查询词典 investigate and ascertain

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

AbstractObjectives1 Correlate bone mineral densityto vertebral compressive strengths.2 Consummate the technique for percutaneous polymethylmethacrylate vertebroplasty and observe the pattern of PMMA imaging distribution.3 Determine the strength and stiffness of osteoporotic with or without vertebroplasty with PMMA bone cement.4 Correlation of BMD,insertion torque and pull-out strengths of pedicle screws.5 Ascertain whether augmentation with PMMA bone cement can enhance pedicle screw fixation in the osteoporotic spine.6 Ascertain whether augmentation pedicle screw fixation with PMMA bone cement can enhance the stability of unstable thoracolurner burst fractures of osteoporotic spine.

目 的1、测试椎体压缩强度，分析强度与骨矿物质密度（bone mineral density，BMD）的相关关系。2、观察经皮椎体成形术后聚甲基丙烯酸甲脂（polymethylme- thacrylate，PMMA）骨水泥在椎体内的形态学分布及影像学表现。3、比较PMMA骨水泥骨质疏松椎体成形术前/后椎体最大抗压力和压缩刚度的变化。4、分析椎体BMD、螺钉最大旅入力矩和最大拔出力三者之间的关系。5、分析PMMA骨水泥强化骨质疏松椎弓根螺钉后的螺钉的最大轴向拔出力和拔出刚度的变化。6、评价PMMA骨水泥强化骨质疏松椎弓根螺钉脊柱内固定对不稳定型胸腰椎损伤的即刻稳定性和反复载荷后的稳定性。

Moreover, the author analyzes some special questions which including the ascertain to scope in multinational-corporations; the question of scope changed in the middle of a year, such as the ascertain to date and the number of beginning, select the combination method to period statement and the disposal to the question of inside trade's unconsummated profit.

并针对合并范围的一些特殊问题进行了探析，如：在多层控股关系下合并会计报表合并范围的确定；合并范围在年度内发生变动的各类问题如并表日与年初数的确认、时期报表的合并方法选择以及内部交易未实现损益等问题的处理。

Then,we improve CSFP Model in two aspects:when we ascertain the frequency of default,we use negative binomial distribution to substitute Poisson distribution so that the model can measure the data better;when we ascertain the loss distribution of default,we use Γ-distribution to substitute normal distribution.

其创新与特色一是对贷款组合的风险暴露数进行分段，在每一频段上分别确定风险暴露数分布，进而可以更准确的描述各笔贷款的风险暴露数。二是在确定风险暴露数分布时，用Γ分布替代了传统的正态分布，解决了贷款违约的风险暴露数分布的厚尾问题，进而解决了大宗贷款违约的风险度量问题。三是确定违约频率时，用负二项分布来代替传统的泊松分布，解决了违约频率的方差通常要大于其均值的分布拟合问题。

Objective:To ascertain the frequency of prothrombin gene G20210A variant and to investigate whether this polymorphism is related to arterial thrombosis in Chinese people.

目的 ：研究凝血酶原基因G2 0 2 10A(FⅡG2 0 2 10A)多态性在中国人群中的分布频率及其与急性心肌梗死和缺血性脑卒中之间的相关性。

ABSTRACT: Objective To investigate the effects of felodipine on sclerotin metabolism of SD rats through intragastric administration and ascertain its effect on osteoporosis in order to afford animal experiment evidence for clinic.

目的 明确非洛地平灌胃对SD大鼠骨质代谢的作用，确定其对骨质疏松的影响，为临床用药提供直接的动物实验证据。

Chapter 9 is conclusion and future prospect. There are five innovative findings in the paper. Firstly, the evolvement of extensions and connotations of two reengineering styles is analyzed based on their high risk, and finding their trends of amalgamation. With this, the concept of corporation reengineering is then redefined combining core competence theory and ideas of process change management, and decompounding it into two parts: pre-reengineering and post-reengineering. Its inner relationships are analyzed and explained by economics theory. Secondly, establishing a analysis framework of reengineering risks, risk factors of foreign BPR are analyzed and concluded systematically for the first time. Reengineering risks are divided into three parts: plan, design and executive based on process and project management, then risk factors system is abstracted. Thirdly, 72 reengineering corporations in Jiangsu province are investigated on risk condition. Furthermore, risk factors in every part are analyzed and reduced experimentally by factor analysis method, and a risk factors system to suit Chinese corporation's reengineering is established. Fourthly, setting up a choice model of reengineering projects, the objective optimization model is presented to ascertain the weigh of each factor, a method of sequencing reengineering projects is presented so that risk of each reengineering project can be evaluated and compared effectively. Fifthly, an integration relationship model among IT, BPR and strategy is established aiming at productivity paradox derivative from IT and its dual effects on BPR, IT investment priorities in BPR is narrated deeply.

