查询词典 keyhole

Sensitized systematically with keyhole limpet hemocyanin, the animals were inoculated with the same antigen through cochlea basal turn into the labyrinth.

材料与方法选健康成年雌性SD大鼠 2 0只(购于同济医科大学实验中心动物学部)，体重 180～ 2 0 0g ，分为健康对照组(n =5)和实验组(n =15)。

Methods PIWIL polypeptide was synthesized and conjugated to Keyhole limpet hemocyanin as an immunogen. Then PIWIL-KLH conjugations were injected into rabbits subcutaneously to produce polyclonal antibodies.

合成特异性PIWIL多肽，以马来酰胺活化匙孔血蓝蛋白作为载体构建多肽免疫原，通过致敏大白兔，制备兔抗人PIWIL多克隆抗体，用亲和层析法纯化抗体。

Methods ①Human peptides of CYP450 enzymes and their proteinic sequence were analyzed by means of bioinformatics, and certain sequences were chosen and synthesized according to four indexes (Hydrophilicity Plot, Flexible Regions, Antigenic Index and Surface Probability Plot) after homology analysis by software Blast. The synthesized peptides were conjugated to keyhole limpet hemocyanin to increase antigenicity.

①利用生物信息学方法分析CYP1A2、CYP3A4及CYP4A11蛋白的序列，利用DNAstar软件中蛋白质的亲水性结构、柔韧区、抗原指数及表面概率结构指标选择多肽序列，应用Blast软件对所选序列进行同源性分析并合成多肽，与载体蛋白钥孔戚血蓝素偶联，免疫大耳白兔制备抗CYP1A2、3A4及4A11抗体。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

An emission nebula that contains much dust, the Keyhole Nebula is roughly 9,000 light years distant.

钥匙洞星云是一个包含有大量星际尘埃的辐射星云，距离地球大约9000光年。

The top of the Keyhole Nebula, the mostprominent feature embedded inside Carina, is on the left.

深植于船底座中最显著的结构&锁眼&星云的顶部处在左边。

The top of the Keyhole Nebula, the mostprominent feature embedded inside Carina, is on the left.

深植在船底座星云内部最显著的特征&锁眼&星云的顶端位于图像左侧。

The Keyhole Nebula was recently discovered to contain highly structured clouds of molecular gas.

根据最近的观测发现钥匙洞星云中具有相当高结构性的分子云。

Explanation: The dark dusty Keyhole Nebula gets its name from its unusual shape.

黑暗而且多尘埃的钥匙洞星云，是因他的特殊形状而命名的。

The Keyhole Nebula, visible left the center , houses several of the most massive stars known and has also changed its appearance.

图像中心左边是锁眼星云，拥有几个已知的最巨大的恒星，也在一直改变着星云外观。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。