查询词典 isolated substance

compound Ⅱ is isolated from Fagopyrum genus for the first time; compound 1 and 5 are isolated from Fagopyrum tataricum L.

化合物2为首次从荞麦属植物中分离得到，化合物1、5为首次从苦荞麦中分离得到。

So in our test aantagonistic germ was isolated from ginger soil and was studied. 108 strains of germs were isolated by tube dilution assay from the soil of gingerin Qingmu town,Bazhong city. Q6 strain was screened from these strains of germsthough the first screened and rescreened double with deck agar method andimproved aperture-strikeing method.

本实验从巴中市青木镇堆放腐烂姜并且又长出健康姜苗的根际土中分离出106株细菌，在涂布有姜瘟病原菌青枯劳尔氏菌的肉汤培养基上进行初筛，得到6株对青枯劳尔氏菌有较强拮抗作用的细菌，通过摇瓶发酵后利用发酵液，采用改良的琼脂孔扩散实验复筛，获得一株具有强拮抗作用的菌株，命名为Q6号。

Two strains of bacteria, named as TJ-1 and TJ-2, were isolated from enrichment with crude oil as a sole carbon source. Strain TJL-1 and Strain TJL-2 were isolated with hexadecane as a sole carbon source. The 16SrDNA identified results showed that the four strains of TJ-1, TJ-2, TJL-1 and TJL-2 were Bacillus pumilu , Acinetobacter venetianus , Oceanobacillus Picturae , Bacillus pumilus , respectively.

结果表明，利用2216E平板从以原油和正十六烷为碳源的富集液中各得到两株菌，分别为TJ-1、TJ-2和TJL-1和TJL-2，分子生物学方法鉴定结果表明，这4株菌分别为短小芽孢杆菌、威尼斯不动杆菌、 Oceanobacillus Picturae 和短小芽孢杆菌。

Furthermore, there is a great phenotypic diversity among the new strains isolated from the same host. Different groups consisted of the new strains from the same host, some were Rhizobium, some were Bradyrhizobium, such as the new strains isolated from leguminous plants of AMkora Prain, Trifolium repens Lam, Aeachynomene Mica L, Indigofera bungeana Steud.

同时，攀西地区豆科植物根瘤菌的多样性还表现在某些来自同一种寄主植物根瘤菌的多样性方面，如分离自山槐、白车轴草、合萌、铁扫帚上的同一种寄主植物内的根瘤菌分别聚在不同的表观群；且来自山槐、合萌、铁扫帚上的根瘤菌既有快生型，又有慢生型。

Several anti-tumor cell lines and carbonic anhydrase Ⅱ model were established. More than 10000 compounds and extracts from plants were screened using these models and about 10 compounds showed strong inhibitory activities. Two species of plants Inula helianthus-aquatica and Saussurea deltoidea were chosen to investigate chemical and bioactivity constituents based on the results of screening of extracts and fractions. 58 compounds were isolated and identified from these two plants, 6 compounds were found to be new and 14 compounds showed anti-tumor activities. We also studied the anti-tumor mechanism of the compound bigelovin isolated from Inula helianthus-aquatica. Bigelovin was found with apoptotic activity against cell line U266 by lowering the amount of important transcription factor E2F-1 which inhibits the cell cycle at G0/G1 stage. Docking study on 7 natural new phenolic inhibitors of CA Ⅱ and 3D-QSAR study on 61 sulfonamide inhibitors of CA Ⅱwere also investigated. The calculated results were in good correlation with experimental results.

