查询词典 isoeugenol
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Condition optimization for its efficient fermentation was also carried out. The reaction and separation system with the addition of resin HD-8 and the isoeugenol/aquoeus biphasic system of the biotransformation of isoeugenol to vanillin by Bacillus fusiformis CGMCC1347 were set up. The isolation and purification of isoeugenol and vanillin in both systems were studied. The reuse of the immobilized cells in biphasic system was successfully applied.
SW-B9的基础上,对该菌株进行了生理生化特性鉴定以及16S rDNA序列分析,命名为纺锤芽孢杆菌CGMCC1347;继而对该菌株的发酵条件进行了研究,首次建立了纺锤芽孢杆菌CGMCC1347转化异丁香酚生成香草醛的树脂分离耦合体系和异丁香酚—水两相体系,同时对此二体系的底物和产物的分离提取进行了研究,成功实现了两相体系中固定化细胞的反复利用。
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This paper focused on the biotransformation of isoeugenol to vanillin by microorganisms. After the isolation of Bacillus sp. SW-B9 from soil, its physiological, biochemical characteristics and the 16S rDNA were analyzed.
本论文对微生物法转化异丁香酚生产香草醛进行了研究,在从土壤中筛选获得具有完全自主知识产权、能高效转化异丁香酚生产香草醛的菌株Bacillus sp。
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Strains beneficial to transform isoeugenol to vanillin were isolatied from soil based on the screening model using high concentration of isoeugenol.
在含有高浓度异丁香酚的筛选平板上获得的14支菌株均有转化异丁香酚生成香草醛的能力,其中SW-B9的转化能力最好,能从2%异丁香酚生成1.10 g/L香草醛,高于文献报道的菌种转化水平,并且其进一步降解产物香草醛的能力较为微弱。
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The biotransformations of isoeugenol to vanillin with various strains available in our lab were tested. It was found that several baterica including Bacillus subtilus 7658, Alcaligenes AS1.7617, Brevibacterium flavum 1.7a and Brevibacterium flavum AS1.013 could convert isoeugenol to vanillin.
对实验室所保藏的细菌、放线菌、真菌进行初步筛选发现细菌中枯草芽孢杆菌7658、粪产碱杆菌AS1.7617、黄色短杆菌1.7a、黄色短杆菌AS1.013能产生少量香草醛,最高浓度为0.31 g/L;放线菌中诺卡氏菌Nocardia sp。
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