查询词典 kinases

The expressed protein concentrates were about 0.5-5mg/L. 3. Classification and functional annotation of kinases have been studied: according to the kinases Classification in PlantsP (http://plantsp.genomics.purdue.edu/), 27 kinases belong to 7 groups in the class 1 of Transmembrane Receptor Kinase and Related non-Transmembrane Kinases on PlantsP, namely Receptor like cytoplasmic kinaseⅦof group 1.2, CRPK1 Like Kinase of group 1.3, Crinkly 4_Like kinase of group 1.4, Plant Extemal Response Like Kinase of group 1.6, CRPK1 Like Kinase (Types 1 and ) and S-Domain Kinase (Type 2) of group 1.9, Legume Lectin Domain Kinase of group 1.11, Wall associate kinase of group 1.5, respectively. 4. 21 kinases expressed in S.

对获得的蛋白激酶进行了分类和功能预测：按照PlantsP网站(http：//plantsp.genomics.purdue.edu/)的激酶分类系统，本实验表达的激酶分别属于第一类跨膜受体激酶和无跨膜区的激酶中的7个不同组，即第2组的Receptor LikeCytoplasmic KinaseⅦ家族、第3组的CRPK1L(Type 1)家族、第4组的Crinkly 4_Likekinase家族、第6组的Plant External Response Like Kinase家族、第9组的CRPK1 LikeKinase(Types 1 and 2)和S-Domain Kinase(Type 2)家族、第11组的Legume LectinDomain Kinase家族和第17组的Wall Associated Kinase kinase家族； 4。

Here we show that phenylarsine oxide and diamide (both were inhibitors for protein tyrosine phosphatases), but not genistein (an inhibitor for protein tyrosine kinases), adenosine, wortmannin and LY294002 (all were inhibitors for phosphatidylinositol 3- and 4-kinases), could inhibit P-selectin exocytosis on activated platelets and could abolish the P-selectin mediated aggregation of activated platelets to neutrophils.

我们发现蛋白酪氨酸磷酸酶的抑制剂phenylarsine oxide和diamide能够抑制P-选凝素从活化的血小板中外排出来，并能够抑制P-选凝素介导的活化的血小板在白细胞表面的粘附，而蛋白酪氨酸激酶的抑制剂genistein，磷脂酰肌醇3-或4-激酶的抑制剂adenosine、wortmannin和LY294002在这些实验中没有作用。

Here we show that phenylarsine oxide and diamide (both were inhibitors for protein tyrosine phosphatases), but not genistein (an inhibitor for protein tyrosine kinases), adenosine, wortmannin and LY294002 (all were inhibitors for phosphatidylinositol 3-and 4-kinases), could inhibit P-selectin exocytosis on activated platelets and could abolish the Pselectin mediated aggregation of activated platelets to neutrophils.

我们发现蛋白酪氨酸磷酸酶的抑制剂phenylarsine oxide和diamide能够抑制P-选凝素从活化的血小板中外排出来，并能够抑制P-选凝素介导的活化的血小板在白细胞表面的粘附，而蛋白酪氨酸激酶的抑制剂genistein，磷脂酰肌醇3-或4-激酶的抑制剂adenosine、wortmannin和LY294002在这些实验中没有作用。

Because the signaling molecules RAS and ERK1/2 (extracellular signal–regulated kinases 1 and 2) are activated by an LH surge in granulosa cells of preovulatory follicles, we disrupted Erk1/2 in mouse granulosa cells and provide in vivo evidence that these kinases are necessary for LH-induced oocyte resumption of meiosis, ovulation, and luteinization.

