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In order to identify the proteins which are responsible for the better fruit set in heat-tolerant variety than in heat-sensitive one, we used two dimensional electrophoresis systems to separate isolated proteins from developing anther and looked for the proteins up-/down-regulated in heat-tolerant variety. In doing so, two selected cultivars, CL5915, the heat-tolerant, and L4783, the heat-sensitive, were grown at the same time in a phytotron at both 30/25℃ and 25/20℃. The proteins isolated from developing anthers of heat-tolerant variety grown in 30/25℃, were subtracted to remove the common genetic background.

因此为了鉴定出在高温逆境下与番茄著果率提升有关之蛋白质体，我们利用亚蔬中心所提供耐热番茄(Solanum lycopersicum， formerly Lycopersicon esculentum)品系CL5915与不耐热品系L4783，藉由控制二品系间生长温度之差异（热逆境30/25℃与一般生长环境25/20℃），在雄蕊发育早期利用蛋白质体学技术分析可溶性蛋白质，比较并扣除共有之遗传背景之蛋白质后，将二品系间具有差异的可溶性蛋白质筛选出来，并进一步利用LC-ESI MS/MS进行蛋白质身份鉴定。

The toxicant was washed out after the isolated rat diaphragm was exposed to dDVP for 20 min,then the drugs were added; a:TCR=the height of tonic contraction of isolated rat diaphragm after DDVP and oximes/normal height

DDVP中毒20分钟后将毒剂洗掉10分钟，再加药对抗；a：TCR=毒物或药物作用后的TC高度/正常对照的TC高度；b：SCR=毒物或药物作用后的SC高度/正常对照的SC高度

Objective This paper is to study the mechanism of HI-6,PAM-Cl,PAM-I and obidoxime against the inhibition of DDVP on isolated rat phrenic diaphragm. Methods The neuromuscular transmission in isolated rat phrenic diaphragm was inhibited completely by 2.5×10-3 mol/L DDVP,thenwashed out the toxicants and the neuromuscular transmission was recoverd by incubation with the above oximes respectively.

用2.5×10-3 mol/L敌敌畏完全抑制大鼠离体膈神经膈肌传导，然后洗掉毒剂，加药对抗：用外加5.6×10-5 mol/L乙酰胆碱的方法来检验药物是以中毒酶的重活化作用为主，还是以直接生理对抗作用为主。

Until now, all taxol used in cancer chemotherapy and scientific research is isolated from yew tree or semi-synthesized from its precursors such as baccatin III, 10-deacetylbaccatin III and so on which all are isolated from this natural plant.

至今为止，所有用于临床治疗和科学研究的紫杉醇都是从红豆杉植物中直接分离或由巴卡丁III， 10-脱酰基巴卡丁III等化学半合成前体合成而得，而这些前体也是由植物中分离得到的。

The results showed: when TLC solvent system was: chloroform - methanol - diethyl amine = 90:9:1, alkaloids from lotus leaves were well isolated, and five components were isolated and identified by spraying Dragendorff reagent. The optimal conditions of HPLC solvent system were: methanol- water - diethyl amine = 75:25:0.0125. Under the conditions, components of alkaloids from lotus leaves were separated very well, and ideal RP-HPLC peaks were obtained.5、Methods of High Speed Counter Current Chromatography and preparative High Performance Liquid Chromatography applied to identify and purify alkaloids from lotus leaves were set up. When solvent system of HSCCC was: chloroform - methanol - water (pH=4.00)= 4:3:2, speed of chromatogram was 700rpm, flow speed of the mobile phase was 2mL/min, four pure components relatively were attained by isolation and purification.

结果表明：薄层层析溶剂系统为氯仿：甲醇：二乙胺=90：9：1，使荷叶中的生物碱达到了较好的分离效果，并用改良的碘化铋钾试剂喷雾显色，共分离鉴别出了5种荷叶生物碱；分析型HPLC分析检测的较优溶剂系统为甲醇：水：二乙胺=75：25：0.0125，使荷叶生物碱的各个组分达到了基线分离，并获得了较好的峰形。5、建立了高速逆流色谱和制备型HPLC技术分离纯化荷叶生物碱的具体方法。H(来源：ABC46论文网www.abclunwen.com)SCCC法在溶剂系统为氯仿：甲醇：pH4.00的水=4：3：2，色谱仪转速为700rpm，流动相流速为2mL／min的条件下，分离纯化得到了4个纯度较高的化合物。

The completed cDNAs were synthesized by using the SMART PCR cDNA technique, in which mRNAs isolated from the plants growing on MS liquid medium with 0mmol/L NaCl (sample 0) as driver, and the mRNA isolated from the plants induced with 200mmol/L NaCl for 24h (sample 2) as tester.

