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The recombinant pET-Lip vector was transformed into E.coliBL21 and induced to express by 1mmol/L IPTG at 37℃. An expected 80kDa fusion proteinwas expressed. SDS-PAGE analysis showed the fusion protein located in the supernatant ofbacteria lysate by sonication. The fusion protein was purified by HisTrap~ HP Kit and coulddegrade tributyrin.

该菌株经IPTG诱导可表达分子量约80 kDa的融合蛋白,SDS-PAGE分析表明融合蛋白位于菌体超声裂解后的上清中,用蛋白纯化试剂盒HisTrap~ HP纯化后得到单一的目的蛋白,约80 kDa,且纯化的融合蛋白具有脂酶活性,可以分解三丁酸甘油酯。

I say excited not in the tumescent, opening-dark-closets way, but in the birthday-present, new-kit sense.

我高兴不是膨胀、打开暗橱的那种感觉,而是打开礼物时的那种新鲜感。

Tunner: You've been to North Africa before, Port. Kit and I will just follow your plan.

特纳:波特,你以前来过北非,凯特和我会紧随你的计划。

Kit Moresby: Yes, Tunner.

凯特·莫斯比:是这样的,特纳。

Objective To evaluate the reliability of ITARON turbidimetric immunoassay reagent kit in measuring C-reaction protein.

目的评价日本CRP试剂盒检测C反应蛋白的可靠性。

If the buyer is not prepared to accept this liability, then he/she should return this kit in new, unassembled, and unusedcondition to the place of purchase.

建议您於初次使用本产品前,先行请教具有相当程度经验同好或是专业雷虎经销商与模型专卖店。

Three age-matched pigs were kept as uninoculated controls.

在攻毒前后分别于耳缘静脉采血,用HerdChek IDEXX Kit测其血清抗体。

We want to hear the sound of your foliage, the unraveling of your tool kit, your songs of loneliness, your songs of hurt.

我们要听来自你叶子的声音,你的工具包的打开,你孤独的歌,你受伤的歌。

A wave of new killer mobile Web applications appear, spurred by the iPhone Software Development Kit and ever more untethered workers.

一波新杀手级移动 web 应用的浪潮出现,由 iPhone 软件开发包 SDK 和更多人员所推动。

The proliferation effect of eight extracts of four Urtica plants on clone MC3T3-E1 cells was examined with cell counting kit-8 (CCK-8) and alkaline phosphatase activity was also determined.

结果三角叶荨麻根的醇提物,宽叶荨麻全草的醇提物和醇提物水层过D101大孔树脂40%洗脱部分在50到200 μg/ml 的浓度下能显著促进成骨样细胞MC3T3-E1增殖。

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推荐网络例句

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?