查询词典 investigate in secret

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

As the colastantdevelopment of market economy and our country join WTO,only depend on this simple clause of the newly—increased crime of infringing upon business secret in criminal law of 1997 insufficient in an all-round way attacking criming effectively,and clause this have a great deal of perfect spaces,this thesis explains,analyses from such respects as the concept of the business secret and the composition important documents and asserting and punishing of the characteristic,crime of infringing upon business secret,etc.,pointing out the deficiency that the crime of infringing upon business secret exists and putting forward thc criminal legal system idea of perfecting the crime of infringing upon business secret.

随着市场经济的不断发展以及我国加入WTO，仅靠1997年《刑法》中增设的侵犯商业秘密罪这一简单条款，不足以全面、有效打击犯罪，并且该条款存在诸多不完善之处。从商业秘密的概念和特征、侵犯商业秘密罪的构成要件和认定及其处罚等方面进行阐述、分析，指出了侵犯商业秘密罪的刑事法制存在的不足，并提出了完善侵犯商业秘密罪的刑事法制构想。

How when we are in a Doubt or Hesitation, whether to go this Way, or that Way, a secret Hint shall direct us this Way, when we intended to go that Way; nay, when Sense, our own Inclination, and perhaps Business has call'd to go the other Way, yet a strange Impression upon the Mind, from we know not what Springs, and by we know not what Power, shall over-rule us to go this Way; and it shall afterwards appear, that had we gone that Way which we should have gone, and even to our Imagination ought to have gone, we should have been ruin'd and lost: Upon these, and many like Reflections, I afterwards made it a certain Rule with me, That whenever I found those secret Hints, or pressings of my Mind, to doing, or not doing any Thing that presented; or to going this Way, or that Way, I never fail'd to obey the secret Dictate; though I knew no other Reason for it, than that such a Pressure, or such a Hint hung upon my Mind: I could give many Examples of the Success of this Conduct in the Course of my Life; but more especially in the latter Part of my inhabiting this unhappy Island; besides many Occasions which it is very likely I might have taken Notice of, if I had seen with the same Eyes then, that I saw with now: But 'tis never too late to be wise; and I cannot but advise all considering Men, whose Lives are attended with such extraordinary Incidents as mine, or even though not so extraordinary, not to slight such secret Intimations of Providence, let them come from what invisible Intelligence they will, that' I shall not discuss, and perhaps cannot account for; but certainly they are a Proof of the Converse of Spirits, and the secret Communication between those embody'd, and those unembody'd; and such a Proof as can never be withstood: Of which I shall have Occasion to give some very remarkable Instances, in the Remainder of my solitary Residence in this dismal Place.

有时，我们会陷入无所适从的境地，踌躇不定不知道该走哪条路才好。这时候，内心常常会出现一种暗示，指示我们走这条路，虽然我们原来想走的是那条路。不仅如此，有时我们的感觉、愿望、或我们的任务明明要我们走那条路，可是心里忽然灵机一动，要我们走这条路；这种灵机也不知道是从哪里来的，也不知道出自什么影响，可就是压倒了原来的一切感觉和愿望，使我们走这条路。结果，后来的事实证明，如果我们当初走了我们自己想走的路，或者走了我们心目中认为应该走的路，我们则早已陷于万劫不复的境地。反复思索之后，我自己定下了一条规矩：每当自己心里出现这种神秘的暗示或冲动，指示我应做什么或不应做什么，我就坚决服从这种神秘的指示，尽管我不知道为什么该这么做或该这么走，我知道的只是心里的这种暗示或冲动。在我一生中，可以找出许许多多这样的例子，由于我遵循了这种暗示或冲动而获得了成功，尤其是我流落到这个倒霉的荒岛上以后的生活，更证明了这一点。此外还有许多例子。当时我若能用现在的眼光去看待，是一定会意识到的。但是，世上有许多道理，只要有一天能大彻大悟，就不算太晚。我奉劝那些三思而后行的人，如果在他们的生活里，也像我一样充满了种种出乎寻常的变故，或者即使没有什么出乎寻常的变故，都千万不要忽视这种上天的启示，不管这种启示是什么看不见的神明发出的。关于这一点，我不准备在这里讨论，也无法加以阐明。但这种启示至少可以证明，精神与精神之间是可以交往的，有形的事物和无形的事物之间是有神秘的沟通的。而且，这种证明是永远无法推翻的。关于这一点，我将用我后半生的孤寂生活中一些很重要的例子加以证明。

Article 25 Secret-involving information systems may be classified into secret, confidential and top secret in an ascending sequence according to the secret degree of the information to be processed by them.

