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The 5〓 component DNA (DNA5) of the two isolates of banana bunchy top virus were cloned and sequenced.

采用特异性引物PCR扩增方法，分别克隆得到了BBTV NS和NSP株系组分5的全基因序列。

In summer 2000, five isolates of infectious bursal disease virus were isolated from field cases in southern Hebei Province.

在2000年夏季，我们自河北省南部地区高死亡率的不同鸡群分离到5株（YN、WA、XT、SH和HD）鸡传染性囊病病原。

All the isolates were first identified by different culture media including Niger seed agar. CACA agar, urease tests, ID32 identification kit, 37℃ growth test, and capability of forming capsule, and then the sequence of the LSUrDNA D1/D2 region were further analyzed.

分别以油菊籽、咖啡酸玉米琼脂、刀豆氨酸－甜菜碱－溴麝香草酚蓝培养基、尿素酶试验、37℃生长试验、ID32生化鉴定板和英膜形成试验等初步鉴定后，再经大亚基核糖体DNA的D1/D2区域序列分析进一步鉴定菌种。

Roots of five species of Calanthe and two species of Phaius from north, midland and south of Taiwan were collected for the isolation of orchid mycorrhizal fungi. Total for 104 isolates of fungi were isolated. They are identified respectively by the traditional method or DNA sequence. Results showed thatTrichoderma and Mucor were most often isolated fungi. The DNA sequence results showed that only Xylaris was most common fungus.

由台湾北、中、南四处地方采集五种原生根节兰及二种原生鹤顶兰，进行此等原生兰花根部菌类分离，共分离出104株代码真菌菌种，分别再以菌落、菌丝和孢子等传统形态特徵鉴定菌种，结果发现以木霉菌及毛霉菌属内之菌株占优势，而利用核酸序列比对之鉴定结果则以炭角菌属内之菌株占多数。

When the cultures of Trichoderma isolates which possess the stronger antagonistic ability to S.sclerotiorum are introduced into the soil, the sclerotia burried in this soil is to decay six months later, the carpogenic germination of sclerotia that has not decayed reduces sharply.

将拮抗作用强的木霉菌株培养物导入土壤后，埋入土壤中的菌核多数将发生腐烂，即使未腐烂的菌核其产子囊盘萌发能力也大大降低。

Twenty-three isolates of gray leaf spot pathogen, Cercospora zeae-maydis, collected from growing area of Northern China were clustered based on their soluble protein and isozyme profiles obtained through native PAGE in order to base physiologically the pathogen differentiation at protein or isozyme level.

利用聚丙烯酰胺凝胶电泳技术，对采自北方玉米主产区的23个玉米灰斑病菌菌株进行可溶性蛋白质和同工酶电泳图谱分析及聚类分析，从蛋白质和酶学的多态性水平上分析玉米灰斑病菌的生理分化特征。

Methods Forty-two Gram-positive coccal isolates were acquired from infection cases in Luoyang, Jiyuan, Xuchang and Nanyang of Henan Province, and were analysed by means of 16S rRNA gene sequencing and Vitec-32 automatic identification system respectively.

对从河南洛阳、济源、许昌、南阳等地送检病猪体内分离到42株革兰阳性球菌分离纯化后，分别用16S rRNA基因序列和Vitek-32全自动细菌鉴定系统对其进行鉴定。

Forty-two Gram-positive coccal isolates were acquired from infection cases in Luoyang, Jiyuan, Xuchang and Nanyang of Henan Province, and were analysed by means of 16S rRNA gene sequencing and Vitek-32 automatic identification system respectively.

将从河南洛阳、济源、许昌、南阳等地送检病猪体内分离的42株革兰阳性球菌纯培养后，分别用16S rRNA基因序列比较和Vitek-32全自动细菌鉴定系统进行鉴定。

The susceptibility rates of cefoperazone/sulbactam in 738 isolates were 94% in gram negative bacilli, and 86% in gram positive cocci.

头孢哌酮－舒巴坦对临床分离的革兰阴性杆菌的敏感性为94%，对革兰阳性球菌的敏感性为86%。

Results Of the 3 960 clinical isolates, 61.8%（2 448/3 960） were gram-negative bacilli and 38.2%（1 512/3 960） were gram-positive cocci.

结果 2005年我院共分离细菌3 960株，其中革兰阴性杆菌2 448株，占61.8%，革兰阳性球菌1 512株，占38.2%。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。