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In conclusion, a comprehensive analysis of water lilies based on morphological and molecular data was performed, showing the phylogenetic relationships among the 10 genera and providing new evidence for classification at family level. The specific and infraspecific relationships within the genus Nymphaea were revealed using the ITS sequences, providing a new approach to germplasm identification and phylogenetic analysis of water lilies. The investigation of invasive fanwort in China may also provide a sound basis of its ecological studies.

总之，本文以国内外长期研究为基础进行了综合定量的形态学与分子序列分析，明确了10个属的系统位置，为科的划分提供了新的依据；通过运用ITS序列研究睡莲属的种及种下单位之间的亲缘关系，为睡莲类植物的种质资源鉴定提供了新的工具；对水盾草进行了初步的调查分析，为今后深入开展其生态学研究打下了良好的基础。

This text had explored four kinds of methods to extract DNA template rapidly and conveniently from 12 kinds of Harmonia axyridis Pallas with different flecks and groundings on their back, followed by PCR and sequencing until the whole ITS-2 sequence.

本试验采用了四种DNA提取方法，提取了12种不同色斑型异色瓢虫的DNA，对异色瓢虫的rDNA-ITS 2经过PCR扩增和测序，得到完整的ITS-2序列。

By analysing the feature of ITS sequence of monocots with reticulate vein,the differentiation and variation characters of ITS sequences in the families,genera and species studied were discussed.

并通过分析单子叶网状叶脉植物和菝葜科部分种类的ITS序列特点，研究了该片段在科、属、种间存在的关系和变异特点。

Arundinacea in vitro. After cultivation on PDA at 26℃ under continuous fluorescent light for 6 days, the colonies were black green and the hyphae were septate. Spore size was 30.6~17.0 μm×10.9~13.6 μm, mostly with four-septa. The central cell was dark and the third cell from the base was the largest. The DNA sequence of ribosomal ITS of the isolates was found to match 100% with an ITS sequence of Curvularia affinis in GenBank. This is the first report of leaf spot caused by C. affinis on F.

结果表明：该病原菌能侵染高羊茅，在26℃和光照下，在PDA培养基上培养6d后菌落呈墨绿色，菌丝有隔，分生孢子有4个横隔膜，平均大小为30.6~17.0μm×10.9~13.6μm，中部3胞暗色，第3个细胞基部膨大；其核糖体DNA-ITS序列分析表明，该菌与GenBank中近缘弯孢的同源性是100%；结合形态学特征和致病性测定，认为该菌为C。

The morphological characteristic showed that the colonies were black green and the hyphae were septum, brown, Spore size was 11.07×25.18μm, mostly ellipse, with three septate , two central cells were larger than both terminal cells , brown to dark brown , terminal cells subhyaline to light Brown after cultured on PDA medium at 5d. The DNA sequence of ribosomal ITS of the isolates was found to match 99% with an ITS sequence of Curvularia verruculosus in GenBank.

为致病菌。该菌在PDA培养基上培养5d后，菌落呈墨绿色，菌丝褐色，有隔，分枝，分生孢子平均大小为11.07×25.18μm，褐色至暗褐色，椭圆形，具有3个隔膜，主隔膜明显加粗，中间两细胞较大。rDNA-ITS测序结果与GeneBank中的序列进行同源性比较发现菌株与Curvularia verruculosus的同源性高达99％。

With the 39 species nrDNA ITS sequence from Genbank and by using nrDNA ITS sequence information of our study 31 species, a phylogenetic analysis was carried out on 70 species of Paphiopedilum, and then the results have been analyzed with the results from RAPD molecular marker of 30 species, indicated that, we supported Kirasawa's system proposed in 1982 on Paphiopedilum that this genus can be divided into six subgenera.

基于 ITS 序列的数据对兜兰属70个种的研究，结合 RAPD 对于中国兜兰30个种的研究结果进行分析，支持唐沢耕司（1982）所提出的将兜兰属分为6个亚属的观点，其中产于中国的兜兰属植物可以划分为5个亚属；认为巧花兜兰不能并入海伦兜兰中，最多可以作为其变种处理，或者独立成种，隶属于兜兰亚属的 Paphiopedilum 兜兰组；不支持将文山兜兰并入巨瓣兜兰或者同色兜兰中，可以作为是其变种处理，隶属于 Concoloria 组。

Thereinto, vehicle measurement is core and base of ITS.

辆的检测、车辆流量统计子系统、红绿灯的优化控制子系统，其中车辆的检测是ITS的核心部分，是ITS的基础。

Parvifolia was 642bp by cloning PCR production and sequencing, and its phylogenetic information might facilitate to provide the direct molecular evidence to clarify its origion and evolution for Buxus sinica var. parvifolia.

珍珠黄杨ITS片段克隆测序后获得的序列长度为642bp，其系统学信息将为珍珠黄杨的起源进化提供有力的分子水平证据。

By using the traditional taxonomic methods, electronic microscope examination, 18S rDNA, ITS sequences and phylogenetic analysis, an endophytic fungus HCCB1621 isolated from the cephalotaxus sinensi was identified as Trichoderma viride Pers.ex Fr. Its anti-tumor activities were studied as well.

利用传统分类学方法、电子显微镜观察、18S rDNA和ITS序列的测定以及系统发育分析对一株分离自粗榧抗肿瘤活性内生真菌HCCB1621进行分类鉴定，并对其产物抗肿瘤活性进行了初步研究。

Internal transcribed sequences of ribosomal DNA of 36 species wereanalyzed to reconstruct the phologeny of Heracleum and its related genera using themaximum parsimony, neighboring-joint and maximum likelihood methods inPAUP4.0b10, respectively.

选取独活属7种植物测取核rDNA中的ITS基因序列，结合GenBank已有的本属5种和邻近属24种植物的ITS序列，用PAUP4.0b10软件中的最大简约法、邻接法和最大似然法分别进行分析，从而对独活属及其近属进行了系统发育重建。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。