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The relationships between germination of the teliospores of Tilletia conroversa Kühn and soil moisture were studied and it was found that the teliospores of TCK could germinate when the soil mass moisture content reached between 1%-28%(relative moisture capacity 3.75%-100%). The suitable moisture for teliospore germination was between 10%-25% of mass moisture capacity or 17.85%-89.3% of relative moisture capacity. The optimal soil relative moisture capacity was between 65%-75%. There was no difference among most TCK isolates tested at the same suitable soil moisture.

通过土壤湿度对小麦矮腥黑穗病菌( Tilletiacontronversa Kühn，TCK)萌发率的影响试验研究表明，其冬孢子在土壤质量含水量为1%～28%(相对含水量3.57%～100%)范围内均可萌发，其适宜萌发的土壤质量含水量范围为10%～25%(相对含水量17.85%～89.3%)，最佳土壤相对含水量范围在65%～75%之间；不同分离物在相同土壤湿度培养下，多数分离物冬孢子的萌发率之间差异不显著。

PCR products of isolates that rpoB gene mutations were not found with PCR-SSCP were sequenced using double deoxidization chain terminating.

并对其中19株SSCP阴性的耐RFP株用双脱氧末端终止法进行测序分析。

PCR products of isolates that rpoB gene mutations were not found with PCR-SSCP were sequenced usˉing double deoxidization chain terminating.

并对其中19株SSCP阴性的耐RFP株用双脱氧末端终止法进行测序分析。

In order to understand the adaptation of the microorganisms under the extreme cold environment conditions, the physiological characteristics of the 5 bacterial isolates from the Muztag Am ice core under the different temperatures were investigated. The csp gene was also amplified by PCR technique and the specific primer targeted for the cold shock protein. The observation results showed that there were three phenotypes of microorganisms: psychrophiles growing from 0~23°С(the optimum temperature at 15°C), psychrotolerant with growth range at 0~40°C (the optimum temperature at 23°С), and the thermophile with growth range at 0~45°C(the optimum temperature at 30°C).

为了解冰川微生物的生理生长特征及其在极端寒冷环境条件下的冷适应特性，从慕士塔格冰芯分离菌株中选取了5个代表菌株，分别对这些菌株的生长温度和酸度范围进行了观测，并利用微生物冷诱导蛋白的保守氨基酸序列，设计了1对冷诱导基因csp的特异引物，并对其进行了PCR扩增。

Methods Mycobacterium tuberculosis strains, totally 158 isolates, isolated from Beijing, HeiLongJiang, Guangdong tinner Mongolia and Tibet, were subjected to IS6110-RFLP ,Spoligotyping and MIRU.

从北京市、黑龙江省、广东省、内蒙古自治区、西藏自治区五个地区收集158株结核分枝杆菌临床分离株，应用IS6110-RFLP、Spoligotyping及MIRU三种分型方法进行鉴定。

Preliminary study on molecular characterization of 11 isolates of Citrus tristeza virus.

柑桔衰退病毒分离株分子特征初步研究。

This study was conducted to investigate the CP/HinfⅠRFLP groups and molecular characterization of citrus tristeza virus isolates found in wild Chinese citrus plants.

了解中国野生柑橘上携带柑橘衰退病毒分离株的CP/HinfⅠRFLP组群构成和分子特征。

The isolates of S.sclerotiorum from different geographical regions and host-plants were cultured in the PDA culture medium. Mycelium from the culture was used for preparation of wheat seed inocula. The method for evaluation of resistance to soybean white mould in the field condition was established using silver paper trussing inoculum with minimally invasive on the soybean plants.

采用收集不同地区和寄主来源的菌核病分离物，经PDA培养基再生培养，再接种麦粒形成麦粒接种体，利用微创结合锡箔纸捆绑麦粒接种体的方法建立大豆菌核病田间接种鉴定方法。

Results The genetic variability among trypanosome isolates examined was obvious with an average genetic distance of 41.2%（ranged from 0 to 100%）.

方法利用移动遗传因子-PCR技术对26个锥虫虫株基因组进行扩增，同时利用邻位相连法对扩增产物进行聚类分析。

Methods Genomic DNAs of 26 trypanosome isolates were amplified by a mobile genetic elements-PCR technique and cluster analysis was performed based on the molecular profiles with Neighbor-Joining method.

目的分析锥虫亚属布氏锥虫、伊氏锥虫和马媾疫锥虫3个种以及布氏锥虫：指名亚种、冈比亚亚种、罗得西亚3个亚种间的遗传变异与进化关系。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。