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Three infectious bronchitis virus isolates named as Q1, J2 and T3 were isolated from proventricular tissue taken from vaccinated chicken flocks in China The biological characteristics of the three isolates were observed.

从我国接种过传染性支气管炎疫苗的幼鸡群的腺胃组织中分离得到三个传染性支气管炎毒株Q1、J2和T3,并对它们的部分生物学特性进行研究。将病毒通过9-11日龄鸡胚,盲传5-6代。

①The collected clinical Acinetobacter baumannii isolates were more seriously resistable to Ciprofloxacin than to Imipenem;②The antibiotic resistance of clinical Acinetobacter baumannii isolates may be associated with the decline of CarO protein expression;③There is some relationship between CarO protein and ciprofloxacin-resistant Acinetobacter baumannii strains,it proves that CarO protein supports bacteria on multi-drug resistance;④CarO protein may be related to cell signalling;⑤The difference in CarO protein functional sites and structure between the sensitive strains and the resistant strains may be related to bacterial resistance.

①收集的临床鲍曼不动杆菌对环丙沙星的耐药情况较亚胺培南严重;②CarO蛋白的表达下调可能与临床鲍曼不动杆菌的耐药性相关;③CarO蛋白与鲍曼不动杆菌耐环丙沙星有一定相关性,佐证了CarO蛋白可能与细菌多重耐药有关;④CarO蛋白可能与细胞信号传导有关;⑤敏感菌株与耐药菌株的CarO蛋白功能位点和内部结构有差异,可能与细菌耐药有关。

According to geminivirus taxonomic criteria, we named these viruse isolates as Stachytarpheta leaf curl virus. Hn5-1, Hn5-4 and Hn6-l shared more than 95% identity to each other, indicating they are different isolates of StaLCV.

根据双生病毒分类命名原则,Hn5-1、Hn5-4和Hn6-1为同种病毒的不同分离物,我们将这些病毒分离物命名为假马鞭曲叶病毒(Stachytarpheta leaf curl virus, StaLCV)。

Fungal isolates which belong to 28 or more genera, were identified by standard blotter method. The most common fungi isolated were Aspergillus (11.66% of all fungal isolates), Tilletia horrida (10.54%), Fusarium (9.36%), Rhizoctonia solani (8.07%), Penicillium (7.54%), Alternaria (4.95%), Rhizopus (4.59%), Ustilagiiwidea virens (4.18%), Sclerotium (4.12%), Mucor (3.36%) species.

这些真菌菌株分属于至少27个属,其中以Aspergillus(占总菌株的11.66%)、Tilletia horrida (10.54%)、Fusarium (9.36%)、Rhizoctonia solani (8.07%)、Penicillium (7.54%)、Alternaria (4.95%)、Rhizopus (4.59%)、Ustilaginoidea virens (4.18%)、Sclerotium (4.12%)、Mucor (3.36%)这10个属种为优势菌群,合计占总菌株数的68.37%。

In order to analyse the relationship between several Trichinella isolates from different areas in China,we amplified the COI gene from these isolates and obtained their sequences.

为了了解我国几个不同地区旋毛虫之间的种属关系,我们对这些地区的旋毛虫进行了线粒体细胞色素氧化酶亚单位I基因的扩增,并得到了正确的COI基因。

Results By MSHA/MRHA, the fimbriae of 4 of the 5 isolates were identified as type Ⅲ, and the rest one as undefined type. However, by PCR, all the fimbriae of 5 isolates were identified as type Ⅲ.

结果 MSHA/MRHA方法鉴定其中的4株菌具有Ⅲ型菌毛,另外1株无法鉴别,而PCR方法鉴定5株菌均具有Ⅲ型菌毛。

We use whole soya beans and not protein isolates because soy protein isolates are one of the most refined soy products available.

我们选择完整大豆而不是分离蛋白制作豆奶,因为分离大豆蛋白是精加工提炼过的,在提取的过程中大部分的营养元素已经丢失。

Three hundred and twenty-five isolates of Magnaporthe grisea collected from rice plants in Wujiang, Yixing, Tongzhou, Gaoyou and Ganyu of Jiangsu province from 1997~1999 were tested for mating type and fertility with standard tester strains. Seventy-four tested isolates produced perithecia after mated with tester strains, in which, 48 were MAT1-1 and 26 were MAT1-2 mating type.

用标准菌株对1997~1999年在江苏省吴江市、宜兴市、通州市、高邮市和赣榆县采集的325个稻瘟病菌单孢分离菌株的可育性和交配型进行了测定,结果表明江苏省稻瘟病菌菌株的育性较低,可交配率为22.77%,可育率仅为7.08%。

The three isolates were named A/Bar-headedGoose/Qinghai/F/2006(H5N1), A/Black-headed Gull/Qinghai/3/2006(H5N1) andWhooper Swan/Qinghai/01/2006(H5N1). The research results were, nucleotidesequence analysis revealed that three isolates shared a high homology with each other(99%) except PB1 genes of Bar-headed Goose/Qinghai/F/200 and Whooperswan/Qinghai/01/2006, which shared 94% identity.

通过对3株病毒的全基因组序列分析与进化研究,其分子特征分析结果显示:Whooper swan/Qinghai/01/2006株和Bar-headed Goose/Qinghai/F/2006株的PB1基因的核苷酸序列同源性为94%之外,3株病毒其余基因片段的核苷酸序列同源性为99%。

In the mating test, mycelial protoplast pairs between single spore isolates of different strains formed colonies with clamp connection, while mycelial protoplast pairs between the same single spore isolates formed colonies without clamp connection, suggesting that the two nuclei of the spores possessed identical mating type factors.

在交配试验中,源于不同菌株单孢分离物的菌丝原生质体的配对形成具锁状联合的菌落,而源于同一单孢分离物的菌丝原生质体的配对则形成无锁状联合的菌落,暗示担孢子中的两个核具有相同的交配型。

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