本论文的创新点体现在五个方面：1）基于上述两种重组方式的高风险性，对它们的外延和内涵演变进行了分析，发现它们日益融合的趋势；然后结合核心能力理论和流程变革管理的思想对企业重组的概念进行了界定，将其分为&前重组&和&后重组&两部分，并对其内部关系进行了详细分析，同时应用经济学理论对其进行了解释。2）建立了重组风险的分析框架，并首次较为全面地分析和归纳了国外企业重组的风险因素，根据重组的过程和步骤以及项目管理的观点，将重组风险划分为计划、设计和执行3个部分，并在此基础上提炼出企业重组的风险指标体系。3）运用现场访谈、问卷调查的方法，调查了江苏省72家企业重组的风险情况，然后利用因子分析法对每一部分的每一风险因素进行实证分析，对风险指标进行了约减，建立了比较完善的适合我国企业的重组风险指标体系。4）建立了重组变革方案的选择模型，然后针对不同的重组方案提出了确定指标权重的目标优化模型，并基于三角模糊数之间两两比较的可能度概念，提出了一种重组方案排序法，从而可以有效地对各种变革方案的风险进行评估和比较。5）针对信息技术所造成的&生产率悖论&现象及其对重组的双重影响，建立了信息技术、企业重组和企业战略之间的整合关系模型，并对重组中信息技术投资的优先权问题进行了深入的阐述。

Long spring railroad branch office at to was investigate, was investigate the index sign, factor of the section target mission results and peripheral results and comprehensive as a result carry on investigate, all press the way that the grade evaluation conversion investigates the score to carry on the processing.

长春铁路分局在对被考核者、被考核部门目标任务绩效与周边绩效的指标、因素及综合结果进行考核时，均按等级评价转换考核分数的方式进行处理。

It shall include a review of Contract requirements; a check to assure that all materials and/or equipment have been tested, submitted, and approved; a check to assure that provisions have been made to provide required control testing; and plan mock ups when appropriate; examination of work area to ascertain that all preliminary work has been completed; and a physical examination of materials and equipment to assure that they conform to approved shop drawings or submittal data and that all materials and/or equipment are on hand.

这应包括对合同要求的审核；确保所有材料和/或设备都已经过试验、提交和批准的检查；确保已提供了要求的控制试验的检查；适当时，对模型进行规划；对工作区域进行检查，以确认已完成了所有预备工作；对材料和设备一的次物理检验，以确保符合经批准的施工图纸或提交数据且已获得所有材料和/或设备。

The main research results in this dissertation can be given as following:Firstly, the bidding strategies and affections of generation companies on the TOU power price are analyzed; Supply function model is employed to simulate the bidding strategies of generation companies in power pool. Some meaningful results are obtained through the proposed equilibrium equations model, when different bidding parameters are selected to maximize profit of suppliers, such as the the numeral of generation company, the block bidding, and power demand elasticity. Based on these results, the affections between the bidding strategies and the TOU power price are discussed.Secondly, the important principles consider the factor of bidding strategies of generation companies and consumers gaming strategies are proposed to constitute the new TOU power price model under present electricity market. Based on these pricinples a new mathematical model of TOU power price is constructed, to evade electricity market risk, partition the peak-valley, ascertain the consumers' response curve, and protect the ambilateral profits.Thirdly, the affections of the TOU power price strategies for reducing the network loss, adjusting node voltage, improving load curve of power system, and protecting the consumers' benefits in electricity market are analyzed with applications of a city real time load data of Jiangsu province.

针对"厂网分开，竞价上网"的电力市场运营模式，本文主要完成了以下研究工作：1研究了发电商不同的竞价上网策略，利用供给函数均衡方法，建立了发电商的竞价上网策略模型，给出了市场均衡解的具体解法；讨论了不同条件下发电商的竞价策略对市场的影响，并获得了发电商的最优上网竞价策略，明确了竞价上网与峰谷分时电价之间的影响因素；利用电力系统负荷曲线，建立了发电商最优竞价策略与峰谷分时电价之间的相互联系，通过仿真算例分析了峰谷分时电价与发电商最优报价之间的相互影响。2提出了"厂网分开，竞价上网"电力市场模式下，考虑发电侧竞价和用户侧博弈等风险因素影响，峰谷分时电价理论建模在规避电网企业运营风险，保护供电方与用户双方的利益、确定用户响应曲线、划分峰谷时段、设置合理的电价拉开比等方面所应遵循的基本原则，在此基础上建立了适合电力市场模式的峰谷分时电价模型。3从原理上分析了需求侧实行峰谷分时电价策略，对削峰填谷，提高负荷率，改善负荷曲线形状，降低电力系统的电能损耗和电压损耗等方面的影响，并进行了仿真验证。

Diabetes is a social disease that affects several million people worldwide.

糖尿病是一个社会性疾病，全世界有数百万人罹患此病。

I'll call you on Friday to see if we can reschedule our luncheon meeting at your convenience.

我星期五会给您打电话在您方便的时候我们重新安排我们的午餐约会。