第二十五条涉密信息系统按照所处理信息的最高密级，由低到高分为秘密、机密、绝密三个等级。

Billy Graham reportedly [16] told a group of ministers in Greensboro [17], North Carolina, when asked, What is the secret to your ministry:"If there is a secret to my ministry, it is not my secret, it is the secret of the Holy Spirit. There are two things that I rely on heavily—first is the convicting power of the Holy Spirit, and second, I say 'the Bible says.'"

格雷厄姆被问到他在格林斯堡市的团体服事有什麼奥秘时，他说〞如果我的服事有奥秘，那不是我的奥秘，那是圣灵的奥秘，有二件事是我深深倚靠的─第一是圣灵的能力，第二是圣经所说的。

Never allow your government to operate in secret, with secret prisons, secret wiretapping laws and secret war "evidence" that is never made public.

不要让您的政府的运作秘密，秘密监狱，秘密窃听的法律和秘密战争"证据"，这是从来没有公诸于众。

Firstly, there are less secure and efficient threshold schemes; secondly, the research on multi-secret sharing is rather weak; thirdly, usual verifiable secret sharing don't consider the partners' anonymity, while it is just people's expectation. Last but not least, the problem of variety on shares is becoming a more obvious restrict of efficiency, especially in the facets of the lost and being districted of shares. On the application of verifiable secret sharing, there are also two inadequates : on the one hand , verifiable secret sharing has been used in much threshold cryptography but in less verifiable signatures.

然而，经过认真总结、分析该领域目前研究的发展状况（具体结果将在第一章给出），我们发现在可验证秘密分享的研究方面，仍有下列四个不足之处：一是现有的安全、高效的门限方案不多；二是对多秘密分享的研究非常薄弱，在已有的文献中还见不到安全、实用的可验证多秘密分享方案；三是通常的秘密分享方案没有考虑秘密分享者的匿名性，而这恰恰是人们所希望的；四是秘密分享者的动态管理问题已经越来越明显的制约现有方案的安全，尤其是密钥份额丢失或被破坏时如何恢复更是没有被深入探讨。

The handtailor nourishes the tonic: Four team's members necessary, it is said can let the human who was already utterly exhausted become the vigor abundant medicine Sword eight: The generation seals gives the strongest god of death the title Alone blinkers eyeshade: The technical development bureau manufacture may unlimited absorb the spirit strength unceasingly the monster, the sword eight uses it to constrain own strength and to store up its spirit strength Shakes the spot: The anaesthetic, the spirit presses the low person so long as stains can remain unconscious, but does not have the function to the spirit strength excel in person Puts on/to collapse the spot: Strong effect anesthetic, even if the spirit strength excel in person also with difficulty resists The day treads gorgeously: So long as pours into the spirit strength to be able in the sky soaring, is the corpse soul unique precious stage prop Energetic body: The secret mobile most important special spirit has one above, thus can cut the soul knife the main body to force the extension to absorb obtains the embodiment Handcuff: May seal up criminal's spirit strength The secret is mobile: The explanation did not have temporarily, found said again Garrulous bee: The bee ninth generation teaches other people, in six brother younger sister's sixth human of bee tree top damask silk, after joins the punishment armed forces the given name which inherits from her great grandmother there Punishment armed forces: The secret mobile first division, is completely in five division's highest organizations.