以北美海蓬子种子播种于0mmol/L NaCl MS液体培养基上幼苗（0号材料）的总RNA为Driver， 200mmol/L NaCl诱导24h幼苗（2号材料）的总RNA为Tester，运用SMART PCR cDNA合成技术分别合成全长cDNA。

A total of 83 endophytic fungi strains were isolated from fresh root samples of the plants growing on rocks or shrubs, and with red-purple lower leaf surface or with green lower leaf surface. The isolated strains were identified into 19 genera.

从附生于石上及生于杂木林或灌丛中、叶背绿色及叶背紫红色的五唇兰植株新鲜营养根段中共分离出83株内生真菌，鉴定为19个属，其中包括培养基筛选实验中分离出的30株14属。

The optimized conditions for isolation and culture of endophytic bacteria isolated from grasses in alpine area in this study. The different tissues of grasses were immersed in 0.1% of SDS for 15 min, 3% of NaClO for 3 min, 0.1% of mercuric chloride for 10 min, 75% of alcohol for 1 to 2 min. The endophytic bacteria were isolated after aseptic grinding and diluted to 10^(-3) and using TSA culture medium for 5 to 7 days under 28℃.

通过对高寒牧草内生细菌的分离培养方法研究，得出了优化的分离培养条件，即牧草不同组织器官用0.1%SDS浸泡15min、3%NaClO浸泡3min0.1%升汞浸泡10min、75%酒精1~2min处理后（各步间均用无菌水冲洗3~4次），研磨并稀释至10^(-3)，涂布于TSA培养基上，置28℃恒温条件下培养5-7d，可从高寒牧草组织中分离获得数量、种类较多的内生细菌。

Meanwhile, to solve the problem of large amount infeasible solutions caused by applying GA to distribution network planning, by use of graph theory and search technique a restoration scheme for infeasible solution is given, by means of restoring isolated nodes, isolated chain and closed loop, it is implemented to restore non-radial network to radial network.

同时为解决遗传算法应用于配电网规划时产生的大量不可行解的问题，借助图论知识和搜索技术给出了不可行解的修复方案，通过对孤岛、孤链和环进行修复，将非辐射状网络修复为辐射状网络。

Results Among 11 strains isolated from epidemic asthma-like pneumonia, 4 (36%) had extra six nucleotides inserted into the 3′end non-coding region of the F gene, whereas none of the F genes from 5 strains isolated from regular pneumonia had that insertion.

为了从分子基础来探讨RSV的致病性，本研究室对从不同地区、不同流行特征分离到的RSV进行了主要抗原结构蛋白的基因序列比较分析。

According to our present broadcasting situation,the thesis proposes using new technology to update and reform the present HFC. It also suggests broadcasting using self-advantage and arranging EPON in order to win the supermarket.

论文从我国广电的现状出发，结合实际，积极倡导采用新技术更新、改造我国现有的HFC网络；倡导广电现阶段利用自身优势，部署EPON网络来赢取市场。

A new general kinetic equation was derived in which the effect of the removal of condensation water was taken into account completely, and was applied to the polyesterification kinetic study of AA/HPHP and AA/NPG. It was found that the reaction order of self-catalyzed polyesterification was not constant. For these polyesterifications, the reaction order was 2.5 at a low temperature, while 3.0 at a high temperature.

考虑缩合水的排除对反应体系的影响，用体积浓度单位推导出一个新的聚酯反应总包动力学方程，并应用到AA/HPHP和AA/NPG两个聚酯化反应的动力学研究中，研究发现，自催化聚酯反应的反应级数并不是固定值，对于AA/HPHP和AA/NPG两个聚酯化反应而言，低温时反应级数为2.5，高温时反应级数为3.0。