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In this paper, adopted augmenting culture in nitrite bacteria culture media and the method of silica gel plate isolation,31 strains of bacteria were isolated from vegetable garden soil of our school. The color reaction test was carried out with Griess reagent and culture fluid, which was regarded as the index to determine producing NO2- or not. 13 strains which color were deep were obtained and they were further rescreened by doing nitrite test. A strain N4(coded N4,the same to the following)with higher rate of nitrosification was picked up after rescreened; A strain B08( coded B08 ,the same to the following) with higher rate of denitrification was obtained after isolated and rejuvenated from our lab conserving mixed denitrifying bacteria culture.

本研究采用亚硝化细菌富集培养基选择培养和硅胶平板分离法，从本校农场菜园土中分离到31株细菌，以格利斯试剂对培养液的反应颜色深浅作为指标衡量其产NO_2~-的多少，经初筛从分离株中筛选出13株格利斯试剂反应颜色较深的菌株，再对这13株菌作亚硝化试验，最终选出一株亚硝化速率较高的菌株(编号为N_4，下同)；另外，通过对本实验室保存的反硝化细菌混合菌液进行分离复壮，筛选出一株反硝化速率高的菌株编号为B_

Diversity and phylogeny of rhizobia isolated from root nodules of Sesbania cannabina in Jinshajiang arid river valley;2. To study the diversity and phylogeny of rhizobia from Sesbania cannabina in JinShaJiang arid river vally in Sichuan,48 rhizobia was isolated,nodulated,and studied by numerical taxonomy,BOXAIR-PCR,16S rDNA PCR-RFLP,sequences analysis of 16S rDNA and GSⅡ.

从金沙江干热河谷区田菁根瘤中分离得到48株根瘤菌，在对其进行回接实验后，采用数值分类、BOXAIR-PCR、16S rDNAPCR-RFLP、16S rDNA和GSⅡ序列分析方法对这些菌株进行研究，以揭示在金沙江干热河谷区这一特殊地理环境下田菁根瘤菌的多样性和系统发育地位。

Results: Among 327 gram-negative bacillus isolated from patients in ICU, the nonfermentative bacteria covered 65.41%(214/327), pseudomonas aeruginosa, stenotrophomonas maltophilia and ancinetobacter baumanni were the main nonfermentative bactria, the resistance rate of pseudomonas aeruginosa, stenotrophomonas maltophilia and ancinetobacter baumanni to imipenem were over 31%, and the isolated bacteria showed serious multidrug-resistance.

结果：共分离到的革兰阴性杆菌327株，其中非发酵菌214株，占65.41%(214/327)；非发酵菌以铜绿假单胞菌、嗜麦芽黄单胞菌、鲍曼溶血不动杆菌为主，对常用抗菌药物表现为严重耐药和多重耐药，对亚胺培南的耐药率达到31.0%以上。

From Whitmania pigra 10 compounds were isolated and structurally elucidated based on the spectral data, 9 of them were the firstly isolated from this species. They are choles-4-ene-3-one, uracil, succinic acid, hypoxanthine,xanthine, octadecanoic and 2 polyhydroxy-saturated fatty acid.

五对宽体金线蛭的化学成分进行了提取、分离、鉴定，从中分得10个化合物，其中9个为首次从该动物中分得，包括1个为甾体化合物胆甾-4-烯-3-酮、2个多羟基脂肪酸、琥珀酸、尿嘧啶、黄嘌呤、次黄嘌呤、脲苷、十八碳酸等。

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

In this study, an avirulent gene avrD (0.93 kb) isolated from a pathogenic bacteria Pseudomonas syringae PV. tomato and another avirulent gene Elicitin (0.294 kb) isolated from a pathomycete Phytophthora parasitica were ligated respectively with the non-specific pathogen induced promoters Pill and BG to construct plant expressive vectors pYH144 and pYHEt containing the two chimeric genes (Pill-avrD, BG-Elicitin).

tomato中获得的无毒基因avrD (0*93 kb)和从病原真菌Phytophthora parasitica中获得的无毒基因Elicitin(0.294 kb)分别与非专一性病原物诱导启动子Pill和BG组成含2个嵌合基因(Pill-avrD， BG-Elicitin)的植物表达载体pYH144和pYHEt。

In addition, Mycoplasma bovis, E. coli, Salmonella, Streptococcus, Staphylococcus and Aspergillus fumigatus was successfully isolated from the lungs and other tissues, however, no Pasteurella was isolated.

从肺等组织中分离到牛支原体以及大肠杆菌、沙门氏菌、链球菌、葡萄球菌和烟曲霉，没有分离到巴氏杆菌。

Among them, I was a new compound, II～IV and VI～IX compounds were isolated from pholidota for the first time, X and XIV compounds were isolated from P.chinensis for the first time.

其中化合物I是新化合物；化合物II～IV、VI～IX为首次从该属植物中分离到；化合物X和XIV为首次从该种植物中分离到。

Results In atropine group the function of isolated rats phrenic diaphragm was significantly less than that in control group (P＜0.01). In atropine plus PAM-CI group the function of isolated rats phrenic diaphragm was similar to control group.

结果 阿托品治疗组大鼠与空白对照组大鼠的单收缩和强直收缩有明显区别(P＜0.01)，而阿托品加氯磷定治疗组大鼠与空白对照组大鼠的单收缩和强直收缩无明显的区别(P＞0.05)。

Through a single shot experiment powered by a constant voltage charging device,it is found that when discharging in N2,the generator has the same output waveforms for both isolation configurations;but when discharging in SF6,it has a faster rise time for the resistively isolated configuration than that for the inductively isolated configuration,and the reason for this phenomenon was analyzed preliminarily.

通过恒压源充电的单次实验发现，发生器在氮气(N2)中工作时，两种隔离方式的输出脉冲波形基本一致，然而在六氟化硫(SF6)气体中工作时，电感隔离型相对于电阻隔离型的输出脉冲前沿较慢；对其原因做出了初步分析。

"Please accept this talisman as a token of our thanks."

请收下这个护身符以表达我们的感谢。

If I have that magic, I will be more acceptable in the society.

如果我拥有那种魔法，我将更加被这个社会所容纳。