藻的抑制率约为40%；浓度为70 μL·L-1的壬酸第3天可以达到85%的抑藻率，但随后藻密度有反弹。

METHODS: Bone marrow was sterilely separated from human. After heparinization, human BMSCs were harvested using density gradient centrifugation and adherence method. At the fifth passage, BMSCs at 1×108/L were incubated in the 6-well plate and divided into 2 groups. BMSCs in the edaravone group were 50% confluent and incubated in L-DMEM containing basic fibroblast growth factor and fetal bovine serum for 24 hours. After washing in PBS, these BMSCs were incubated in serum-free L-DMEM containing 20 mg/L edaravone for 24 hours. BMSCs in the blank control group were incubated in L-DMEM, supplemented with 10% fetal bovine serum.

无菌抽取的骨髓经肝素化后，采用密度梯度离心法及贴壁筛选法分离获得人骨髓间充质干细胞，传至第5代按1× 108 L-1接种于6孔板内，设立2组，依达拉奉组细胞达50%融合时用含碱性成纤维生长因子、胎牛血清的L-DMEM预诱导24 h，PBS洗涤后再用20 mg/L依达拉奉无血清L-DMEM诱导24 h；空白对照组始终用含体积分数为10%胎牛血清的L-DMEM培养，不加任何预诱导剂和诱导剂。

In this study,the muscarinic current was induced by carbachol(50 umol/ L) or GTPYB(0.5 mmol/L).The results showed that hyposmotic superfusate(202mOsmol/ L) increased carbachol-induced current by 145士27%and increased GTPYS-induced current by 183士30%;but in the presence of cytochalasirr B(cyt- B,20 umol/ L),an actin cytoskeleton disrupter,posmotic membrane stretch increased 1CC6 by 70士6%.However,hypos motic membrane tretch induced increase in 1CC6 was potentiated to 545士81% by phalloidin(20 umol/ L),an actin microfilament stabilizer.

当豚鼠胃窦平滑肌细胞的膜电位钳制在一20 m V时，灌流液中50umol/ L卡巴胆碱(carbachol ， CCh)或电极内液中0.5 mmol/L GTPYS均可引导毒覃碱电流，2 m Os mol/ L分别使其增加145士27%和183士30；当电极内液中加入20 umol/ L的细胞松弛B时，低渗牵张使1 cce只增加70士6；而电极内液中加入20 umol/ L的鬼笔环肤则使Icce增加了545士81。

It is found that the best refolding yield for 0.1g/L linear HNTX-Ⅲ could be obtained in double-distilled aqueous solution at pH 7.5 containing 1.0 mol/L L-arginine, 1.0mmol/L reduced glutathione, and 0.1mmol/L oxidized glutathione.

最佳的氧化复性条件为pH 7.5的重蒸水溶液体系，样品质量浓度为0.1mmol/L，还原型谷胱甘肽和氧化型谷胱甘肽的浓度分别为1.0mmol/L和0.1mmol/L、L-Arg浓度为1.0mol/L。

METHODS: The bile was treated with 3 different methods, including the 2D-Clean-up kit group: the bile sample was dissolved with lysis buffer (contains: 7 mol/L carbamide, 2 mol/L thiourea, ammonium persulfate with mass concentration of 0.4 g/L, 65 mmol/L dithiothreitol, carrier ampholytes with mass concentration of 0.2 g/L).

将标本分别按3种不同方法进行处理：①2D-Clean-up试剂盒处理胆汁标本：用裂解液，7 mol/L尿素，2 mol/L硫脲，质量浓度为0.4 g/L的过硫酸铵，65 mmol/L二硫苏糖醇，质量浓度为0.2 g/L的载体两性电解质溶解沉淀过夜。

ES cells were maintained on gelatin-coated dishes in Iscove's modified Dulbecco medium containing 0.1 mmol/L of nonessential amino acids, 2 mmol/L of L-glutamine, 0.1 mmol/L of monothioglycerol, 15% fetal bovine serum, and 1000 U/mL leukemia inhibitory factor. Embryonic bodies were firstly formed by ES cells with hang drip method in IMDM without LIF. Six-day-old EBs were trypsinized and the cells were resuspended at a concentration of （3-4）×109 L-1 cells per 1 mL of a 1:1 mixture of culture medium and Matrigel?

实验方法：将鼠胚胎干细胞株接种于被覆明胶的培养瓶内，加入含0.1 mmol/L非必需氨基酸、2 mmol/L L-谷酰胺、0.1 mmol/L二羟基丙硫醇、15%胎牛血清、1 000 U/mL白血病抑制因子的IMDM培养液。3 d后采用&悬滴法&使细胞在不含白血病抑制因子的上述IMDM培养液中形成胚胎体，6 d后胰酶消化得到胚胎干细胞，浓度调整为（3~4）×109 L-1，与Matrigel？

Separated cultures were isolated from 4 different koumiss examples, the complete 16SrRNA gene of these strains were sequenced, and phylogenetic trees were constructed. The isolateswere identified to the most related strain which had the highest affinity. The identified strains weresix L. lactis, five L. plantarum, five E. faecium, four E. faecalis, two L. casei, two L. helveticus, twoS. thermophilus, one L. delbrueckii subsp, bulgaricus, one Leu. mesenteroides, one Leu. garlicum.

本研究中从4份酸马奶中分离得到了29株乳酸菌，测定了这些乳酸菌的16S rRNA基因全长序列，并构建了乳酸菌属的系统发育树，根据亲缘关系对乳酸菌进行了鉴定，其中L.lactis(6株)，L.plantarum(5株)，E.faecium(5株)，E.faecalis(4株)，L.casei(2株)，L.helveticus(2株)，S.thermophilus(2株)，L.delbrueckii subsp.bulgaricus(1株)，Leu.mesenteroides(1株)，Leu.garlicum(1株)。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。