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The ANP group was given 6 hourly injections of a supramaximal dose of cerulein (50 μg/kg). After the final cerulein injection, LPS was injected intraperitoneally at a dose of 10 mg/kg. The control group was given the same volume of normal saline solution. Three hours after the final injection, mice were sacrificed.

模型组小鼠腹腔内注射雨蛙素(50 μg/kg)，连续6次，每次间隔1 h，在末次注射雨蛙素后，即向小鼠腹腔内注射脂多糖(10 mg/kg)；对照组小鼠腹腔内注射等体积生理盐水。

In the long-period toxicity test, DCG was administered repeatedly for 60 days, the dose were 72.6,36.3,18.2 g/kg respectively(It refered to the dose of material,which amounted to 52.6,26.3,13.2 times of 50 kg human clinic use). There is no other abnormal changes about color patem,action,urine and stool,but cibation, hydroposia, body weight and index part of hematological and hematobiochemical parameters,coefficient and histomorphological figure of organs were examined during administration phase.The rats were examined in 21 days after the cessation of DCG, the abnormal changes of index was completely recovered.

定喘颗粒大鼠给药60 d的毒性试验，剂量分别为72.6，36.3，18.2 g/kg（相当于临床50 kg人用量的52.6，26.3和13.2倍），给药期间各组大鼠毛色、活动、二便均无异常变化，但给药组大鼠摄食、饮水、体重以及部分血液学、血液生化学指标和脏器系数有异常变化，停药后，上述异常变化指标均已恢复正常。

GdCl3(10mg/kg) or the same volume of NS was continually injected of vein at 48h and 24h before LPS(5mg/kg) was injected in the male kunming species mouse. Then took out of liver or isolated KCs 30 minute after LPS was injected and assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of liver or KCs in vivo.Ⅱ. Isolated and cultured KCs of mouse, 1 h pretreatment with GdCl3; Culture medium with LPS(100ng/ml) was added and continuatively incubated 30 minute. Assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of KCs in vitro and detection effect of GdCl3 on its phagocytosis function in respectively.

Ⅰ 雄性昆明种小鼠在注射LPS（5mg/kg）前48h、24h，分别静脉注射GdCl3（10mg/kg ）或等量的生理盐水，于LPS或生理盐水注射后30min，分别取出肝脏或分离KCs，检测肝脏或KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平；Ⅱ体外培养小鼠KCs经GdCl3（100μM）预处理1h，加入含LPS（100ng/ml）的DMEM培养基继续孵育30min，分别检测体外培养KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平和GdCl3对其吞噬、分泌功能的影响。

Methods Myocardial ischemia was induced by subcutaneous injection of ISO (30 mg/kg for two days, once a day). The experimental animals were randomly divided into the normal group, ISO group, positive control group and three crocetin groups (25, 50, 100 mg/kg). Cardiac indexes were examined. The level of LDH, CK, MDA in serum were measured. The content of MDA, GSH-Px and the ATPase activity heart and mitochondria were assayed by colorimetric analysis. The histopathological change of myocardia was investigated by HE staining.

采用大剂量ISOsc诱导产生大鼠急性心肌缺血模型(1SO30mg／kg，每日一次，连续2d)，实验大鼠随机分为正常组、ISO模型组、阳性对照组和3个西红花酸给药组(25，50，100mg／kg)，测定心脏系数和血清中乳酸脱氢酶、磷酸肌酸激酶和丙二醛含量，观察心肌组织谷肮甘肤过氧化物酶、Na^ ，K^ —ATP酶和Ca^2 ，Mg^2 —ATP酶活性及心脏病理改变，观察西红花酸的保护作用。

Methods: Uses carbon tetrachloride (CC14) 2ml/kg, thioacetamide 200mg/kg, the abdominal cavity to inject separately, Establish model which the rat acute liver damages; Guarantees the liver profit stomach mixture, the polyene phosphatide acid radical choline capsule, the glycyrrhizic acid diamidogen capsule fills the stomach.

