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These results revealed that the prepared serum and relevant assay could be used for serotyping field isolates.

多聚酶链式反应(Polymerase Chain Reaction，PCR)由于具有敏感快速的优点，已被成功应用于许多病原的快速检测和疾病诊断中。

Clinical data were collected on all subjects with IPD, and antibiotic susceptibility testing and serotyping were performed on all available pneumococcal isolates.

所有IPD受试者的临床资料均被收集，对所有可得的分离肺炎球菌菌株均行抗生素药敏试验和血清分型。

In conclusion, we have isolated and identified two strains of Hps, fifteen standard positive sera for serotyping were produced and 96 Hps isolates were serotyped.

总之，本研究分离鉴定了2株副猪嗜血杆菌、制备了15个血清型的副猪嗜血杆菌标准阳性血清及对96株Hps进行了血清型分型。

The serotyping of ZYMV isolates from China indicated that Serotype XI wasdominant type while the serotype I dominanted in most regions of the world wasfound at very low proportions in China.

ZYMV中国分离物的血清型分型结果表明：我国ZYMV的分离物的血清型主要为XⅠ型，占极大多数（80%以上）：世界其它地区大量发生的血清型Ⅰ，在我国则很少发生。

Fluorometric assay for norfloxacin uptake by bacteria showed the concentration of fluroquinolone-resistant isolates was same as that of the sensitives.

使用荧光分光光度计对细菌体内的诺氟沙星含量进行测定，结果发现耐药菌内诺氟沙星的聚积浓度与敏感菌相同。

The genetic study suggested that the carbendazim-resistance in these mutants could be steadily inherited by asexual and selfed reproduction, and the resistance was controlled by the same single major gene both in laboratory mutants and field resistant isolates, the different levels of resistance or the same resistant level in different strains maybe conferred by mutations at different sites or one site with different allelic mutations. The gene compensates for the sensitivity to MDPC was allelic to that governs the carbendazim resistance, but the mutation for increasing sensitivity to MDPC in this gene could get the pathogen highly resistant to carbendazim.

抗药性遗传研究表明，在所研究的抗药突变体中，抗药性在自交和无性繁殖后代中能稳定遗传；室內抗药突变体和田间抗药菌株对多菌灵的抗药性由同一个主效基因控制，但它们发生突变的位点不同或者同一碱基位点发生了不同的突变；对MDPC的敏感性也是由单个基因控制的，该基因与控制多菌灵抗性的基因是等位基因，当该基因发生对MDPC的敏感性增加的突变时会使病菌对多菌灵产生高水平抗性。

Methods: A randomized, single blind controlled clinical design was used to accomplish the series clinical trials included 123 cases. The troop of gatifloxation injection with sodium chloride included 20 cases in gatifloxation group and 23 cases in ciprofloxation group; The troop of gatifloxation injection with glucose included 21 cases in gatifloxation group and 19 cases in levofloxacin group; The troop of gatifloxation pivoxil included 19 cases in gatifloxation group and 21 cases in ciprofloxation group. The antibacterial activity of clinical isolates were detected by scrips which contain gatifloxation(5ug/L), levofloxacin (5ug/L), ciprofloxation (5ug/L), spafloxation(5ug/L), cefotaxime(30ug/L), penicillin(10U/L) respectively. The MICs of sixkinds of antibiotics were detected by 2-fold agar dilution method. Result: The common data of patients in the two groups of 3 troops were similiar.

采用区组分层均衡随机单盲试验设计，分别完成甲磺酸加替沙星氯化钠注射液组试验药20例及对照药23例、甲磺酸加替沙星葡萄糖注射液组试验药21例及对照药19例、甲磺酸加替沙星片剂组试验药19例及对照药21例共123例感染患者的临床试验；用K-B法测定临床分离致病菌对加替沙星(5ug／L)、左氧氟沙星(5ug／L)、环丙沙星(5ug/L)、司帕沙星(5ug/L)、头孢噻肟(30ug／L)、青霉素(10U／L)纸片的敏感性，再用按美国国家实验室标准委员会(National Committee for Clinical Laboratory Standards，NCCLS)推荐的琼脂平皿对倍稀释法测定临床分离菌株对加替沙星、左氧氟沙星、环丙沙星、司帕沙星、头孢噻肟、青霉素的最低抑菌浓度(Minimum Inhibitory Concentration，MIC)以了解其体外抗菌活性。

Objective To acquire enough envelope glycoproteins so as to facilitate a further study of the structure and function of envelope glycoproteins from various kinds of virus isolates. Methods Two envelope glycoprotein gene fragments were cloned from the recombinant plasmid pHXB2 containing the human immunodeficiency virus 1(HIV-1) proviral genome of HXB2 isolate.

目的 获得足够量的膜糖蛋白，以便于对不同HIV分离株膜糖蛋白的结构与功能进行进步的研究方法从人免疫缺陷病毒1(HIV-1)HXB2分离株原病毒基因组的重组质粒pHXB2中克隆了两段膜糖蛋白基因片段。

On the basis of phylogenetic analyses, three isolates, strain 117,43401 and 12202 were subject to a polyphasic taxonomic study.

实验结果显示，酸性土壤中的嗜酸稀有放线菌具有较丰富的种属多样性，其中诺卡氏菌属是一优势菌群。

By first performing an exercise that isolates and tires the pectorals before immediately moving on to the main exercise.

先执行一项工作，隔离和轮胎的胸肌之前就立即移动的主要工作。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。