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The morphological characteristic showed that the colonies were black green and the hyphae were septum, brown, Spore size was 11.07×25.18μm, mostly ellipse, with three septate , two central cells were larger than both terminal cells , brown to dark brown , terminal cells subhyaline to light Brown after cultured on PDA medium at 5d. The DNA sequence of ribosomal ITS of the isolates was found to match 99% with an ITS sequence of Curvularia verruculosus in GenBank.

为致病菌。该菌在PDA培养基上培养5d后,菌落呈墨绿色,菌丝褐色,有隔,分枝,分生孢子平均大小为11.07×25.18μm,褐色至暗褐色,椭圆形,具有3个隔膜,主隔膜明显加粗,中间两细胞较大。rDNA-ITS测序结果与GeneBank中的序列进行同源性比较发现菌株与Curvularia verruculosus的同源性高达99%。

A total of 29 isolates were selectively obtained from these rhizosphere soli samples based on their growth on nitrogen-free medium and their resistance to 100℃ for 10-15 min.

首先利用芽孢杆菌中芽孢的抗热性将土壤溶液在100℃沸水中煮10-15分钟,然后用选择性无氮培养基进行初筛得到29株菌落形态不同的菌株

Eighteen isolates of Phytophthora were isolated from diseased sponge gourd in Shenyang. Two typical Phytophthora strains that caused sponge gourd wilt were chosen in this experiment.

从沈阳地区丝瓜疫病的病组织及土壤中分离18株疫霉菌,从中选取2个代表菌株进行分类鉴定。

Results showed that all the three isolates grew well on the six solid culture materials while sporulation varied distinctly.

结果表明:3个菌株在6种固体培养基上均能正常生长,但其产孢量差异显著。

Variations in hyphae diameter, sporulation and vesicle numbers were noted between media and also between isolates.

菌株在菌丝体大小、孢囊形成数量、泡囊形成等方面存在着明显的差异,并随着培养基成分而变化。

A diode and capacitor circuit isolates the oscillator voltage from the main energy storage capacitor.

一个二极管和一个电容电路用来将振荡器电压和用来储存能量的大电容隔离开来。

The homologies of nucleotides and amino acids of G genes of the three isolates were 82.8%-83.1% and 88.0%-90.1% to those of aG, PV and ERA strains, and 86.7%-87.1% and 92.0% to those of CTN strain respectively. Conclusion Few variation was observed in the five rabies street virus strains isolated recently in China.

将3株街毒与人用疫苗株aG、PV和兽用疫苗株ERA的G基因进行比较,核苷酸同源性在82.8%~83.1%之间,氨基酸同源性在88.0%~90.1%之间;而与人用疫苗株CTN比较,核苷酸同源性在86.7%~87.1%之间,氨基酸同源性均为92.0%。

Results These 48 Staphylococcus epidermidis isolates had different resistance to aminoglycusides. The resistance and susceptibility to gentamicin was 69% and 31% respectively. The resistance, susceptibility and intermediary to netilmicin was 8%, 79% and 13%, streptomycin was 42%, 35% and 23% and amikacin was 15%, 75% and 10%.

结果 48株临床分离的表皮葡萄球菌对4种氨基糖苷类抗菌药物的药敏特征分别为:对庆大霉素的耐药率为69%,敏感率为31%;对奈替米星的耐药率为8%,敏感率为79%,中介率为13%;对链霉素的耐药率为42%,敏感率为35%,中介率为23%;对阿米卡星的耐药率为15%,敏感率为75%,中介率为10%。

The methods for determination of the complete genome sequences of potyviruses, carlaviruses and potexviruses were also established. The complete genome sequences of 13 viruses(20 isolates) were determined while 10 of them were the first report in the world.Five of them including Lily symptomless virus (NC_005138), Lily mottle virus(NC_005288),Onion yellow dwarf virus (NC_005029), Lily virus X (NC_ 007192) and Shallot yellow stripe virus(NC_007433) were selected as the standard sequences of those viruses by National centery of Biotechnology Information, USA.

建立了马铃薯Y病毒属、麝香石竹潜隐病毒属和马铃薯X病毒属成员的基因组全序列扩增技术,完成了13种不同植物病毒近20个分离物的基因组全序列测定,其中10个病毒的基因组全序列为国际首次报道,百合无症病毒(NC_005138)、百合斑驳病毒(NC_005288)、洋葱黄矮病毒(NC_005029)、百合X病毒(NC_ 007192)和葱黄条病毒(NC_007433)等5条序列被美国国家生物技术信息中心列为相关病毒标准序列的有。

This study was conducted to investigate the molecular variability of the coat protein gene of Citrus tatter leaf virus isolates collected from China.

了解中国CTLV分离物外壳蛋白基因的变异情况。

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