分别用四氯化碳(CC14)2mL/kg，硫代乙酰胺200mg/kg，腹腔注射，建立大鼠急性肝损伤的模型；保肝益胃合剂，多烯磷脂酰胆碱胶囊，甘草酸二胺胶囊灌胃治疗。

One hundred and fifty 28-day-old crossbred piglets (Duroc×Landrace×Yorkshire) were randomly allocated to 5 groups (3 replicates in each group and 10 piglets per replicate) and fed with basal diets supplemented with 0, 50, 100, 200 mg/kg Zn as Zn-Gly or 3 000 mg/kg Zn as zinc oxide.

选用150头28日龄的杜×长×大三元杂交断奶仔猪，分成5组（每组3个重复，每个重复10头），对照组饲喂基础日粮，试验组饲喂分别在基础日粮中添加50、100和200 mg/kg甘氨酸锌和3 000 mg/kg氧化锌的日粮。

The addition of yeast extract for nitrogen source can increase content of ergothioneine (824.8 mg/ml dw);Fermentation of Pleurotus eryngii was polished buckwheat as medium, moisture content at 45%, temperature at 30℃and cubation 17 days could get high content of ergothioneine (580.6 mg/kg dw), and biomass was 163.4 mg/g dw, yeast extract as nitrogen source (651.6 mg/kg dw), Fructose as carbon source (721.4 mg/kg dw).

在碳源方面，添加果醣能提高麦角硫因含量为721.4 mg/kg dw。

Results 6.3 mg/kg diazinon decreased the number of IgMplaque forming cell,blood serum erythrocytolysin level and DTH to SRBC.12.5,25 mg/kg dizainon could decrease the relative weight of thymus at about 20%,the number of IgMplaque forming cell at about 39% and 52%,the blood serum erythrocytolysin level at about 15% and 20%,the DTH to SRBC at about 33% and 35%,the ability of T lymphocyte proliferation at about 27% and 35%,the activity of NK cell at about 24% and 28%,respectively.

结果 敌匹硫磷染毒4周后，6.3 mg／kg组小鼠的体液免疫功能和迟发型变态反应受到抑制；12.5，25 mg/kg组小鼠的胸腺脏器系数均降低20％，抗体生成细胞数分别降低39%和52％，血清溶血素水平分别降低15％和20％，迟发型变态反应水平分别降低33％和35％，淋巴细胞增殖能力分别下降27％和35％，NK细胞活性分别下降24％和28％。

Totally 50 male Kunming mice were recruited and randomized into 5 groups: normal control group, model group,positive control group, 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside 0.03 g/kg group (low-dose group), and 2,3,5,4 '-tetrahydroxystilbene-2-O-β- D-glucoside 0.1 g/kg group(high-dose group).2,3,5,4'-tetrahydroxystil bene-2-O-β-D-glucoside was an effective component extracted from Chinese herb tuber fleeceflower root in the Department of Pharmacology, Xuanwu Hospital of Capital University of Medical Sciences.

选取雄性昆明小鼠50只，随机分为5组：即正常组、模型组、阳性对照组、二苯乙烯苷0.03g/kg组、二苯乙烯苷0.1g/kg组。

With gramineae F. arundinacea of cool-season turf grass as tested material, the experiment of split-split plot design with was adopted and three kinds of treatments were set up, including 40, 50, 80 kg/hm^2 of P application, 60, 80, 140 kg/hm^2 of K application and 0, 250, 500 mg/L of paclobutrazol application resp. to conduct variance analysis on the density and plant height of F.

方法］以禾本科冷季型草坪草高羊茅为试材，采用裂裂区试验设计，分别设施磷肥40、50、80 kg/hm^2、钾肥60、80、140 kg/hm^2、多效唑0、250、500 mg/L，对处理后高羊茅的密度和株高进行方差分析，并用目测法定量描述其景观品质。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。