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The apoptosis of 80 mg/kg ﹒ d group was mainly located in spermatogenous cell and first spermatocyte; the apoptosis of 120 mg/kg ﹒ d group was mainly located in spermatogenous cell , first spermatocyte and spermatocyte of the second order; the apoptosis of 240 mg/kg ﹒ d group was mainly located in spermatogenous cell and first spermatocytes, and seemingly, the apoptosis located in first spermatocyte was dominant; whereas the apoptosis of 480 mg/kg ﹒ d group was located in all levels spermatogenic cells.

结果表明:各试验组小鼠生精细胞凋亡指数随着浓度的升高有增加的趋势,而且与对照组差异显著( P <0.05或 P <0.01)。80 mg/kg · d 组凋亡细胞主要定位于精原细胞和初级精母细胞;120 mg/kg · d 组凋亡细胞主要定位于精原细胞、初级精母细胞和次级精母细胞;240 mg/kg · d 组凋亡细胞主要定位于精原细胞、初级精母细胞,以初级精母细胞为最多;而480 mg/kg · d 组凋亡细胞定位于各级生精细胞。

To explore effect of copper on MPTP in liver cell, performance, blood index, hepatic function, growth hormone and histopathological change, copper sulfate, copper methionine and tribasic copper chloride had been used in this experiment, level of copper was 11mg/kg in control group, the three levels of each copper sulfate, copper methionene and TBCC added to the basas diet were 110mg/kg, 220mg/kg and 330mg/kg copper in experimental groups.

为了全面了解肉鸡饲料中铜元素对其肝细胞线粒体膜通透性转变孔、生产性能、血常规、肝功能、生长激素以及组织病理学变化的影响,本试验选用:硫酸铜、蛋氨酸铜、三碱基氯化铜作为铜源,基础日粮对照组饲料铜含量为Feedstuff推荐的11mg/kg,三个试验高铜组分别为:110mg/kg、220mg/kg、330mg/kg.360羽1日龄AA商品代肉鸡随机分为12组,每组30羽。

Male adult rats were randomly divided into five groups,which were treated via gastric gavage of distilled water(1 mL·kg-1),solution of GTW(10 mg·kg-1) and three doses of water decoction of root of C.cuneata (1.8,5.4,18 g·kg-1)+ GTW(10 mg·kg-1),respectively.8 weeks later,GTW was stopped and the decoction and water continued for another 4 weeks.And then,all the male rats were copulated with adult female rats.

将成年雄性大鼠随机分组,分别给予雷公藤多苷片溶液(10 mg.kg-1),野山楂根水煎剂(1.8,5.4,18 g.kg-1)+雷公藤多苷片溶液(10 mg.kg-1)和蒸馏水(1 mL.kg-1)灌胃,8周后停用雷公藤多苷,中药组继续行野山楂根灌胃。4周后各组动物与成年雌性大鼠交配,观察交配雌鼠妊娠率和平均胚胎、黄体数。

Results show that microbial biomass carbon and microbial biomass nitrogen in fertilized soil peak in the first several days, and then decline. Intercropping Incubating both sweet maize straw and white clover have the highest yield, maxima of 529.57 mg·kg-1 and 75.50 mg·kg-1 respectively. They also release the most respired CO2 the most among all treated soils. The best mineral nitrogen release is in white clover intercroppingincubating, with a maximum of 29.81 mg·kg-1 under a 26-day culture, and then keeping 1.60 times the control and reaching 2.48 times at 80-day culture. Sweet maize incubatingintercropping has the least mineral nitrogen release. It is 13%~53% of the control under a 26-day culture, with a maximum of 7.51 mg·kg-1. Soil nitrogen mineralizatiomineralization is insignificant under sweet maize incubatingintercropping with urea in short-term.

研究发现,各施肥处理的土壤微生物量碳、微生物量氮均在培养前期出现峰值,后期平稳降低;甜玉米秸秆和白三叶草绿肥同时还田的土壤微生物量碳、微生物量氮在各培养时期均最大,峰值分别达529.57 mg·kg-1和75.50 mg·kg-1,土壤呼吸产生的CO2最多;白三叶草绿肥单独还田有利于土壤无机氮的释放,培养第26 d 无机氮达到最大值,为29.81 mg·kg-1,之后一直在对照处理的1.60倍以上,第80 d达到2.48倍;甜玉米秸秆单独还田不利于土壤无机氮的释放,培养的第26 d至结束,甜玉米秸秆处理的无机氮为对照的13%~53%,最大为7.51 mg·kg-1;甜玉米秸秆配施尿素,短期内不利于土壤无机氮矿化。

RESULTS : Carbamazepine (25–60 mg/kg; p.o.), oxcarbazepine (10–40 mg/kg; p.o.), gabapentin (10–70 mg/kg; p.o.), and topiramate (5–30 mg/kg; p.o.) caused a significant dose-dependent reduction the number of writhes in the writhing test.

