查询词典 ionization

Furthermore, influences of different phosphate salts at various concentrations on the ionization efficiency of phosphopeptide were investigated systematically, finding that the signal intensity for phosphopeptide 48FQEEQQQTEDELQDK63 digested from β-casein were 5 to 8 times increased in an optimized condition with 10 mmol/L ammonium monobasic phosphate or 3 to 4 times increased with 10 mmol/L ammonium dibasic phosphate as additive to matrix 2,5-dihydroxybenzoic acid, respectively.

考察了两种适合于磷酸化肽离子化的基质类型2，5-二羟基苯甲酸和2，4，6-三羟基苯乙酮。用2，5-二羟基苯甲酸作为基质时，当加入10 mM 磷酸氢二铵时，磷酸化蛋白质β-casein的磷酸肽 48FQEEQQQTEDELQDK63的离子化效率可以增强5-8倍，当加入10 mM磷酸二氢胺时，磷酸肽的离子化效率可以增强3-4倍。

In the past twenty years the most processes of dissociation and ionization of NaK molecule were studied by using the weak laser fields.

在过去二十年中，人们采用弱激光场研究了很多NaK分子解离与电离动力学过程。

Paired ionization chambers used for the dosimetry of mixed gamma-neutron field in reactor, consist of a tissue-equivalent chamber which responds to both neutron and gamma radiation, and a nonhydrogenous chamber which is relatively insensitive to neutron.

采用对中子和γ辐射具有相似灵敏度的组织等效电离室与对中子不太灵敏而对γ辐射灵敏的无氢电离室配对，组成双电离室探测器，测量反应堆混合场中的中子和γ剂量率，具有较高的测量精度。

The total energies,ionization potentials and electron affinities of two geometric structures of O_4 are given.Compared with the calculated results of oxy...

与氧原子、普通氧分子O2和臭氧分子O3的计算结果比较，显示O4分子可以以正方形结构或正四面体结构形式存在，其中正方形结构更有可能是O4分子的真实空间结构。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Several aminated Fullerene derivatives were synthesized by the reaction of C〓 with phenylamine, diphenylamine and triethylamine etc., the phenomena of fragmentation and aggregation of the aminated Fullerene derivatives in UV laser desorption ionization mass spectrometer was observed, a few nitrogenous poly-Fullerene ions, such as C〓NH, C〓N〓H〓, C〓N〓H〓, C〓N〓H〓, C〓N〓H〓 etc.

对C〓富勒烯与苯胺、二苯胺、三乙胺等形成的加合物在紫外激光解吸电离作用下可以发生不同寻常的裂解和聚合现象进行了研究，认为其形成机理为：上述加合物在紫外激光解吸电离作用下，发生裂解产生C〓NH、C〓N及C〓等离子，然后它们之间发生聚合，形成如：C〓NH、C〓N〓H〓、C〓N〓H〓、C〓N〓H〓、C〓N〓H〓等含氮原子的聚富勒烯离子。

Three kinds of molecular weight determination methods, i.e., size exclusition chromatography, sodium dodecyl sulphate-polyacrylamide gel electro phoresis and high performance liquid chromatography / electro spraying ionization-mass spectrometry (HPLC/ESI-MS) are introduced to study of the attribute of collagen hydrolysates.

应用体积排阻色谱、聚丙烯酰胺凝胶电泳、高效液相与电喷雾质谱联用(HPLC/ESI-MS)这三种蛋白质分子量分析测试技术，来表征胶原水解产物的分子量，以寻求较好的胶原水解产物分子量的测试方法

Then a method for measuring n_e through detectingI_ in ionization region is presented.Use micro-arc discharge to prepare TiO_2 photocatalyzer.

与n_e之间的数值关系，提出一种通过测量电晕放电电离区内I_的大小粗略测定n_e的方法。

Delayed Extraction Matrix Assisted Laser Desorption/Ionization Mass Spectrometrywas used to high resolution analysis of mix based DNA samples,using the binary matrices 3 HPA(3 Hydroxy 2 Picolinic Acid)/PAand substrate nitrocellose film.

本文使用延迟引出基体辅助激光解吸/电离质谱( DE- MALDI- MS)法，以3-羟吡啶甲酸和吡啶甲酸混合液为二元基体，硝化纤维膜为基底，对混合碱基组成的 DNA样品进行高分辨率测定，获得满意的结果：DNA2 0 - mer分子离子峰分辩率为 8769，DNA 36- mer为 1 650 ，DNA 54- mer为 843，前二者的组成分子离子峰各同位素峰清晰可辩。

This thesis take the phthalic acid as an example to intraduce the nonlinear data processing method in the mensuration of polybasic weak acid ionization constant, point out the defect of the linear data processing method, and give the main steps of nonlinear fitting.

以邻苯二甲酸为例，介绍多元弱酸电离常数测定实验数据的非线性处理方法，指出这类实验数据线性化处理的问题，并给出非线性拟合的基本步骤

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。