查询词典 isolates

Isolates from watermelon-growing areas were classified into 3 races like race 0, race 1 and race 2 which were 8 (17.4), 30 (65.2), and 8 (17.4%), respectively. Pathogenecity of race 0 isolates are weakest which only make Sugar Baby susceptible; isolates of race 1 are intermediately pathogenetic, they make Sugar Baby and Charleston susceptible Gray but Calhoun Gray resistant; isolates belonging to race 2 possess the strongest pathogenecity, which make 3 hosts susceptible. Isolates of race 0 mainly distribute over the central and southern Hebei province, whereas race 2 cover the middle and north of Hebei, isolates of race 1 exist in the whole watermelon-growing areas in Hebei province.

根据鉴别寄主对供试菌系的抗感反应，将46个西瓜枯萎病菌系划分为0号、1号和2号3个不同的生理小种，分别包括8，30和8个菌系，占供试菌系的17.4%、65.2%和17.4%；0号生理小种菌系致病性最弱，仅使品种Sugar Baby感病，以冀中和冀南居多；1号生理小种菌系致病力中等，使鉴别寄主Sugar Baby和Charleston Gray感病，而使Calhoun Gray抗病，分布在整个河北省西瓜种植区；2号生理小种的菌系致病力最强，使3个鉴别寄主均能感病，主要分布在冀中和冀北。

In 42 ACME-arcA-positive isolates, 8 isolates harbored SCCmec V, 8 isolates harbored class C1 mec complex and ccrAB_, 22 isolates harboring class C1 mec complex and 4 isolates harboring class C2 mec complex were negative for all known ccr allotypes.

本研究在ACME-arcA基因阳性菌株中发现整合酶基因ccr的一种新亚型ccrAB_(SHP.42株ACME-arcA基因阳性菌株，8株携带V型SCCmec，8株携带C1型mec基因复合体和ccrAB_，剩余26株携带C型mec基因复合体和未分型ccr。

Isolates CNR-1 and CNR-2 were from Brassica napus; CNA-1and CRW-1 from Rhododendron simsii and Oxalis corymbosa respectively. All isolates grew well in R-2 liquid medium and exhibited contractive movements. The colonies of all isolates were circinal and grain-like in solid medium. Through electron microscopy, all isolates exhibited helicity during their growth phase. All isolates could pass through a 0.22 μm filtrate membrane and resist to penicillin (2000 U/mL). They must grow in medium with serum.

这4株螺原体在R-2液体培养基中生长良好，都能通过孔径为0.22 mm的微孔滤膜；在R-2固体培养基上呈圆形或颗粒状菌落，菌落直径约50~600 mm；在生长的某个阶段可呈典型的螺旋状，菌体直径为37.04~370.40 nm，长度约0.89~11.88 mm；它们都能利用葡萄糖作为碳源，不能利用尿素；在不含胎牛血清的R-2培养基中，它们都不能生长；菌株CNR-1、CNA-1能强烈代谢精氨酸，而CNR-2和CRW-1不能代谢精氨酸；在氨苄青霉素钠浓度高达2000 U/mL的R-2培养基中，分离菌株生长良好。

The results showed:among the 7 isolates, five isolates of HY3、GY1－3、ZJ1－1、HP1、FC3 had same colony shape, irregular shape, liquidlike, slimy, opacity with smooth surface;the other two isolates had same shape, irregular shape, dry, opacity with coarse surface. By inoculating eucalyptus with the 7 isolates, the plants were infected apparently, and the young plants of eucalyptus in control experiment with tap water were not infected. By cultivating eucalyptus cuttings with the bacterial suspensions without EPS, the incidence of disease was very distinct,but compared with the former bacteria suspension,the incidence of disease has decreased at different degrees. By screening out two isolates of strong pathogenicity and two isolates of weak pathogenicity from the 7 isolates,making the bacterial suspensions with them to inoculate the young plants of eucalyptus, two treatments of cutlings and ramets with rats were set with 5 repetitions in every treatment, the results of data analysis showed: for the cutlings, the bacterial contents in upper and middle parts、upper and lower had significant difference;for ramets with roots, the bacterial contents in upper, middle parts, lower had significant difference between each other; For both the cutlings and ramets with roof, the bacterial contents in xylem and phloem had significant difference. The interaction between vertical and horizontal parts for the bacterial content had significant difference. For the two isolates of HY3 and 93B which were screened out at last,their activities of the cellulase were: 1.955ug/ and 1.288ug/ respectively, and had significant difference; the activities of pectase were: 1.325 ug/and 1.24ug/ respectively, and had no significant difference. The content of EPS extracted from the two isolates of HY3 and 93B was very different: 7.08x10-8ug/cell and 5.17x10-8ug/cell.

