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Rabbitfish were administered respectively to injections of DA (2μg/g wet weight) or PBS as controls. After 24 h, rabbitfish were killed and hepatic RNA was isolated.

我们用半定量PCR的方法，并用β-肌动蛋白作为外参照，在其指数期增长的范围内得到CYP1A，GSTA，GSTR，HSP70和ATP1A1 mRNA与β-肌动蛋白mRNA的比值。

RESULTS: The activity of heparanase in the mammalian cell lysate detected was much higher than that in the culture medium. The molecular mass of the heparanase protein expressed in the transfected COS7 and CHO cells was about 53×103 by western blot. CONCLUSION: The fulllength heparanase gene is isolated.

结果：分离扩增出1629 bp的人HPA全长cDNA序列并构建了其真核表达载体；在转染后的COS7细胞和筛选出的CHO细胞裂解液中检测到较高的HPA活性和相对分子质量大小约53×103的目的蛋白免疫印迹表达带。

Dendritic cells are the most potent antigen-presenting cells in human immune system. Our institute has been working on the region of DC development and DC-mediated immune therapy on cancers for a long time. A cDNA library of human dendritic cells has been set up and by large-scared random sequencing, we have isolated a lot of novel fulllength cDNA clones.

树突状细胞(Dendritic cells，DC)是人体内重要的抗原提呈细胞，本研究所对DC的分化发育及其介导的肿瘤免疫治疗的研究工作已进行多年，并在此基础上建立了人树突状细胞基因文库，通过大规模随机测序的方法开展了从DC中克隆新分子的工作，从2万余条EST中发现了许多新的分子。

The fulllength heparanase gene is isolated. The functional heparanase is expressed within COS7 and CHO cells and few heparanse is secreted into medium. The positive CHO cells expressing active heparanase is screened, which will help explore its function and screen its antibody and antagonists from a variety of sources.

本研究分离出人HPA编码基因全序列；在真核细胞内成功表达出有活性的人HPA，发现此酶在COS7和CHO细胞中表达在细胞内，很少分泌到细胞外；筛选出其恒定表达细胞株，为进一步研究HPA结构功能、开发其抗体及抑制剂提供了有力的工具。

The fulllength heparanase gene is isolated. The functional heparanase is expressed within COS7 and CHO cells and few heparanse is secreted into medium. The positive CHO cells expressing active heparanase is screened, which will help explore its function and screen its antibody and antagonists from a variety of sources.

本分离出人HPA编码基因全序列；在真核细胞内成功表达出有活性的人HPA，发现此酶在COS7和CHO细胞中表达在细胞内，很少分泌到细胞外；筛选出其恒定表达细胞株，为进一步研究HPA结构功能、开发其抗体及抑制剂提供了有力的工具。

Unless they succeed,the yield gains of the Green Revolution will be la rgely lost even if the genes in legumes that equip those plants to enter i nto a symbiosis with nitrogen fixers are identified and isolated,and even if the transfer of those gene complexes,once they are found, becomes poss ible.

除非他们能取得成功，不然的话，绿色革命的产量收益将在很大程度上损失殆尽，即使豆科植物中使这些植物有条件进入到与固氮细菌共生关系的基因可被辨识出来和分离开来的话，且即使这些基因综合体，一旦被发现之后，其移植得以成为可能的话。

Each of the strands in the web may be an independent point-to-point bus and isolated power line.

网络中的每股线都可以是独立的点到点的总线或者隔离的电力线。

Osteoblasts were isolated from the frontal parietal bone of ovariectomized goat and were confirmed by testing for multiple biological features.

选用摘除双侧卵巢的山羊颅盖骨，体外分离培养成骨细胞，并对分离的成骨细胞做多方面生物学特性鉴定。

Methods: Osteoblasts were isolated from parietal bone of the newborn SD rats (less than 24 hours) and cultured in minimum essential medium.

成骨细胞由新生24h SD大鼠头盖骨分离得到。

These mass lay-offs, it might be hypothesised, are more like natural catastrophes, such as famines, than isolated accidents that cause a few people to fall on hard times.

这些大规模的解雇时间，就有些像自然灾害比如饥荒带来的影响，比一些仅仅导致几人死亡的事件影响要大很多。

"Please accept this talisman as a token of our thanks."

请收下这个护身符以表达我们的感谢。

If I have that magic, I will be more acceptable in the society.

如果我拥有那种魔法，我将更加被这个社会所容纳。