查询词典 investigate thoroughly

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Another world, either abstinent thoroughly, or liberated thoroughly, will come.

它将带来另外一个世界，一个要么彻底禁欲、要么彻底解放的世界。

Efficacy: This product is a transparent facial soap with olive essential oil and honey as main materials. It has double efficacies of Cleansing and Moisturizing, which c ould hydrate skin as well as thoroughly cleanse dirt and aged keratose. It is easy to produce fine and rich foams, which can quickly enwrap and insulate dirt, leaving the face thoroughly cleaned. After using it, your skin will feel relax and moist, and fall in love with everyday cleansing!

功能：含本品采用橄榄精华油和蜂蜜为主要原料的透明洁面皂；具备能够彻底清除肌肤污垢及老化角质的"洗净力"和"滋润肌肤"的双重功效；迅速起泡，柔软细腻的泡沫能迅速包裹、分离污垢，彻底洗净脸部；没有紧绷感，并能留住滋润，让洗脸也变成一种享受。

Efficacy: This product is a transparent facial soap with olive essential oil and honey as main materials. It has double efficacies of Cleansing and Moisturizing, which could hydrate skin as well as thoroughly cleanse dirt and aged keratose. It is easy to produce fine and rich foams, which can quickly enwrap and insulate dirt, leaving the face thoroughly cleaned. After using it, your skin will feel relax and moist, and fall in love with everyday cleansing!

功能：含本品采用橄榄精华油和蜂蜜为主要原料的透明洁面皂；具备能够彻底清除肌肤污垢及老化角质的"洗净力"和"滋润肌肤"的双重功效；迅速起泡，柔软细腻的泡沫能迅速包裹、分离污垢，彻底洗净脸部；没有紧绷感，并能留住滋润，让洗脸也变成一种享受。

R Efficacy: This product is a transparent facial soap with olive essential oil and honey as main materials. It has double efficacies of Cleansing and Moisturizing, which could hydrate skin as well as thoroughly cleanse dirt and aged keratose. It is easy to produce fine and rich foams, which can quickly enwrap and insulate dirt, leaving the face thoroughly cleaned. After using it, your skin will feel relax and moist, and fall in love with everyday cleansing!

r功能：含本品采用橄榄精华油和蜂蜜为主要原料的透明洁面皂；具备能够彻底清除肌肤污垢及老化角质的"洗净力"和"滋润肌肤"的双重功效；迅速起泡，柔软细腻的泡沫能迅速包裹、分离污垢，彻底洗净脸部；没有紧绷感，并能留住滋润，让洗脸也变成一种享受。

Therefore, the purpose of this topic research is to set out from the sociological angle, carry on a creatively thorough research on the formation and development of this block in ancient times,specifically including its characteristics and the reasons for their formation, and the relationship between it and Fujian culture especially Fuzhou cultrue;and to firstly answer the question why there were so many famous people living in this block, thoroughly analyze the ultimate motivity that push its development in ancient times even its great achievement in modern times;so as to fill in the blank of this academic research,and to make people's know about this block more thoroughly and completely,and then realize that the great achievement that it had achieved in morden times was not a historical fortuity,but deeply relied on the base of historical culture.

因此，本课题研究的目的在于从社会学的角度出发，对古代三坊七巷的形成和发展进行深入的研究，具体包括它的特点、特点的形成原因，与福建文化特别是与福州文化的关系进行了首创性的研究，初步回答了古代三坊七巷名人辈出的原因，深入分析了推动古代三坊七巷不断发展直至近代取得辉煌成就的深层动力因素，从而弥补了国内此项学术研究的空白，使人们对古代三坊七巷的了解更加系统、深入，认识到它在近代的辉煌不是历史的偶然，而是有着深厚的历史文化底蕴作依托。

Therefore, the purpose of this topic research is to set out from the sociological angle, carry on a creatively thorough research on the formation and development of this block in ancient times,specifically including its characteristics and the reasons for their formation, and the relationship between it and Fujian culture especially Fuzhou cultrue;and to firstly answer the question why there were so many famous people living in this block, thoroughly analyze the ultimate motivity that push its development in ancient times even its great achievement in modern times;so as to fill in the blank of this academic research,and to make peoples know about this block more thoroughly and completely,and then realize that the great achievement that it had achieved in morden times was not a historical fortuity,but deeply relied on the base of historical culture.