结果:口服醯胺咪嗪25–60 mg/kg )、奥凯西平(10–40 mg/kg 、加巴喷丁(10–70 mg/kg )和托吡酯(5–30 mg/kg )后,在扭体试验中会使小鼠翻腾次数以剂量依赖的方式减少。

Methods Mice were randomly divided into 6 groups:normal saline group, OMT groups with different doses (50,100,and 200 mg·kg-1), pethidine hydrochloride (10 mg·kg-1)group, and NG-nitro-L-arginine methyl ester 20 mg·kg-1+OMT 100 mg·kg-1 group.

将小鼠随机分成6组,即生理盐水组,OMT 50、100和 200 mg·kg-1组,盐酸哌替啶注射液(10 mg·kg-1)组,L-硝基-精氨酸甲酯20 mg·kg-1+OMT 100 mg·kg-1组。

Methods: Sixty patients in epidural anesthesia of gynecological surgery were randomly divided into two groups(n=30). Group A midazolam 0.04 mg/kg and fentanyl 1.0 μg/kg; group B: pethidine 1.0 mg/kg and haloperidol 0.05 mg/kg.

选择ASA 1~2级硬膜外麻醉下行妇科手术患者60例,随机分成两组,每组30例。A组:咪唑安定0.04mg/kg+芬太尼1.0μg/kg;B组:哌替啶1.0mg/kg+氟哌利多0.05mg/kg。

Results showed that low contents of Cd has promotion effect on Oryza Sativa, but high contents of Cd has not obvious restrain on it. The total trend of distribution of Cd in Oryza Sativa is root>stem>shell>rice. The relation between Cd contents in rice and total Cd in soil as well as availability Cd in soil has been investigated. The result showed that both the total Cd and the availability Cd had notably correlation with content of Cd to rice, and the critical point were CaCl2-Cd=0.71mg/kg, DTPA-Cd=1.34 mg/kg, EDTA-Cd=0.76 mg/kg, NH4OAc-Cd=0.77 mg/kg.

研究表明:低浓度的镉对水稻生长稍有促进作用,高浓度的镉对其生长并没有明显的抑制作用;镉在水稻植株中总的分布趋势为根>茎>稻壳>糙米;用4种有效态提取剂(0.1 mol/L CaCl2、DTPA、0.05 mol/L EDTA和1 mol/L NH4OAc)浸提出的有效态镉的含量和土壤中镉总量与水稻糙米中镉含量均达到显著相关水平,并确定了它们的临界值分别为CaCl2-Cd=0.71 mg/kg, DTPA-Cd=1.34 mg/kg, EDTA-Cd=0.76 mg/kg, NH4OAc-Cd=0.77 mg/kg。

Methods The drug is given to rhesus monkey by the intramuscular injection, first dose duplicate followed by a single dose at 24,48,72,and 96hrs for 5 days, the total doses are given respectively as 15.0mg/kg, 7.2mg/kg, 3.6mg/kg, 1.8mg/kg. Experimental animal was bitten by Anopheles dirus before giving drug, and at 6,12,24,30,36,48,54,60,72,84,and 108hs later, mosquites were examined for oocysts on day 9 and sporozoites on day 13 after infection.

方法首剂药量加倍,随后24,48,72和96小时给实验猴各肌注一次,疗程5天,总剂量分别为15.0mg/kg、7.2mg/kg、3.6mg/kg、1.8mg/kg;在给药前及给药后6、12、24、30、36、48、54、60、72、84和108小时各用一批大劣按蚊叮咬实验猴,叮咬后第9天解剖蚊胃,第13天解剖涎腺。

A method for simultaneous determination of oxytetracycline, tetracycline, chlortetracycline and choramphenicol in seafood was successfully developed using high performance liquid chromatography with ammonium acetate/water and acetonitrile as the mobile phase. The results showed that the four antibiotics were well separated under the optimum conditions, with the limits of detection being 32 μg/kg for oxytetracycline, 15 μg/kg for tetracycline, 23 μg/kg for chlortetracycline, and 19 μg/kg for choramphenicol. The recovery of standard additions was 76~92% and the relative standard deviation was 0.8-4.5%. The method is rapid, sensitive, accurate and reliable and can be applied in the analysis of antibiotic residues in seafood.

建立了同时检测海产品中土霉素、四环素、金霉素、氯霉素残留的高效液相色谱分析方法,以醋酸铵-醋酸/水缓冲液和乙腈作为流动相,通过二元梯度洗脱,使四种抗生素获得了较好的分离,以基线噪音的3倍计算,土霉素、四环素、金霉素、氯霉素最低检出浓度分别为32μg/kg、15μg/kg、23μg/kg、19μg/kg,在添加浓度范围内,四种抗生素的加标回收率为76~92%,相对标准偏差为0.8~4.5%,该方法快速、灵敏、准确,可靠性高,可用于海产品中抗生素残留的色谱分析。

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