基于以上两种重要疾病和研究组方向，本论文工作开展了多个肿瘤细胞株和碳酸酐酶Ⅱ靶标模型的建立和筛选工作，累计筛选1万多个/次样品，发现10余个活性较强化合物；从粗提取物筛选结果中选取两种抗肿瘤活性强的菊科植物水朝阳旋覆花(Inula helianthus-aquatica)和三角叶凤毛菊进行了系统化学和活性成分研究，分离鉴定了58个化合物，发现6个新化合物和14个抗肿瘤活性化合物；通过合作对水朝阳旋覆花中活性较强的bigelovin进行了抗肿瘤作用机制研究，发现它可能是通过降低G2期重要的转录因子E2F-1，致使细胞周期阻滞在G0/G1期，引起多发性骨髓瘤U266细胞凋亡；开展了7个新的天然酚类CAⅡ抑制剂的对接研究和61个合成的系列磺酰胺类CAⅡ抑制剂的3D-QSAR分析，结果表明计算与实验一致。

Compound 4 was isolated from the genus Lonicera for the first time and all 5 compounds were first isolated from the species of this plant.

经文献检索，5个化合物均为首次从该种植物中分离得到，其中化合物4系首次从该属植物中分得。

Compounds Ⅰ,Ⅱ were first isolated from Genus Lonicera, while compounds Ⅲ~Ⅺ were isolated from the plant for the first time.

化合物Ⅰ、Ⅱ为首次从忍冬属植物中分离得到，化合物Ⅲ~Ⅺ为首次从该种植物中分离得到。

A pair of primers producing 614bp amplification fragment were designed based on the sequence of the C\|polyhedrin gene of Bombyx mori CPV,and the expected amplification products were obatined when genomic dsRNAs isolated from purified BmCPV,DsCPV and Lymantria dispar CPV were used as templates.Genomic dsRNAs isolated from Heliothis armigera CPV and DNAs from Lymantria dispar nuclear polyhedrosis virus and DNAs from midgut tissue of healthy Dendrolimus spectabilis larva did not yield any amplification products.The detection limit of purified DsCPV genomic dsRNAs was 1.0pg.

依据家蚕质型多角体病毒质型多角体蛋白的核苷酸序列设计一对引物，从纯化的DsCPV\，BmCPV和舞毒蛾质型多角体病毒的基因组dsRNA可成功地扩增出长614bp的目的片段，从提取的健康松毛虫幼虫肠组织的DNA、舞毒蛾核型多角体病毒基因组核酸、以及棉铃虫质型多角体病毒基因组核酸，未能扩增出目的片段。

Six compounds have been isolated from Lysimachia congestiflora, and ardicrenin (5) is isolated from this species for the first time.

从聚花过路黄中分离得到6个化合物，其中化合物(5)为首次从该植物中发现。

The results showed that the optimized content of Mg~(2+) was 5mmol/L; dNTP was 200μmol/L; Primer was 0.5μmol/L; Taq polymerase was lU/50ul in the PCR system. Only one fragment of about 270bp was amplified within the genome of 12 wild species and 23 cultivars of Mallus Mill., which indicated that Ty1-copia-like retrotransposons were archaic components of apple genome. The nested insertion of near and within the reverse transcriptase gene of Ty1-copia-like retrotransposons wasn\'t found in apple genome.2 The reverse transcriptase conservative region of Ty1-copia-like retrotransposons were amplified from Gala apple using the optimized PCR system. The amplification product was isolated, cloned and sequenced. Forty-five clones containing reverse transcriptase conservative region were obtained (accession number: AY849580 -AY849592 and DQ105035-DQ105066). Cluster analysis of these sequences showed a great heterogeneity among RT domains isolated from the same genotype.

从苹果属12个野生种和苹果23个栽培品种中都扩增到了约270bp的目的产物，且所有试材的扩增结果一致，均无目的条带以外的产物扩增，说明Ty1-copia类逆转座子在苹果属植物中广泛存在，且存在历史可能较为久远；从扩增结果看不出该逆转座子的逆转录酶基因或附近区域在苹果基因组内有嵌套行为的发生。2、利用优化了的PCR方法从嘎拉苹果基因组克隆了45条Ty1-copia类逆转座子逆转录酶保守序列(登陆号分别为AY849580-AY849592和DQ105035-DQ105066)，结果表明，同一基因组来源的这些序列存在高度的异质性。

More direct, directer ; most direct, directest

径直的，笔直的

Do you know how to use a chain saw?

你知道如何使用链锯吗？