查询词典 inverted

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Whether it be that their antipodal situation has affected their brains, or whether it is the mind of the observer himself that has hitherto been wrong in undertaking to rectify the inverted pictures presented by his retina, the result, at all events, is undeniable.

不论是当地人们和他在地球的位置正相对，影响了他们的大脑，或者他这个观察者本身一直以来都从来没有正确地翻转自己视网膜所成的像，结果都一样不可否认。

Observations on intact transparent ovaries under inverted differential interference microscope showed that the antipodal cells in the embryo sac disappeared at 4 days after pollination.

用粳稻84_15与水稻喜峰杂交，在杂种第二代选育出中新一号比秋光增产11%，已种植F10代农艺性状保持一致。

Objective Some severe cases of the inverted nipple cannot be corrected by simple method, especially if the nipple never comes up from the areolar level by manipulation.

一些严重的乳头内陷病例仅靠简单的方法是不能纠正的，特别是一些乳头内陷不能用手挤出乳晕皮肤外的病例是不可以达到正常水平的。

The seismic facies study indicates that the developed structures include prograding configuration, rhythm-inverted distributary mouth bar on braided delta front and turbidite in bathyal lake mud, belonging to typical braided delta system.

根据沉积背景和沉积环境标志的研究，发现阿南地区存在多个煤层、发育直立的植物根茎化石和泥裂构造，地震相中前积结构发育，前缘相中反韵律的分流河口砂坝发育，在半深湖泥岩中浊积岩发育，属典型的辫状河三角洲沉积体系。

In depth wavefield migration, in order to extend the 2D implicit method to 3D case, a blocklike diagonal matrix has to be inverted.

在深度偏移方法中，把二维隐式方法推广到三维，就会面对一个分块对角矩阵求逆问题。

In order to study how the single rDNA copy takes the role of the double rDNA copies and the function of the inverted repeat, we cloned the 16s rRNA and its leader sequences from Brassic napus and Vicia faba chloroplasts, by using E.

由于rDNA位于反向重复顺序内，所以有反向重复顺序类型的每一个DNA分子上有两个rDNA拷贝，而无反向重复顺序类型的只有一个rDNA拷贝。

Growth characteristcis of primary and passage cells obtained from human fetal calvaria were observed with an inverted microscope.

用碱性磷酸酶染色、钙结节茜素红染色等方法对所获得的成骨细胞进行鉴定。

Methods Human PDLC cultured in vitro was collected and seeded on Col, HA, HA/Col scaffolds crosslinked by carbodiimide. The influences of scaffolds on cell adhesion and growth were observed by MTT assay. The growth of human PDLC on scaffolds was observed through inverted phase contrast microscope and scanning electron microscope.

将体外培养的人牙周膜细胞接种到碳化二亚胺交联的胶原、透明质酸及透明质酸/胶原支架上；MTT法检测支架对人牙周膜细胞黏附、生长的影响；并用倒置相差显微镜和扫描电镜观察形态变化。

The red fish son and the Carthamus tinctorious inverted image have formed agglomerates of Hong Yun , we have been reluctant to part will do in pool.

水池里，红色的鱼儿和红花的倒影形成了一团团的红云，使得我们依依不舍。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。