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Catenin, ER-β or Ki-67 can not predict the prognosis of desmoid-type fibromatosis.

catenin、ER-β和Ki-67均不能作为韧带样型纤维瘤病的预后指标。

Methods Nuclear β-catenin expression was detected by immunohistochemistry in 77 lesions with desmoid-type fibromatosis and 171 other spindle cell lesions, including superficial fibrmoatosis (n=18), nodular fasciitis (n=36), keloid (n=16), scar (n=10), granulation tissue (n=9), synovial sarcoma (n=38), neufibroma (n=13), solitary fibrous tumor (n=12), gastrointestinal stromal tumor (n=10), low-grade myxofibrosarcoma (n=3), low-grade fibromyxoid sarcoma (n=3), and smooth muscle tumor (n=10). In addition, the immunohistochemical expressions of ER-α, ER-β and Ki-67 were examined in all of the lesions with desmoid-type fibromatosis. The nuclear immunohistochemical staining for nuclear βcatenin and ER-β was graded as high level (≥25% of cells), low level (5%-25%) or none.

采用免疫组织化学染色法检测77例韧带样型纤维瘤病和171例其它良、恶性梭形细胞病变(包括结节性筋膜炎36例、浅部纤维瘤病11例、瘢痕疙瘩16例、增生性瘢痕10例、肉芽组织9例、滑膜肉瘤38例、神经纤维瘤13例、孤立性纤维性肿瘤12例、胃肠间质肿瘤10例、低度恶性黏液纤维肉瘤3例、低度恶性纤维黏液样肉瘤3例及平滑肌肿瘤10例)组织中β-catenin核阳性的表达,同时检测韧带样型纤维瘤病中ER-α、ER-β和Ki-67的表达。

Methods Human hemangioma endothelial cells cultured in vitro were injected hypodermically into nude mice(BALB/c nude mice). The volume of in the transplanted tumor was measured in 60 days. Samples were taken from survival tumor and stained immunohistochemically by CD31 and Ki-67 before subjected to histopathological examination.

将体外培养的血管瘤内皮细胞通过注射的方式植入裸鼠(BALB/c nude mice)皮下,测量不同生长时期瘤体体积变化,观察至60天后进行病理学光镜检查,并通过血管内皮细胞单克隆抗体CD31、细胞增殖标记抗体Ki-67免疫组化染色研究所成瘤体的同源性及增殖活性。

The results of absorption spectra, KI fluorescence quenching studies, competitive binding studies and thermal denaturation experiments suggested that the interaction between AQS and DNA be intercalative.

在CT DNA存在下,KI对AQS光化学反应产物的荧光猝灭常数K〓比没有CT DNA存在情况下的要小。2.5×10〓mol/L的溴化乙锭即可将3.2×10〓mol/L的AQS从AQS与DNA的结合物中完全置换出来。

Deletion of p16 gene and subsequent irregulation of cell cycle play an important role in the occurrence and development of gastric carcinoma.Detection of p16 protein will contribute to the prognosis and therapy of gastric neoplasm.Both p16 and Ki-67 may help to predict the possibility of lymth node metastasis in gastric carcinomas.

p16蛋白表达缺失及其引起的细胞周期调控失常在胃癌的发生、发展中起一定作用;p16蛋白的检测有助于估计胃癌患者的预后、指导临床治疗;联合检测p16和Ki-67对判断淋巴结转移的危险性具有较高的价值。

3The transplantation tumor karyon morphology, number of apoptotic cells, micro vascular density and the gene expression of Ki-67, iNOS, MMP-2 in each group were quantitatively detected and compared by the application of pathomorphology, immunohistochemistry and image analysis technique.

3采用病理形态学、免疫组化、图象分析技术定量检测并比较各组移植瘤细胞核形态、凋亡细胞数、微血管密度以及Ki-67、iNOS、MMP-2的基因表达情况。

Objective:To study the expression and regulation of proliferating cell nuclear antigen、Ki-67、vascular endothelial growth factor、c-myc、bax and bcl-2 in three lymphadenectasis diseases,including lymphadenitis、reactive hyperplasia of lymph node、malignant lymphoma.

目的:探讨增殖细胞核抗原、Ki-67、血管内皮生长因子、c-myc、bax及bcl-2在淋巴结炎症性肿大、增生性肿大、肿瘤性肿大三大类淋巴结肿大疾病中的表达水平和规律。

There were 8 patients in the macromastia group and 5 patients in the control group. The result didn't show significant difference between the two groups. There is no difference between the two groups in mammary epithelial cell size measurement and Akt-1/2/3 immunohistochemistry stain. The levels of Ki-67, phospho-Akt-1/2/3, and STAT5a of juvenile macromastic patients are higher than those of the patients of the control group, and the results are statistically significant.

三年内共搜集到八位巨乳症病患及五位对照组,两组以Wilcoxan rank sum test比较,乳腺上皮细胞的尺寸及Akt-1/2/3免疫组织化学染色方面在统计学上的没有显著的差异,而乳腺上皮细胞之Ki-67、phospho-Akt-1/2/3、及STAT5a免疫组织化学染色方面,则均为青春期巨乳症患者组较高,统计学上均有显著性的差异。

Juvenile macromastia ; mammary epithelial cell ; cell size ; proliferation marker ; immunohistochemistry stain ; Ki-67 ; Akt-1/2/3 ; STAT5a

中文关键字青春期巨乳症;乳腺上皮细胞;细胞尺寸;细胞增生分子标记;免疫组织化学染色; Ki-67 ; Akt-1/2/3 ; STAT5a

Paquet says one major aspect of the movie's power is its cinematography, by newcomer Hwang Ki-seok."This has to be one of the most gorgeous Korean films of recent years, from cityscapes that resemble watercolors to the graceful shattering of windows and doors."

Paquet 称影片的一个主要的成功因素是它的拍摄技术,这主要是新人Hwang Ki-seok 的功劳,&这是近年来韩国电影中其中一部最棒的影片,从水彩画般的城市风光到打破门窗的画面都棒极了。&

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