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The results suggested that the hypovir-ulence - associated genetic elements existed in the cells of isolate Ep- 1PN unequally, andsome cells were shortage of the hypovirulence- associated genetic elements.1572 sexual progenies of isolate Ep- 1PN were obtained through single- ascospore -isolation from seven apothecia.

本文对核盘菌Ep-1PN菌株的弱毒特性及其相关特性的遗传特点进行了分析，并评估了Ep-1PN菌株及其弱毒因子防治菌核病的生防潜能，现将结果报道如下

Azole resistance was found in the candida albicans isolate from the renal transplant recipients, and there is no connection between incidence of resistance and isolate locus.

通过PCR扩增该基因上游启动子调控序列，转化质粒扩增后测序未找到耐药/敏感菌株在调控序列上的任何点突变。

Effects of peanut protein isolate concentration, glycerin concentration and gluten fortifier concentration on the peanut protein isolate-based edible film properties were studied.

以花生分离蛋白为原料，研究了花生分离蛋白质量分数、甘油加入量和增筋剂加入量对花生分离蛋白可食性膜膜性能的影响，通过正交实验确定制取花生分离蛋白可食性膜的最佳配方。

P28 紫色 Formation of a peptide bond 肽键形成–4侧链具有的反应–形成二硫键-其它反应 Tyr,His,Arg Review Isoelectric Point Titration滴定 of an amino acid 滴定 Gly幻灯片 18 His Lys Glu Reaction of amino acids Section 4 Protein isolation and purification 蛋白质分离及提纯 Working With Proteins Experimental techniques for protein analysis and characterization Purification steps A cell contains many types of proteins In the lab we want to isolate a single protein for experiments Purification steps We first grow cells or isolate tissues that contain the protein of interest We break open the cells to produce a crude extract Use centrifugation离心 to separate soluble from insoluble material We fractionate 分离 the protein mixture based on properties of such as size, charge affinity or solubility.

丹磺酰氯与氨基酸反应生成荧光性质强和稳定的磺胺衍生物，用于多肽链NH 用于多肽链 3末端氨基酸的标记烃基化反应(1) 2，4-二硝基氟苯2，4-dinitrofluorobenzene，二硝基氟苯(二硝基氟苯 DNFB也叫做试剂。DNFB在弱碱性溶液也叫做Sanger试剂试剂中与氨基酸发生取代反应，生成黄色化合物二硝基二硝基苯基氨基酸(dinitro phenyl amino acid， DNP氨基酸氨基酸)苯基氨基酸氨基酸(2)苯异硫氰酸酯(phenylisothiocyanate， PITC)在弱碱性条件下，与氨基酸反应在弱碱性条件下，生成苯乙内酰硫脲 PTH衍生物，(phenylthiohydantoin， PTH)衍生物，即PTH-氨基酸，此反应又称之Edman反应，该反应是蛋白质或多肽氨基酸序列测定常用的反应。

The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段，应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段；在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法；检测方法的特异性试验结果表明，该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应；检测方法的敏感性试验结果表明，通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg；对20份临床样品检测中，有10份为AIV阳性，其中有5份为H5亚型病毒阳性。

And PG activities of pathogenic isolate was obviously higher than that of nonpathogenic isolate, It is same that PG activities of all isolates were the highest in the seventh day after cultivating.

综上所述，PG活性的高低与大豆疫霉根腐病菌的致病力强弱有一定的关系，是该病菌的一种起致病作用的细胞壁降解酶。

To demonstrate experimentally the generation of velogenic NDV from a nonpathogenic waterfowl isolate, we passaged an avirulent goose isolate in chickens.

为了通过实验证明从水禽中分离的非致病型NDV可以转变为速发型病毒，我们通过在鸡体内传播鹅源性无毒力株，经气囊接种连续传代 9次，随后再在鸡脑内传代 5次。

Grisea have been obtained in recent years as well.In our previous work, an RIL population consisting of 304 lines was constructed from the indica cross Zhong 156/Gumei 2. Two genes conferring complete resistance to blast isolate 92-183 (ZC15) were primarily mapped by using 148 lines of the population. In the present study, genotype of the 304 RILs were analyzed and QTL mapping for partial resistance to 2 isolates of leaf blast and 1 isolate of neck blast were conducted.

本实验室曾经应用中156／谷梅2号构建了由304个重组自交系(recombinant inbred lines，RIL)组成的重组自交系群体，应用其中的148个株系对抗稻瘟病基因进行了初步的定位，本研究则进一步对整个群体的304个RILs的基因型进行了分析，对控制叶瘟和穗瘟部分抗性的QTL进行了定位。

While nine Korean isolates were similar to the Japanese isolate red sea bream iridovirus, one isolate was distinct from other iridovirus isolates.

而韩国真鲷虹彩病毒的10个株系和日本株系比较相似，其中一个病毒株系和其他株系明显有别。

Results: The homololgy of nuclcotide sequence between Wuhan isolates and Bejing isolate were semblable, had some difference between Japan isolate and much different with TT virus.

结果：武汉SEN病毒株与北京株同源性较高，与日本株有一定的差异，和TT病毒有较大差异。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。