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All the isolates were first identified by different culture media including Niger seed agar. CACA agar, urease tests, ID32 identification kit, 37℃ growth test, and capability of forming capsule, and then the sequence of the LSUrDNA D1/D2 region were further analyzed.

分别以油菊籽、咖啡酸玉米琼脂、刀豆氨酸-甜菜碱-溴麝香草酚蓝培养基、尿素酶试验、37℃生长试验、ID32生化鉴定板和英膜形成试验等初步鉴定后,再经大亚基核糖体DNA的D1/D2区域序列分析进一步鉴定菌种。

Methods A developed Cetyl Trimethyl Ammonium Bromide method and Qiagen DNeasy plant mini kit were used to extract DNA from papaya seed and fruit.

目的 建立对抗环斑病毒转基因番木瓜55-1进行品系鉴定的检测方法。

Methods: CD34(superscript +) cells from fetal liver were isolated with a magnetic cell sorting kit and were cultured. Cells pretreated with or without protein kinase C inhibitor chelerythrine chloride (3 μmol/L) were induced by 5 ×10^(-7)mol/L tretinoin for 24 h, and then incubated in serum-free medium. Expressions of genes in treated cells were assayed by Western blotting and RT-PCR.

采用免疫磁珠法分离人胎肝CD34细胞,培养4d后,蛋白激酶C特异抑制剂chelerythrine chloride(3μmol/L)处理24h,再加入维A酸处理24h5×10^(-7mol/L,无血清培养基培养5d,Western印迹和半定量RT-PCR法分析维A酸处理前后神经特异基因表达。

Besides, while the auto stop near by the traffic light, the poor person, kit come to request your cherty. You may not feel comfortable at this scene.

此外,小机动三轮车停在近交通号标志边,贫穷的人前来请求您舍钱,您可能对此种环境不适应。

Methods Chlamydozoan antigen was qualitatively determined using colloidal gold technique. Mycoplasma culture and drug susceptibility were carried out with mycoplasma drug sensitivity kit, and the sensitivity of 10 kinds of antibiotics to mycoplasma was analyzed.

应用衣原体试剂盒定性检测衣原体抗原,支原体培养及药敏选用支原体药敏试剂盒,并对10种抗生素对支原体的敏感性进行了分析。

Methods SMMC-7721 cell apoptosis was induced by HCPT and the mitochondria were isolated with a mitochondria isolation kit. Mitochondrial proteins labeled with a cleavable isotope-coded affinity tag were identified and quantified using two-dimensional liquid chromatography/tandem mass spectrometry.

用羟基喜树碱诱导肝癌细胞SMMC-7721凋亡,提取线粒体并鉴定其纯度;用可裂解的同位素标记的亲和标签技术标记线粒体蛋白质,利用二维液相色谱-串联质谱分析鉴定蛋白质。

For instance: visit www.4 cleanP.com and be amazed. For $69 you can buy a concealable urine substitution kit with enough human urine for two urinalysis tests.

访问 www.4 cleanP.com 和吃惊的是,你用$69就能买可隐蔽尿替代物包,提供两次尿分析试验足够的人尿。

One of the things I hated about making this kit the first time was the flimsy railings and crapy masts.

其中的件我恨约,使这个工具包的第一时间,是站不住脚的栏杆和crapy桅。

METHODS: The BEC were preincubated with crocetin (1, 0.1 and 0.01 μmol/L) for 12 h, then exposed to AGE (100 mg/L). The RAGE mRNA expression was detected by RT-PCR analysis, the intercellular adhesion molecule-1(ICAM-1) was measured by ELISA. The extracellular superoxide ion and thiobarbituric acid reactive substances were assessed with superoxide ion kit and colorimetric assay, respectively The intracellular I2O2 was also detected using the probe 2,7-dichlorofluorescein. The mitochondrial membrane potential and mitochondrial Succinate dehydrogenase were analyzed by the retention of rhodamine 123 (Rh123) and MTT.

不同浓度的西红花酸(1、0.1、0.01μmol/L)预孵BEC细胞12h后,用AGE(100mg/L)刺激细胞12h,RT-PCR法测定RAGEmRNA的表达水平;ELISA法测定细胞间黏附分子-(ICAM-1)的表达;试剂盒分别检测胞外超氧阴离子和硫代巴比妥酸反应产物浓度;同时,还用2,7-二氯荧光素测定了胞内H2O2的浓度,并用罗丹明123(Rh123)荧光法及MTT法分别检测细胞线粒体膜电位水平和其琥珀酸脱氢酶的活性。

Total mRNA was extracted from a venom gland of snake Daboia russellii siamensis following the manufacturers protocol of the PolyATtract System 1000 kit purchased from Promega Biotech. cDNAs encoding C-type lectins were amplified by RT-PCR and subcloned into a pMD18-T vector.

按照Promega公司的mRNA提取试剂盒操作手册,从圆斑蝰蛇的毒腺中提取mRNA;利用RT-PCR的方法进行体外扩增,获得C-型凝集素蛋白的基因,克隆到pMD18-T载体中。

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推荐网络例句

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势,并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。

This dramatic range, steeper than the Himalayas, is the upturned rim of the eastern edge of Tibet, a plateau that has risen to 5 km in response to the slow but un stoppable collision of India with Asia that began about 55 million years ago and which continues unabated today.

这一引人注目的地域范围,比喜马拉雅山更加陡峭,是处于西藏东部边缘的朝上翻的边框地带。响应启始于约5500万年前的、缓慢的但却不可阻挡的印度与亚洲地壳板块碰撞,高原已上升至五千米,这种碰撞持续至今,毫无衰退。