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Subsequently, the performance of the Pastorex Staph-Plus agglutination test with presumptive MRSA isolates taken directly from chromogenic agars was compared to that of the Pastorex Staph-Plus agglutination test with isolates from blood agar subcultures (conventional_Pastorex agglutination).

随后,我们采用Pastorex Staph-Plus乳胶试剂对显色培养基上被推断为MRSA的菌落进行直接乳胶凝集试验,同时采用接种在血琼脂上进行传代的纯培养菌落进行经典的乳胶凝集试验与之进行比较。

A total of 200 strains (z50% gentamicinresistant) from the United States were used for the cross-resistance study including Staphylococcus aureus (110), coagulase-negative staphylococci (CoNS; 50), Pseudomonas aeruginosa (10), Escherichia coli (20), and other Enterobacteriaceae (10) tested against TAO, bacitracin, polymyxin B, neomycin, amikacin, gentamicin, streptomycin, tobramycin, and mupirocin. Fifty gentamicin-resistant isolates from each year (1997–2002) were used to determine the activity of TAO over time. Baseline resistance rates of TAO among 300 Australian isolates (AGARS Program, 2002–2003) were also studied.

来至美国的200株菌株( z50 %对庆大霉素有耐药性)用于交叉耐药性研究,其中包括金黄色葡萄球菌( 110株),凝固酶阴性葡萄球菌( CoNS ; 50株),铜绿假单胞菌( 10株),大肠埃希氏菌( 20 株)和其他肠杆菌属菌( 10株),检测这些菌对于以下抗生素的耐药性,抗生素为TAO,杆菌肽,多粘菌素B ,新霉素,丁胺卡那霉素,庆大霉素,链霉素,妥布霉素,莫匹罗星。1997到2002年期间每年分离出五十株对庆大霉素有抗药性菌株来确定随时间的变化TAO对其的活性是否有所改变。300株澳大利亚分离的菌株(AGARS Program, 2002–2003)也对TAO的耐药性基准进行了研究。

Subsequently, the performance of the Pastorex Staph-Plus agglutination test with presumptive MRSA isolates taken directly from chromogenic agars was compared to that of the Pastorex Staph-Plus agglutination test with isolates from blood agar subcultures (conventional_Pastorex agglutination).

紧接着,使用Pastorex Staph-Plus葡萄球凝集试剂检测直接从显色平板上挑取的疑似MRSA菌落(称之为直接Pastorex 凝集),并与次培养到血平板上的菌落的Pastorex Staph-Plus凝集试验(称之为传统Pastorex 凝集)的结果进行比较。

The fertile ratio of isolates from Jiangsu was 22.77%on average. Among them only 23 crosses produced ascospores and the ratio was 7.1%. But the fertile ratio showed great variation annually, 26.61%in 1997, 8.26%in 1998 and 33.64%in 1999 respectively. In addition, the fertile ratio of tested isolates collected from different areas was also dissimiar., 26.15%and 25.42%from Tongzhou and Ganyu, and only 15.38%from Yixing.

不同年份、不同地区采集的稻瘟病菌菌株的性亲和力和交配型有较大的差异,三年的交配率分别为26.61%、8.26%和33.64%;通州地区和赣榆地区菌株的交配率相对较高,分别为26.15%和25.42%,宜兴地区菌株的交配率较低,只有15.38%。

The ELISA assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of Aspergllius, while the other assay could only detect Aspergillus fumigatus of both clinical and environmental isolates.And no cross reaction with the cell culture of Penialllium marneffei and Candidas was observed with the two methods.

用mAbs-1建立的双抗体夹心ELISA法可检测19种常见曲霉株培养液;用特异性针对烟曲霉抗原单克降抗体(mAbs-2)建立的双抗体夹心ELISA法可特异性检测临床和环境分离株烟曲霉培养液;与其他曲霉株无交叉反应;2种双抗体夹心ELISA法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

Methods PBMCs from 10 heparinized blood specimens of HIV-1 infected persons were collected to isolate the virus with PBMC cocultivation method. Neuramindase was added to the culture medium to raise the HIV isolation rate. The supernatant of cocultures were detected by p24 antigen capture assay and also IFA test was used to identify the isolates. Cell tropism of the HIV-1 isolates was tested byuse of H9 and MT4 cell lines, respectively.

采集10份福建艾滋病病毒感染者肝素抗凝血,分离外周血单核细胞,与健康人PBMC共培养进行HIV-1的分离,使用含神经氨酸酶的T细胞培养液提高病毒分离率,通过检测P24抗原、间接免疫荧光试验及电镜观察等确定病原分离结果。

The 59 pure cultural isolates, including 3 Fusarium oxysporum f.sp.cucumarinum, 26 other Fusarium isolates and 20 fungi, 6 bacteria, 7 actinomycetes from cucumber rhizosphere, have been detected by PCR-RFLP and Nested-PCR. It shows that PCR-RFLP is special for the pathogen of Cucurbits Fusarium Wilt, and Nested-PCR is special for Fusarium oxysporum f.sp.cucumarinum.

本论文应用PCR-RFLP和巢式PCR程序对3株黄瓜尖镰孢菌(Fusarium oxysporum f.sp.cucumarinum)、26株镰孢属部分真菌及分离自土壤的20株真菌、6株细菌和7株放线菌共59个菌株进行扩增,表明PCR-RFLP程序对瓜类枯萎病菌具有特异性,而巢式PCR对黄瓜尖镰孢菌具有特异性,巢式PCR程序检测黄瓜尖镰孢菌的特异性高于PCR-RFLP程序。

The main purpose of this research is to evaluate a useful biocontrol formulation for producing gas by four strains of gas-producing bacteria, Enterobacter cloacae isolates E006、E010 and Bacillus mycoides isolates CHT2401、CHT2401, to control orange green mold.

本研究主要的目的在於探讨Enterobacter cloacae E006、E010与Bacillus mycoides CHT2401、CHT2402菌株所产生之气体的抑菌效果,进而评估它们於密闭贮藏环境下产气防治柳橙绿霉病害的效果。

The three isolates have four specific replacements in 87 , 88 , 95 , and 119 in S1 hypervariable region, and a unique cleavage site R-X-R-T-G-R in 3' terminal region. These data may be valuable for investigation of molecular basis of the serotyping, tissue tropism and pathogenicity of these virus isolates.

本文的3个分离毒株Q1、J2和T3在S1高变区的87,88,95和119有4个特有的替换,在3'末端有独特的裂解位点R-X5-R-T-G-R,这些结果可能对这些分离毒株的血清型特异序列和组织趋向性和致病性的分子决定区方面的研究有重要的价值。

The three isolates have four specific replacements in 87, 88, 95, and 119 in SI hypervariable region, and a unique cleavage site R-X-R-T-G-R in 3 terminal region. These data may be valuable for investigation of molecular basis of the serotyping, tissue tropism and pathogenicity of these virus isolates.

由于SI蛋白序列99-12川立氨基酸之间的序列可能对病毒的致病性起重要作用,SI蛋白5,高变区(56-69;117-131)与中和表位和血清型密切相关,这些区域在IBV血清型和基因型间起联系作用。

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