结果显示：7个菌株中，其中5个菌株HY3、GY1－3、ZJ1－1、HP1、FC3的菌落形态相同：不规则形状、流体、粘性、不透明、表面光滑；另外2个菌株93B、GN1菌落形态相同：不规则形状、干燥、不透明、表面粗糙；用7个菌株接种剪根桉树苗，发病情况非常明显，而自来水对照实验中桉树苗却不发病；无EPS菌悬液培养桉树剪根苗，发病率也很明显，但是相比原菌液，则发病率有不同程度的下降；从7个菌株中间筛选出来2个强致病性菌株和2个弱致病性菌株，用它们配制菌悬液培养桉树苗，设置剪根和不剪根两个处理，每个处理设置五个重复，数据分析结果显示：对于剪根苗，上部和中部、上部和下部的含菌量有显著的差异，中部和下部含菌量差异不显著；带根苗，上部、中部、下部含菌量彼此之间差异显著；不管是剪根苗还是带根苗，木质部和韧皮部含菌量之间的差异都非常显著；上中下与木韧交互作用中，含菌量差异非常显著；最后筛选出来的强弱2个菌株HY3和93B，它们的纤维素酶活性分别为：1.955ug/和1.288ug/，具有显著的差别；果胶酶的活性分别为：1.325 ug/和1.24ug/，没有显著的差别，而且HY3和93B两个菌株细胞分泌的胞外多糖的含量差异很显著，分别为：7.08×10-8ug/cell和5.17×10-8ug/cell。

Methods: We collected 121 of clinical isolates of Enterobacter, including 87 isolates Enterobacter cloacae, 34 isolates Enterobacter aerogenes, all of the isolates were detected by cefoxitin three-dimension test, drug resistance phenotypes estimation, cloxacillin disc synergy test and cloxacillin-potentiated disc diffusion test respectively to find high-level AmpC β-lactamase isolates, drug resistance phenotypes estimation were also used to detected high induction producing AmpC β-lactamases isolates, meanwhile, we took three-dimension test as standard method, and compared to other methods, then calculated the coincidence rates, sensitivities and specificities of them.

采用头孢西丁三维实验、纸片耐药表型推断法、邻氯西林纸片协同法、双纸片邻氯西林增效试验分别检测高产AmpCβ-内酰胺酶株，获得高产AmpC酶在肠杆菌属细菌中的表达状况。采用纸片耐药表型推断法检测诱导和高产AmpC酶，同时以头孢西丁三维实验为标准，其他三种方法与之相比较，分析它们检测高产AmpC酶的符合率、灵敏度和特异性。

According to the ability of the field isolates of Gibberella zeae to grow on the PSA with varying carbendazim concentrations, three sensitivity levels of isolates were determined in vitro. The sensitive isolates could grow at 0.5 μg/ml, but could not grow at 100 μg/ml. The high resistant isloates could grow faster than R at 50 μg/ml, and also could grow at 100 μg/ml.No low resistant isolates, that could grow fast at 1.4 μg/ml but could not grow at 50 μg/ml, were found among the field isolates.

根据在0.5、1.4、50、100 μg/ml等不同浓度的含药PSA平板上能否生长，将玉蜀黍赤霉田间菌株对多菌灵的敏感性划分为：敏感、中抗和高抗等3个水平，其中S菌株在0.5 μg/ml浓度下能生长，但在≥1.4 μg/ml浓度下生长受到完全抑制；R菌株在1.4 μg/ml浓度下能快速生长，在50 μg/ml浓度下能缓慢生长，但在≥100 μg/ml浓度下不能生长；HR在≥100 μg/ml浓度下仍能生长。

Three infectious bronchitis virus isolates named as Q1, J2 and T3 were isolated from proventricular tissue taken from vaccinated chicken flocks in China The biological characteristics of the three isolates were observed.

