查询词典 isolated substance

Armillaria luteo-virens is a ectomycorrhizal fungus symbiotic with some species of Kobresia in Tibet Plateau, China. A strain was recently isolated from fruitbody tissue of the fungus. To determine whether the strain is the pure culture of the fungus, the internal transcribed spacer region and the first intergenic spacer region (IGS-1) of the nuclear ribosomal DNA of fruiting body and the isolated strain were sequenced and compared. Further, the 5.8S/ITS and IGS-1 sequences of the species were BLAST matched with GenBank database on homology and a phylogenetic tree was constructed based on 5.8S/ITS sequence and two phylogenetic trees were constructed separately based on two IGS-1 sequences.

首次从采自青藏高原、与高原牧草嵩草属Kobresia草本植物形成外生菌根的黄绿蜜环菌Armillaria luteo-virens子实体中分离获得一组织分离菌株，运用rDNA-ITS和rDNA-IGS-1测序技术对该组织分离菌株是否为黄绿蜜环菌的纯培养菌种进行分子鉴定，并基于黄绿蜜环菌的5.8S/ITS和IGS-1序列进行核酸序列数据库GenBank同源性检索比对、构建系统发育树。

Among them, strains of Bacillaceae occupied the predominance with 59.1% in various wheat organs. Oxytetracycline resistant strains of Bacillus licheniformis were isolated from wheat rhizosphere, root and stem indicating their high abilities of spreading. Opportunistic human pathogenic bacterial strains with oxytetracycline resistance such as Bacillus cereus and Stenotrophomonas sp. were also isolated from various wheat organs. No oxytetracycline resistant bacterium could be detected from wheat seeds.

土霉素抗性的Bacillus licheniformis在小麦根际土壤、根、茎等器官中都分离得到表明该抗性菌种在小麦内生系统中具有较高的传播性；具有土霉素抗性的人类机会致病菌Bacillus cereus和Stenotrophomonas属的细菌在小麦的根际土壤、根以及叶子中也均分离到，但是在小麦种子中没有分离到任何土霉素抗性的内生菌。

Results A total of 6 suspected colonies were isolated from 65 samples, all being Gram?negative small bacilli, and red for Gimanez staining. The PCR results confirmed that the six strains were all Bartonella , two isolated from Rattus losea and Rattus brunneusculue each, and one from Niniventer fulvescens and Suncus murinus each.

结果 从65 份样本中分离培养出疑似菌落6株，革兰染色镜检均见阴性小杆菌，Gimanez 染色为红色杆菌，经PCR 证实6 株均为巴尔通体，其中分离自黄毛鼠和屋顶鼠各2株，针毛鼠和臭鼩鼱各1株。

The major results obtained are: PCR-RFLP method is suitable for molecular identification of Shihu; 205 compounds were isolated from 10 species of Dendrobium, including 21 new compounds, and 28 compounds isolated from genus Dendrobium for the first time. Each of the extracts and compounds were screened for anticoagulant and antitumour activities separately. The results indicated that the ethyl acetate extract from Dendrobium chrysanthun has significant anticoagulant function; the antitumour function of the 4 bibenzyl components from Dendrobium chrysotoxum is stronger than that of phenanthrene, of which the erianin has significant anti-angiogenic effect both in vivo and in vitro; components with moderate or stronger antitumour activities were found in the extracts of D. thyrsiflorum, D. chrysanthun and D. trigonopus; quality standard for 5 species of Dendrobium was drawn up use active or indicative components as control.

重要研究结果有：经方法学研究，认为PCR-RFLP法适于作为石斛属药材分子标记鉴定方法；对10种石斛进行化学成分分离鉴定，共分得205个化合物，其中新化合物21个，首次从石斛属分得的28个；对提取物和各单体化合物分别作抗凝血、抗肿瘤等活性筛选，结果说明束花石斛乙酸乙酯部位有显著的抗凝血作用，鼓槌石斛的4个联苄类成分抗肿瘤作用强于菲类，联苄类毛兰素在体内外有显著抗血管生成活性，球花石斛、束花石斛及翅梗石斛均筛到中等或较强抗癌活性的成分；选择有活性或指标性成分作对照品，对5种石斛草拟了质量标准。

A total of 985 samples were isolated from laying hens ovary and caecal content samples. Salmonella were detected from 25.5% laying hens. None Salmonella were isolated from eggs in this study. The percentage of Salmonella positive specimens of ovaries was 1.4 times more than caecal content 95% C.