研究人员仍然在试图解析接着发生的那些导致排卵（或在某些情况下没有排卵）的分子信号级联反应。研究人员现在证明，激酶ERK1和ERK2是该激素信号的至关重要的下游标靶。在那些颗粒细胞中缺乏这些酶的雌性小鼠中，其卵母细胞不会成熟，因而不会从它们的滤泡中释放出来。

For most viruses,there is aneed for antimicrobials that target unique viral molecular properties.Acycloviris one such drug.It is activated into ahuman herpesvirusDNA polymerase inhibitor exclusively by HHV kinases and,thus,does not suppress other viruses.Here,we show that ACV suppresses HIV-1in HHV-coinfected human tissues,but not in HHV-free tissue or cell cultures.However,addition of HHV-6-infected cells renders these cultures sensitive to anti-HIV ACV activity.We hypothesized that such HIV suppression requires ACV phosphorylation by HHV kinases.Indeed,an ACV monophosphorylated prodrug bypasses the HHV requirement for HIV suppression.Furthermore,phosphorylated ACV directly inhibits HIV-1reverse transcriptase,terminating DNA chain elongation,and can trap RT at the termination site.These data suggest that ACV anti-HIV-1activity may contribute to the response of HIV/HHV-coinfected patients to ACV treatment and could guide strategies for the development of new HIV-1RT inhibitors.

对大多数病毒而言，都需要有针对其分子特性的靶向杀毒剂阿昔洛韦就是这样一种靶向药物在人疱疹病毒酶的特定作用下，阿昔洛韦被激活成为人疱疹病毒DNA聚合酶抑制剂，因此不能再抑制其它的病毒我们的研究发现阿昔洛韦在共感染人疱疹病毒的组织中可以抑制HIV-1，但在无人疱疹病毒感染的组织或细胞中无此作用然而，加入人疱疹病毒-6感染的细胞却使得其对抗HIV的阿昔洛韦变得敏感我们推测这种抑制作用依赖于人疱疹病毒酶导致的阿昔洛韦磷酸化实际上，单磷酸化的阿昔洛韦前体药物无需人疱疹病毒的参与即可抑制HIV此外，磷酸化的阿昔洛韦能直接抑制HIV-1逆转录酶，将其阻止在终止位点，从而终止DNA链的延长这些结果提示阿昔洛韦的抗HIV-1活性决定了艾滋病病毒/人疱疹病毒共感染的患者对阿昔洛韦的治疗反应，也有助于开发新的HIV-1逆转录酶抑制剂

The largest group of kinases are Protein kinases, which act on and modify the activity of specific proteins.

蛋白激酶是数量最多的一类激酶，作用和修饰特定蛋白的活性。

Based on rice protein kinase sequences obtained from the database, kinases of functional representative and intron-free within gene were selected, 32 pairs primers were designed in this experiment; coding sequences of these kinases were obtained by PCR and cloned into yeast expression vector pEGH. GST-fusion proteins were expressed in Saccharomyces cerevisiae Y258, and their autophosphorylation activities were detected furthermore.

本实验利用公布的水稻激酶序列，选取功能有代表性并且其基因内部没有内元的32个序列，通过PCR扩增获得水稻激酶区的编码序列，克隆于酵母表达载体pEGH上，在酿酒酵母Y258菌株中表达融合蛋白质，并进行了自我磷酸化活性分析。

Degenerate primers based on the sequences of conserved subdomains VIB and IX from Cdc2-related kinases were used for screening novel Cdc2-related kinases.

根据Cdc2蛋白激酶保守亚结构域VIB和XI的氨基酸序列设计合成简并引物，以喉癌mRNA为模板合成cDNA，然后在非严紧退火条件下进行简并PCR。

We identified 53 genes encoding protein Ser/Thr or Tyr kinases on the genome of Anabaena sp.

PCC 7120中Ser／Thr或Tyr蛋白质激酶功能成为可能。我们在Anabaena sp。

Results of some laboratory studies of genistein have also been mixed, but most have shown favorable results, Bergan said, demonstrating that genistein can inhibit a variety of cell molecules including tyrosine kinases, which activate proteins by attaching them to phosphate chemicals.

结果，一些实验室的研究金雀异黄素也有好坏参半，但大多数表现出良好的结果，伯根说，这表明金雀异黄素能抑制多种细胞的分子，包括酪氨酸激酶，激活蛋白质将它们附加磷酸盐的化学品。

Dixie itself is a nickname for the American South.

Dixie 本身是美国南部的别称。

I think John Terry epitomises that and a big reaction is what we will be looking for.

而我认为特里就是这样一个典型，他正是我们期望看到的那个领秀。