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Methods resistance of Salmonella isolates were detected by drug susceptibility tests with KirbyBauer diffusion method. PCR was designed to detect tetA, tetB, tetC, tetD, tetE, tetG and tetK. The amplified products were sequenced and alignment analysis was performed.

用KB法测定分离株对四环素等21种抗生素的耐药情况，用PCR方法检测四环素耐药基因tetA、tetB、tetC、tetD、tetE、tetG及tetK，并对扩增产物进行测序。

Methods The resistance of Salmonella isolates were detected by drug susceptibility tests with KirbyBauer diffusion method. PCR was designed to detect tetA, tetB, tetC, tetD, tetE, tetG and tetK.

用KB法测定分离株对四环素等21种抗生素的耐药情况，用PCR方法检测四环素耐药基因tetA、tetB、tetC、tetD、tetE、tetG及tetK，并对扩增产物进行测序。

Some 90% of the tested 110 extracts appeared antitumor activity and 13 strains of the 55 isolates showed higher inhibitory capacitieseffective concerntrations ≤16μg/ml）, which may imply a possiblility in their further medical application;whereas the percentage of positive extracts was higher from the thalli than that from the supernatants and of actived strains was higher in the bacteria than that in the molds according to this study.

结果表明，55株微生物发酵样品共110个提取物中，90％样品表现出细胞毒活性；其中13株微生物的活性较强（提取物有效抑制浓度≤16μg/ml），具有较好的开发应用前景。同时还发现，菌体中检测到的活性菌株数大于发酵液中检测到的活性菌株数，细菌的筛选得率高于霉菌的筛选得率。

Hemagglutinin thermostability and hemagglutination spectrum tests: Large variations in hemagglutinin thermostability of 29 SIV isolates from various provinces were detected, with 22 being thermolabile, 2 moderately thermostabile and 5 thermstabile. Two human Influenza Virus strains were either thermal stable or non-stable.

血凝素热稳定性和血凝谱测定结果证实，不同地区SIV分离株的血凝素热稳定性差异较大；22株表现为热不稳定性，2株为中等热稳定型，5株为热稳定型；2株人流感病毒分别表现为热不稳定型和热稳定型。

Hemagglutinin thermostability and hemagglutination spectrtum tests: Large variations in hemegglutinin thermostability of 8 different regions of SIV isolates were detected, with SWTJ/01/04, SWHN/01/06, SWCB/01/04 and SWAH/01/06 being thermstabile and other 4 thermolabile.

血凝素热稳定性和血凝素凝集谱分析：不同地区SIV分离株的血凝素热稳定性差异较大，其中SWTJ／01／04、SWHN／01／06、SWCB／01／04、SWAH／01／06分离株表现为热稳定型，其他4株表现为热不稳定型。

The xylanases produced by the two isolates were alkaliphilic and thermostable enzymes which would be useful in biotechnological fields.

由XJU-1和XJU-80产生的木聚糖酶是生化领域有用的嗜碱耐热酶。

We detected the distribution of lipases in 35 Aeromonas isolates by PCR andphenotypic test using tributyrin as substrate. The positive rates were 100% and 83%,respectively. The lipase activity or associated genes could be detected in the two avirulentstrains, A. hydrophila W1 and A. hydrophila 4332. It suggested that lipase might not be animportant factor in pathogenic mechanism of A.

对本实验室保存的35株气单胞菌分离株进行了脂酶的表型检测和PCR检测，结果分别为83％和100％，特别是嗜水气单胞菌无毒株W1和Ah4332也能检测到脂酶活性或基因，表明脂酶在该菌的致病机制中不是决定性因子。

The gene segment in 18S ribosome RNA gene of four Trichinella isolates were cloned and sequenced.

方法通过RT-PCR方法，克隆4个不同旋毛虫隔离种的18SrRNA基因片段，并进行比较分析。

Forty-nine isolates of Trichoderma were collected and isolated from spawn and fruiting body of edible fungi.

从木霉污染的食用菌菌筒和子实体中分离纯化了49株木霉菌株。

Methods Three primers, including4,5 and M13 core sequence (5'-GAGGGTG～GCGGTTCT-3'), were used to distinguish variations among 20 clinical isolates of T. rubrum and Trichophyton mentagrophytes.

方法采用寡核苷酸重复序列4，5及M13中心序列(5'-GAGGGTGGCGGTTCT-3')等3种引物，扩增 20株红色毛癣菌和须癣毛癣菌临床分离株的DNA，观察产物电泳带型的差异。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。