因此，本课题研究的目的在于从社会学的角度出发，对古代三坊七巷的形成和发展进行深入的研究，具体包括它的特点、特点的形成原因，与福建文化特别是与福州文化的关系进行了首创性的研究，初步回答了古代三坊七巷名人辈出的原因，深入分析了推动古代三坊七巷不断发展直至近代取得辉煌成就的深层动力因素，从而弥补了国内此项学术研究的空白，使人们对古代三坊七巷的了解更加系统、深入，认识到它在近代的辉煌不是历史的偶然，而是有着深厚的历史文化底蕴作依托。

A structure of space-time diversity virtual double-route weighting interference mitigating is proposed, and the corresponding practical algorithm is derived in detail using block matrix inversion formula and matrix inversion theory for mitigating interference signals from both other cells and other users of local cell in the DS-CDMA up-link communication case. Performances of such algorithm are thoroughly analyzed through simulation and analytical results. The up-link and down-link channel characters and their relationship of DS-CDMA communication system are thoroughly analyzed, and the relationship of space-time diversity weighting matrix between time division duplexing and frequency division duplexing these two different transmission ways is derived.

本论文深入地比较了DS-CDMA通信系统的上传情形与下传情形的信道特性及其它们相互之间的关系；深入地分析并得到了时分双工和频分双工两种不同的传输模式下的上传情形与下传情形的空时分集权系数矩阵之间的数量关系，从而使得能够利用在基站端所拥有的上传情形的空时分集接收权系数矩阵信息以及其他信息在基站端直接形成空时分集发射权系数矩阵；推导了下传情形移动用户接收机端的最优组合接收策略及其算法；进一步地，对时分双工传输模式下的下传情形的通信系统性能和频分双工传输模式下的下传情形的通信系统性能进行了深入的比较仿真研究。

This mistake ruin circle Brilliant Garden thoroughly, Chinese circle all of the landscape of the Brilliant Garden ruin, strange spend different stones to all dig to betray money, fill lake to build a farmland, sell brick, several 10-year times, the park of ten thousand parks thoroughly ruins in the Chinese people's hand, but the debt record on the foreigner head.

这一误就把圆明园彻底毁掉了，中国人将圆明园的山水全部毁掉，奇花异石都挖出卖钱，填湖造田，盗卖砖瓦，几十年工夫，万园之园就彻底毁在中国人民之手，帐却记在洋人头上。

Later, under the untypical parasternal four-chamber view which can thoroughly display the ostium of coronary sinus, the catheter was promoted further to the ostium of coronary venous sinus. Then the echocardiography technician adjusted the transducer of TTE in order to thoroughly confirm the position of the catheter tip. After confirming the position of catheter tip in the coronary venous sinus, the operator inserted the catheter into the coronary venous sinus for 3～4 mm farther. It was noted that if resistance was encountered by operator, the operation must be stopped, which was the same as x-ray fluroscopy as image guiding. TTE guiding His bundle elactric cahteter、high right atrium electric cahteter and right ventricular electric catheter in site: It is difficult using TTE singly since there are too many crossroads in inferior venous.

本研究结果显示，(1)单独应用TTE作为影像学引导进行冠状窦电极导管置放的成功率为93.8％～96.7％，并且应用TTE作为影像学引导放置专用冠状窦电极导管和普通电极导管的成功率和放置时间在统计学上无显著差异；(2)TTE作为影像学引导，除过度肥胖患者外，可完全替代X线引导进行冠状窦电极导管置放，包括鞘管和扩张管的放置、指引导丝在静脉而非动脉内的证实均可由TTE完成引导，且较X线C型臂有一定优越性；(3)TTE结合普通X线胃肠透视机作为影像学导引，可顺利完成心内电生理检查时导管的安全到位，而不需昂贵的X线C型臂，可将心内电生理检查扩大到广大的基层医院；(4)TTE较X线更易和更早期发现介入治疗的并发症如急性心包填塞等，对并发症的防治甚至挽救病人的生命至为重要；(5)动物实验研究表明，TEE可引导射频导管消融术时大头电极导管成功到位。

Hanna: That's over now, isn't it?

都结束了，对吗

You must be ill. You look so pale.

你一定是病了，你的脸色苍白。