查询词典 isolation

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

In 1854, U.S. Commodore Matthew Perry used the threat of force to convince Japanese officials to sign a treaty which effectively ended the long running regime of sakoku , or isolation, that Japan's shoguns had established throughout most of the country for some 200 years.

1854年美国海军准将佩里使用军事力量迫使日本签订一项条约，有效地结束了长期的闭关锁国政策，日本的幕府在国家大部分地区已经持续了200年左右。

Methods The methods of FDA BAM 8th chapter 10 and chapter 11 were used for Listeria monoytogenes isolation and serotyping.

采用美国食品药品管理局颁布的检测方法对单核细胞增生李斯特菌进行分离和血清分型。

The results also indicated that genetic differentiation among populations was large, mainly attributable to geographic isolation due to the discontinuous distribution of the species and high rate of selfing.

在目前分布残缺，群体丢失严重的情况下，紫丁香种质资源保护和利用策略应为采取优先保存尽可能多的群体。

In the 20 days of isolation from the surroundings, I draw an image of mountain on 8.4m long panel (the possible length of my studio) and then erased the drawing. The dust and marks made during the erasing are sealed on the panels.

这隔绝的二十天里，在工作室可容纳的长度内的板面上画山的形象，之后用橡皮擦去，最后将擦拭过程的痕迹及橡皮屑及尘埃等封住在板上。

Isolation will cause a split, and we must bring about a split among the Rightists.

孤立就会起分化，我们必须分化右派。

Greater than -10 mV and shows little inactivation during a 200 ms depolarization. It is completely reprimed at a holding potential of -60 mV, and can be isolated by using a more depolarized h.p.(-40 mV) to inactivate the other two types of calcium currents. 3. Component T can be seen in isolation with weak test pulses.

在测试电位大于-10mV时，L电流被产生并不会在200ms除极化的过程中被抑制。L电流在-60mV时被可被重新发起，并可在-40mV时以抑止其它两种钙离子电流被隔离。

Greater than -10 mV and shows little inactivation during a 200 ms depolarization. It is completely reprimed at a holding potential of -60 mV, and can be isolated by using a more depolarized h.p.(-40 mV) to inactivate the other two types of calcium currents. 3. Component T can be seen in isolation with weak test pulses.

在测试电位大於-10mV时，L电流被产生并不会在200ms除极化的过程中被抑制。L电流在-60mV时被可被重新发起，并可在-40mV时以抑止其它两种钙离子电流被隔离。

After technical transformation, we used the rectifier with high-frequency switch to replace the rectifier with controllable silicon, the JKQ-2000B central controller to replace the PLC, and new WDCX-2G computer-aided control isolation and grounding failure detection apparatus to repla.

技术改造选用了高频开关整流器代替可控硅整流装置，选用JKQ-2000B型中央监控器代替PLC可编程控制器，选用WDCX-2G新型微机直流系统绝缘在线监测及接地故障定位装置代替老型号的绝缘监测装置。

Binocular attention induced by the disparate contours and the disparate isolation areas captured region and background can facilitate and stablize local rematching.

由视差轮廓引导的双眼注意以及在被捕获区域和背景之间的视差隔离区对局域重新匹配起了促进和稳定的作用。

You can do some assertiveness training.

你可以进行一些自信训练。

We were well on the path to making a rear-wheel-drive global platform," says Mays."

我们正致力于建立一个后轮驱动的平台，"Mays这样说道。"