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Propofol group and control group 0.4 mg/kg propofol was administrated intravenous when the patient was awaken from an esthesia,as to the control group,2 mL saline was administrated as placebo.

70例ASAI~II级择期腹腔镜下妇科手术患者,随机分为实验组和对照组,实验组在麻醉苏醒后静脉注入异丙酚0.4 mg/kg,对照组使用生理盐水2 ml。

Rabbit anti-thrombosis in oral fungus polysaccharides 18.5mg/kg, can significantly extend the specificity of thrombus and fibrin thrombus formation time and shorten the length of thrombus, reduce the thrombus wet weight and dry weight, reduce platelet count, lower platelet adhesion rate and blood viscosity, and can significantly shorten the guinea pig euglobulin lysis time, lower plasma fibrinogen levels, elevated plasmin activity, results showed that the fungus polysaccharides significantly antithrombotic effect.

抗血栓形成兔口服木耳多糖18.5mg/kg,可明显延长特异性血栓及纤维蛋白血栓的形成时间,缩短血栓长度,减轻血栓湿重和干重,减少血小板数,降低血小板粘附率和血液粘度,并可明显缩短豚鼠优球蛋白溶解时间,降低血浆纤维蛋白原含量,升高纤溶酶活性,结果表明,木耳多糖有明显的抗血栓作用。

Rabbit anti-thrombosis in oral fungus polysaccharides 18.5mg/kg, can significantly extend the specificity of thrombus and fibrin thrombus formation time and shorten the length of thrombus, reduce the thrombus wet weight and dry weight, reduce platelet count, lower platelet adhesion rateand blood viscosity, and can significantly shorten the guinea pig euglobulin lysis time, lower plasma fibrinogen levels, elevated plasmin activity, results showed that the fungus polysaccharides significantly antithrombotic effect.

抗血栓形成兔口服木耳多糖18.5mg/kg,可明显延长特异性血栓及纤维蛋白血栓的形成时间,缩短血栓长度,减轻血栓湿重和干重,减少血小板数,降低血小板粘附率和血液粘度,并可明显缩短豚鼠优球蛋白溶解时间,降低血浆纤维蛋白原含量,升高纤溶酶活性,结果表明,木耳多糖有明显的抗血栓作用。

Objective:To evaluate effect of 5μg/kg fentanyl on catecholamine and cardiovascular responses to explantation in brain-dead organ donors.

目的:观察5μg/kg芬太尼对脑死亡供体器官摘除期间儿茶酚胺和心血管反应的影响。

METHODS: Liver fibrosis models were established in 24 SD rats by intragastric administration (7 g/kg) of alcohol (56°, 560 mL/L) every morning, and 10 normal rats served as controls. The rats in model group were killed by exsanguination from femoral vein at 4, 12 and 24 wk. Pathological changes of liver tissues in rats were identified by Masson staining, and the expression of VEGF mRNA was detected by semiquantitative reverse transcription-polymerase chain reaction.

56度的白酒(560 mL/L)平均以7 g/kg的剂量每日早晨灌胃一次制备肝纤维化模型,灌胃4 wk、12 wk及24 wk采用股静脉放血法分别处死大鼠,观察肝脏病理变化并采用半定量逆转录聚合酶链反应检测VEGF mRNA的表达。

Lambs were allotted to a 3 x 3 factorial experiment with three levels of vitamin E 0,500 and 1000IU day~(-1 lamb~(-1) and three levels of selenium (0,0.2 and 0.4mg/kg).

采用两因子三水平完全随机区组试验设计,将维生素E和有机硒作为两个因子,设3个维生素E补饲水平(0、500和1000IU)和3个有机硒补饲水平(0、0.2和0.4mg╱kg)。

Methods: Experimental rats were injected with KA (10mg/kg, 5mg/ml) subcutaneously to make status epilepticus model. SE latter 10 day, Use subliminal dosages KA to check falling sickness paroxysm sensibility model; SE latter 10 day , Morris water maze was to evaluate cognitive function in the animals. Changing NPY adopt immunocyte chemistry method to check the rat brain inner is expressed.

本研究给Sprague-Dawley大鼠颈部皮下注射惊厥剂量的红藻氨酸(KA,10mg/kg,5mg/ml),诱发癫痫持续状态(status epilepticus,SE), 10天后,用阈下剂量的KA检测癫痫发作敏感性模型;10天后用Morris水迷宫实验检测动物学习记忆功能变化;用免疫组化技术检测动物海马CA1区CA3区NPY表达的变化。

Rabbits received naloxone HCl intracerebroventricularlyor naloxone methiodide IV, a quaternary compound that does notcross the blood–brain barrier, 5 min before FENT treatment(5 or 50 g/kg, IV).

使用 FENT 治疗(5或50 g/kg ,静脉注射)前5min ,向家兔脑室内注射盐酸纳洛酮或静脉注射甲硫丁氨酸纳洛酮(一种四元化合物,不能透过血脑屏障)。

The flic gene of App was obtained by PCR based on highly homology with Escherichia coli, Salmonella, Shigellosis. Then it was legated to pMD-18T vector. The recombinant plasmid was identified by enzyme and sequence analysis. The 528 bp fragment of flic gene was successfully cloned into pGEX-KG vector, then the recombinant plasmid was transformed into E.

以猪传染性胸膜肺炎放线杆菌血清Ⅰ型菌株基因组为模板,根据其鞭毛区与其它细菌高度同源性设计引物,利用PCR方法扩增鞭毛蛋白基因flic片段,将其亚克隆到pMD-18T载体中并进行PCR和酶切鉴定,再将其亚克隆与载体pGEx-kG分别双酶切和连接,构建重组表达载体pGEX-flic,并将其转化大肠杆菌工程菌BL21,进行原核表达。

Square of the maximum flux density (kg 2)b.

最大磁通密度(kg 2)b。的平方。

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