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Enteritidis were obtained from poultry and its meat products.The antibiotic resistance of food isolates was higher than clinical isolates. The drug resistance spectrum of diarrhea patients isolates in 2006 was different from that in 2007.76 strains including 13 food-borne strains belong to 21 PFGE pattern combinations,and their genetic similarly was higher than 80%.

包括13株食品株在内共76株肠炎菌株可分为21种PFGE带型，遗传同源性接近80%，优势带型依次为3型（34株）、1型（12株）、2型（5株）和5型（5株），除2型外均发现有与腹泻株同型的食源株。2006和2007年的PFGE1/2型病例分别为4例和12例。

Rep-PCR method was used to conduct cluster analysis to the 39 bacterial isolates. rep-PCR analysis revealed that the 39 bacterial isolates could be divided into 12 clusters at the 0.9 dissimilarity percentage level. The 39 bacterial isolates had their own specific band patterns, showing obvious genomic heterogenicity and rich genotypic polymorphism genetic diversity between each isolate.

采用rep-PCR技术对39个细菌分离株进行聚类分析，在rep-PCR图谱的相异百分数为0.9的水平上被聚类为12个类群，39个分离株各自具有独特的带型，说明各细菌分离株的基因组之间存在显著异质性，表现出丰富的基因型多态性。

Pathogenieity and virulence of the isolates were assessed; Soluble mycoprotein and esterase etc isoenzynie patterns of 43 isolates were analyzed; Genetic diversity among 40 isolates of Curvularia lunata was evaluated by random amplified polymorphic DNA fingerprint.

通过对43个新月弯孢分离物的3种同工酶及可溶性蛋白分析和40个分离物的RAPD指纹图谱分析，表明玉米弯孢菌叶斑病病原群体存在遗传多样性。

Methods Targeting the most common mutation types at codon 526 and codon 531 of Rifampiein Resistance Determining Region of rpoB gene from Mycobacterium tuberculosis ,labeled probes （526CAC, 526TAC, 531TCG and 531TTG） were designed to detect 38 Rifampin-resistant clinical isolates with known RRDR sequence, 24 Rifampin-sensitive clinical isolates with wild type sequence of RRDR and 5 nontuberculous mycobacteria isolates, then a method using real-time PCR was established.

方法针对结核分枝杆菌rpoB基因利福平耐药决定区（Rifampicin Resistance Determining Region，RRDR）526密码子和531密码子常见的突变形式设计探针（526CAC，526TAC，531TCG和531TTG），应用已知rpoB基因RRDR区序列的38株利福平耐药临床分离株和24株利福平敏感临床分离株以及5株非结核分枝杆菌菌株建立荧光定量PCR检测基因突变的方法。

To control tobacco black shank disease rationally, reduce resistance, 34 strains of Phytophthora parasitica var. nicotianae were isolated from 6 region of Yunnan province and all isolates were determined their resistance to metalaxyl. The results indicated that of all tested isolates, there were 12 high resistance, 5 middle resistance and 17 low resistance isolates respectively.

为合理防治烟草黑胫病，减少抗药性产生，从云南省6个地州分离烟草黑胫病菌菌株34个，就分离菌株对甲霜灵的抗药性进行了测定，结果表明，供试34个菌株中中高抗菌株12个，中抗菌株比例较高5个，低抗菌株17个。

Two isolates ( BO1218 BO1199)can form perithecia when they were cultured with KA3(MAT1-1) and Z46(MAT1-2).Three isolates ( BO1218 BO1199 and BO1535) can form perithecia when they were cultured with KA3(MAT1-1) and KA9(MAT1-2).These five isolates have MAT1-1 and MAT1-2 which are functional.

其中2个菌株(BO1218、BO1199)与KA3和Z46杂交都形成子囊壳，3个菌株(BO1643、BO1563和BO1535)与KA3和KA9杂交都形成子囊壳，表明这5个菌株中存在MAT1-1和MAT1-2，并且具有完整的功能。

Anguillarum isolates, while 94.3% and 91.9% nucleotide identical to L. pelagius and Photobacterium damselae respectively. MP analysis showed that ayu-H080701 shared 97.6%-98.8 % amino acid sequence identical to L. anguillarum isolates, while lower than 75.6 % to other bacteria. Phylogenetic analysis showed that ayu-H080701 grouped constantly with L. anguillarum isolates. The biochemical, physiological tests and sequence analysis all strongly supported the identification of the pathogen causing ayu vibriosis in Ninghai country, China, as an isolate of L.

PCR扩增检测表明，细菌16S rRNA 基因通用引物和鳗利斯顿氏菌MP基因特异引物均能扩增到预期大小的特异性条带。ayu-H080701与鳗利斯顿氏菌16S rRNA基因核苷酸序列同源性最高，为99.4%～99.5%，与同属的海弧菌和美人鱼发光杆菌分别为94.3%和91.9%；ayu-H080701与鳗利斯顿氏菌MP氨基酸序列同源性高达97.6%～98.8 %，与其它弧菌科成员则低于75.6 %，系统进化树分析也揭示ayu-H080701与鳗利斯顿氏菌进化相关性最高。

Four isolates, SP4, SP5, BEG 167 and BEG 141. were selected from the 17 tested isolates. The effectiveness of the isolates was related to ecological attributes such as the number of propagules formed in the soil.

以宿主植物的生物量、产量及吸磷效应作为筛选高效菌株的主要标准，在所用的17个菌株中，选出SP4、SP5、BEG167和BEG141为高效菌株。

Sequence analysis revealed that nucleotides sequence of 28 kD protein gene varied among different isolates of wheat yellow mosaic virus.28 kD protein genes of the Luotian and Huangchuan isolates comprised 762 nucleotidesand were less 3 nt than others,located at the site of 326,327 and 336 nt,respectively.The different isolates shared homologies ranging from 92.1% to 96.9% in nucleotide sequence and 89.8% to 97.3% in amino acid sequence.

序列分析结果表明，小麦黄花叶病毒不同分离物的28kD蛋白基因的核苷酸序列存在一定的差异：湖北罗田和河南潢川分离物在第326，327和336位较四川雅安、江苏扬州及日本分离物缺少3个核苷酸，前两者核苷酸长度为762nt，后三者为765nt；不同分离物核苷酸序列同源性从92 1%到96 9%，相应的氨基酸序列同源性从89 8%到97 3%。

To control tobacco brown spot disease rationally, reduce resistance to fungicide and reduce pesticide pollution, 96 strains of Alternaria altemata were isolated from 11 region of Yunnan province and all isolates were determined their resistance to dimethachlon. The results indicated that of all tested isolates, there were 28 high resistance, 29 middle resistance and 29 low resistance isolates respectively.

为合理防治烟草赤星病，减少抗药性产生和农药残留，从云南省11个地州分离烟草赤星病菌菌株96株，就分离菌株对菌核净的杭药性进行了测定，结果表明，供试菌株中高抗菌株28个，中抗菌株39个，低抗菌株29个。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。