特制滋养强壮剂：四番队的成员必备，据说能让已经疲惫不堪的人变得活力充沛的药剑八：世代封予最强死神的称号独眼罩：技术开发局制造的可以无限制不断吸收灵力的怪物，剑八用它来压抑自己的力量并将其灵力储存起来震点：麻醉药，灵压较低的人只要沾上一点就会昏迷不醒，不过对灵力高强的人没有作用穿点/崩点：强效麻醉剂，即使灵力高强之人也难以抗拒天踏绚：只要注入灵力就能在天空飞翔，是尸魂界独一无二的珍贵道具精神体：隐秘机动最重要的特殊灵具之一，能将斩魄刀的本体强制转摄其上从而得到具像化手铐：可以封住犯人的灵力隐秘机动：解释暂无，找到了再说碎蜂：蜂家第九代传人，六兄妹中的第六人蜂梢绫，加入刑军后从她曾祖母那里继承来的名号刑军：隐秘机动第一分队，是全部五分队中的最高机构。

The main content of this thesis includes six parts. First of all, it is a comprehensive systematic introduction about RSA algorithm including the present application situation and principle of RSA algorithm----producing big prime numbers and secret keys, the encryption arithmetic for information and the decryption for secret information, which establish the theory foundation for achieving concrete; secondly, it introduces some basic conception of RSA digital signature and theory of digital signature realizing process; thirdly, it introduces the basic principle of MD5 algorithm; fourthly, it states design and realization of RSA digital signature in detail. The main modules includes producing RSA secret keys, implementation of RSA encryption algorithm and decryption algorithm, producing message digest and realizing digital signature and verification by RSA; the fifth, it carries on testing entirely, analyzing and improving for this system;The sixth, it analyses the security of RSA digital signature and points out the development direction of RSA digital signature.

本文主要研究的内容包括：第一，对RSA算法进行了全面系统的介绍，包括RSA算法的应用现状和原理—大素数的产生、密钥对的产生、对明文的加密运算和密文的解密运算，为具体实现打下了理论基础；第二，介绍了RSA数字签名的一些基本概念和数字签名的理论实现过程；第三，对MD5算法基本原理的介绍；第四，详述了RSA数字签名的设计与实现，主要实现的模块包括RSA密钥的产生，RSA加密算法和解密算法的实现，消息摘要MD的生成以及利用RSA算法实现数字签名和签名的验证；第五，对该系统进行了整体的测试和分析改进；第六，分析了RSA数字签名的安全性，指出了RSA数字签名的发展方向。

Here's a list of Lex Luthor's known lairs straight from the Superman Encyclopedia: a complex of buildings held aloft by a giant dirigible high above the stratosphere, a glass-enclosed city of ancient, weird design, an abandoned factory, a gigantic man-made meteor floating in outer space, an abandoned barn, a secret underground lab, a giant spaceship, a secret mountaintop laboratory, an electronics firm, a hidden laboratory on the outskirts of Metropolis, a laboratory hideout that has been lined with lead to conceal it from Superman's X-ray vision, a massive fortress-like citadel on a lonely mountaintop north of Metropolis, a lonely farmhouse in the mountains north of Metropolis, a secret lead-lined subterranean hideout built into the side of a grassy hill, and the elaborately equipped Luthor's Lair, Luthor's Lair II, and Luthor's Lair No.

关于莱克斯·卢瑟已知的据点，超人的百科全书上是这样记载的："他的藏身处包括由巨大飞船托浮在半空的复杂建筑群；古代样式的玻璃城，设计诡异；废弃的工厂；悬浮在外太空的巨大人造陨石；废弃的谷仓；秘密的地下实验室；巨大的太空船；建于山顶的秘密实验室；电子公司；藏匿于城郊的实验室，这个实验室通体灌铅，能够躲过超人的X光眼；巨大的堡垒--它就像孤零零伫立在城市北面山顶上的一个据点；坐落在城市北面山上的孤零零的农舍；还有建在绿岭山脊上的秘密铅制地下室；以及装备精良的卢瑟堡一号，二号和5号。"

These rules reflect some inherent feature of self and nonself, and they are the basic detection criterion of GECISM.

这些规则反映了"自我"和"非我"的内在特征，是GECISM进行"非我"检测的判据。

Until today, the media from China and the outside world still uses the term "Post 89". Regardless to whether it is for commercial gimmick or convenience, everybody still remember "Post 89", a term which carries a strong Cantonese accent.

时至今日，国内外艺术媒体还是在沿用&后八九&这个称谓，不管当初是为了商业噱头还是容易上口好记，反正大家现在是记住了&后八九&这个广东口音很重的词。