查询词典 knockout

The choosing of the material and staining of Golgi body and making section;2. To determine the subcellular localization of the encoded protein of a transcriptionally up-regulated gene pdd87in the pten knockout mice,fusion protein expression vector pEGFP-C 1 -pdd87was constructed and transfected into NIH3T3cells and cells were stained with BODIPY TR C 5 -Ceramide to display Golgi body .

为了明确pten敲除小鼠中转录上调基因pdd87编码蛋白在细胞中的定位，构建PDD87与绿色荧光蛋白的融合蛋白表达载体pEGFP-C1-pdd87，利用Lipofectamine2000转染该载体于体外培养的NIH3T3细胞中，采用高尔基体特异探针BODIPYTRC5-Ceramide染色显示高尔基体，激光共聚焦显微镜下观察到PDD87-GFP融合蛋白定位于高尔基体，提示pdd87基因编码的蛋白定位于高尔基体。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。