中文题名传染性支气管炎致腺胃病变毒株变异的分子基础及我国和东南亚分离株的分子流行病学研究副题名外文题名 Molecular basis for variation of proventriculus pathogenic IBV isolates and molecular epidemiology of IBV isolates from China and southeast Asia 论文作者蒋贻海导师陈溥言教授学科专业预防兽医学研究领域\研究方向动物分子病毒学及免疫学学位级别博士学位授予单位南京农业大学学位授予日期2002 论文页码总数127页关键词鸡传染性支气管炎传染性支气管炎病毒腺胃毒株馆藏号BSLW /2003 /S855 /9 从我国接种过传染性支气管炎疫苗的幼鸡群的腺胃组织中分离得到三个传染性支气管炎毒株Q1、J2和T3，并对它们的部分生物学特性进行研究。

In this study, isolate 23 microbes with lipase activity from Wu-Jie scrap heap at Ilan and Taichung Environmental Protection Section. When the microbes incubator in 50oC, there are isolates B61 and M63 that lipase activity are better than others. Brevibacillus borstelensis SH168 isolated from NTU resterant food waste compost is used as control check. Isolates B61, M63 and Brevibacillus borstelensis SH168 determine the lipase activity by the LB with tributyrin. The lipase activity of isolates B61 and M63 are 2~3 fold higher than Brevibacillus borstelensis SH168. So isolates B61 and M63 are more useful in producing lipase. Isolate B61 can grow at pH 5~9, and at temperature 20oC ~ 60oC; isolate M63 can grow at pH 6~10, and at temperature 30oC ~ 50oC. Isolates B61 and M63 were identified by 16S rRNA. Isolate B61 is Bacillus circulans, and isolate M63 is Bacillus species.

本研究，於宜兰五结垃圾场和台中市环保局厨余堆肥样品中共分离出 23 株具脂肪酶活性菌株， 23 株菌株中经过三油酸甘油酯的洋菜培养基於 50℃培养 5 天后，发现有 2 株菌的脂肪酶活性较强，分别为分离株 B61 和 M63；以本实验室先前自台大女九餐厅蔬果厨余堆肥中所筛选出的耐高温脂解菌 Brevibacillus borstelensis SH168作为本实验对照菌株，将分离株 B61、M63 及 Brevibacillus borstelensis SH168 3 株菌株於含有三酪酸甘油酯液态培养基培养并测定其脂肪酶活性，发现分离菌株之活性皆比 Brevibacillus borstelensis SH168 高出 2~3 倍，认为分离株 B61、M63 适於产生脂肪酶的菌株，分离株 B61 在 pH 5~9 皆可生长，生长温度介於 20 oC ~ 60oC；分离株 M63 在 pH 6~10 可生长，生长温度介於 30 oC ~ 50oC； 2 株分离菌株以 16S rRNA定序结果分离株 B61 为 Bacillus circulans，分离株 M63 为 Bacillus 属。

Of 549 isolates, 20 isolates (about 3.6% of the total isolates) showed significant activity against P. ultimum and 15 isolates (about 2.8% of the total isolates) against R.

结果表明，对终极腐霉、立枯丝核菌的半抑制稀释倍数（ID50）在10以上的活性菌株分别为20株（占总菌株的3.6%）和15株（占总菌株的2.8%）。

objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.

目的 用2组曲霉单克隆抗体建立特异性识别不同种类曲霉抗原的检测方法。方法采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心elisa法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。结果间接免疫荧光显示，用天然烟曲霉抗原免疫获得的单克隆抗体(mabs-1)可广谱识别多种曲霉分离株，而重组烟曲霉抗原获得的单克降抗体(mabs-2)仅能特异性结合临床和环境分离的烟曲霉抗原。用mabs-1建立的双抗体夹心elisa法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mabs-2)建立的双抗体夹心elisa法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心elisa法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。