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ABSTRACT Objective To investigate the prevalence and location of qnrA gene in clinical isolates of Enterobacter cloacae and its function in the development of quinolone resistance.

目的 了解深圳地区阴沟肠杆菌中qnrA基因的流行情况、基因定位及其介导喹诺酮耐药性产生的机制。

ABSTRACT Objective To investigate the prevalence and location of qnrA gene in clinical isolates of Enterobacter cloacae and its function in the development of quinolone resistance. Method Forty?

目的 了解深圳地区阴沟肠杆菌中qnrA基因的流行情况、基因定位及其介导喹诺酮耐药性产生的机制。

The primers and the detection programs have been used to detect the pathogens in the inoculated-diseased cucumber plants .The details are reported as follows: 1. By using the primers ITS1/ITS4, only Fusarium isolates could be amplified to produce a special fragment of 750bp. This product generated 480bp, 200bp and 100bp fragments after digested by restriction enzyme HinfⅠonly from the pathogen of Cucurbits Fusarium Wilt.

引物ITS1/ITS4只在镰孢属菌株上扩增到一条750bp分子片段,其他供试菌株无扩增条带产生,扩增产物经HinfⅠ酶解后,只有瓜类尖镰孢菌产生480bp、200bp和100bp 3条带,说明该PCR-RFLP程序对瓜类尖镰孢菌具有特异性;巢式PCR引物BIK1/BIK4和BIK2/BIK3只有在黄瓜尖镰孢菌菌株上扩增到一条800bp分子片段,其他菌株均无扩增条带产生,说明该巢式PCR程序对黄瓜尖镰孢菌具有特异性。

Dendrogram of UPGMA cluster analysis revealed that 18 isolates of U.

说明分子多样性同地理来源之间具有一定的相关性,但与寄主来源无关。

In this study, we found the Epichlo-Roegneria symbionts were widespread in the middle and lower basin of Yangtze River, and significant differences in the morphological characters between these fungal isolates from China and other Epichlo endophytes reported previously.

研究发现我国的Epichlo-Roegneria组合在长江中下游地区分布比较广,资源丰富,而且这些真菌的形态学特征显示,这些菌株与前人报道的有明显的差别。

Twenty-nine plants with stroma and 87 plants without stroma collected were detected to be all infected by endophytes, and 20 fungal isolates were isolated and selected from these samples.

从29株着生子座的植株和87株不着生子座的植株中全部检出了内生真菌,从中分离得到了20个菌株。

Previous studies indicate that the presence of speA?Aspec,or speF genes is not implicated in any particular clinical syndrome in patients with invasive GAS disease. Yet, high-level protease activity of the isolates are significantly associated with the clinical signs of STSS and with mortality.

研究显示带有最严重的A群链球菌感染的病人或有SPE A、C或F的基因,但无法与任何特殊的临床症状产生相关性,反而从病人分离出来的菌株常带有高量蛋白脢的活性,且与STSS的临床症状及死亡率有关。

Previous studies indicate that the presence of speA, speC, or speF genes is not implicated in any particular clinical syndrome in patients with invasive GAS disease. Yet, high-level protease activity of the isolates are significantly associated with the clinical signs of STSS and with mortality.

研究显示带有严重的A群链球菌感染的病人或有SPE A、C或F的基因,但无法与任何特殊的临床症状产生相关性,反而从病人分离出来的菌株常带有高量蛋白脢的活性,且与STSS的临床症状及死亡率有关。

The progress levels of symptoms after pathogen infection showed that interaction of gray leaf spot pathogen and host has differentiation or polymorphism. The progress levels of symptoms is different.The aggressively has polymorphism. The pathogenicity of different isolates in same cultivar has polymorphism, or the host reaction type of same isolate in different cultivars has polymorphism.The host reaction type has differentiation. There are seven types of host reaction in cultivar and inbred after infected by GLS pathogen:Rectangular lesion without chlorotic halo, Rectangular lesion with chlorotic halo,Irregular lesion without chlorotic halo,Irregular lesion with chlorotic halo, Spot lesion with chlorotic halo,Rectangular and irregular lesionand Rectangular and spot lesion. The host reaction type is coalesced lesion when disease severely. Frequency of each reaction types is different.

通过对玉米灰斑病菌侵染后的显症过程的系统观察,可在组织水平上说明该病菌与寄主互作反应存在分化或多样性:玉米灰斑病菌不同菌株侵染后显症过程表现出明显的差异;玉米灰斑病菌在侵袭力上存在多样性,菌株间对同一种品种的致病性表现多样化,或者同一菌株对不同品种侵染引起的寄主反应表现为多样性;病斑反应型的分化,玉米灰斑病菌侵染玉米品种或自交系后,主要有7种病斑反应类型,即RH型、RN型、IRH型(不规则形具褪绿晕圈病斑)、IRN型(不规则形无褪绿晕圈病斑)、SH型、RI型(长矩形与不规则形混合病斑)和RS型(长矩形与斑点形混合病斑),若发病严重,病斑连成片,而且这些反应类型出现的频率也不同。

However, for other products such as topicals, inhalants or nasal solutions where there is a major concern for microbiological contamination, isolates from plate counts, as well as enrichment testing, should be identified.

但是,对其他的产品,如局部用药,吸入剂或者鼻滴液,这些产品的微生物污染成为这类型产品的主要问题,那么总菌数检测中分离到的细菌,和富集检测中的菌落都应该鉴别。

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