总计采取985只蛋鸡，鸡只的阳性率为25.6%(252/985)，蛋内容物则无沙门氏菌检出；沙门氏菌的样本总阳性检出串为18.2%(359/970)，卵巢的沙门氏菌检出率为蛋鸡肠内容物沙门氏菌检出率的1.4倍。

Avian influenza virus was isolated from the caecal tonsil, faeces, and kidney of the domestic ducks. The isolated virus was identified as a highly pathogenic H5N1, with a haemagglutinin proteolytic cleavage site deduced amino acid sequence of ... QREKRKKR/GLFGAIAG ... In order to determine the pathogenicity of the isolate, eight 6-week-old specific pathogen free chickens were inoculated intravenously with the virus, and all birds died within 24 h after inoculation.

病毒抗原可以从鸭的盲肠，扁桃体，肾脏及粪便分离出来，并且确定这种病毒是高致病性的H5N1，还从血凝素蛋白分解位点推断出病毒的氨基酸序列，为了了解独立群的发病机理，对8只6周龄鸡静脉注射这种病毒，结果所有的均在注射后24小时死亡。

Aian influenza irus was isolated from the caecal tonsil, faeces, and kidney of the domestic ducks. The isolated irus was identified as a highly pathogenic H5N1, with a haemagglutinin proteolytic cleaage site deduced amino acid sequence of ... QREKRKKR/GLFGAIAG ... In order to determine the pathogenicity of the isolate, eight 6-week-old specific pathogen free chickens were inoculated intraenously with the irus, and all birds died within 24 h after inoculation.

病毒抗原可以从鸭的盲肠，扁桃体，肾脏及粪便分离出来，并且确定这种病毒是高致病性的H5N1，还从血凝素蛋白分解位点推断出病毒的氨基酸序列，为了了解独立群的发病机理，对8只6周龄鸡静脉注射这种病毒，结果所有的均在注射后24小时死亡。

Roots of five species of Calanthe and two species of Phaius from north, midland and south of Taiwan were collected for the isolation of orchid mycorrhizal fungi. Total for 104 isolates of fungi were isolated. They are identified respectively by the traditional method or DNA sequence. Results showed thatTrichoderma and Mucor were most often isolated fungi. The DNA sequence results showed that only Xylaris was most common fungus.

由台湾北、中、南四处地方采集五种原生根节兰及二种原生鹤顶兰，进行此等原生兰花根部菌类分离，共分离出104株代码真菌菌种，分别再以菌落、菌丝和孢子等传统形态特徵鉴定菌种，结果发现以木霉菌及毛霉菌属内之菌株占优势，而利用核酸序列比对之鉴定结果则以炭角菌属内之菌株占多数。

Among them, 3 strains isolated from yellow water could convert lactic acid into ethanol and most strains isolated from Daqu could convert lactic acid into flavoring compositions such as ethanol, isobutyric acid, caproic acid or isovaleric acid etc.

其中，从黄水和大曲中分离到多种降乳菌，黄水中的3株菌可将乳酸转化为乙醇，大曲中的多数降乳菌可转化乳酸为异丁酸、己酸、异戊酸、乙醇等风味成分。

Results: Sixty-one constituents were firstly isolated and identified from the fruits of 3 species of Chaenomeles. Forty compounds in Chaenomeles lagenaria, thirty-four compounds in Xuan mugua (C.lagenaria from Xuanzhou) and 23 compounds in C.sinensis were isolated and identified respectively.

结果：从3种木瓜的乙醚提取部位中首次分离与鉴定出61个化合物，其中皱皮木瓜 40个化合物，宣木瓜(C.lagenaria 产自安徽宣州) 34个化合物，光皮木瓜 23个化合物。

More direct, directer ; most direct, directest

径直的，笔直的

Do you know how to use a chain saw?

你知道如何使用链锯吗？