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All sequences were aligned with S genes in GeneBank by BLAST software.The result indicated that all 10 fragments contained domain encoding 5 high conservation regions(C1,C2,C3,RC4,C5) and hypervariable regionof rosaceous S-RNase.RHV and intron characterized S gene furthermore.

测序结果表明，这些DNA片段的核苷酸序列中都具有编码蔷薇科S-RNAase 5个高度保守区域：C1、C2、C3、RC4、C5的序列、编码高变区的序列以及变异度很大的内含子序列，并且其高变区和内含子序列具有扁桃S基因特异性。

To develop an iphRNA vector for seed-specffic expression targeting the fad2 gene in Brassica napus L., an inverted repeated unit of the 537bp segment of the fad2 gene was first cloned into a medium vector, the pllurrican Vector, with a spliceable fad2 intron sequence in between.

通过PCR扩增分离到甘蓝型油菜种子特异性表达的NaPin启动子序列(11147bp)以及油酸脱饱和酶基因(fad2)的一个537bp的片段，并将它们分别克隆到pGEM-T easy载体中。

Sequence analysis and homology alignment showed that MD1 had 93% similarity with matK in maize chloroplast genome, a gene encoding maturase included in type Ⅱ intron splicing of RNA transcript; MD2 had 99% similarity with gene PP2C, encoding serine / threonine protein phosphatase type 2C in extremely drought tolerant Sporobolus stapfianus; and MD3 had 99% similarity with rice gene encoding metacaspase, belonging to aspartic acid specific cysteine caspases.

序列分析和同源性比对表明，MD1与玉米叶绿体基因组中编码参与RNA转录本Ⅱ型内含子剪切的成熟酶的matK基因有93%的相似性，MD2与极端耐旱的Sporobolus stapfianus的丝氨酸／苏氨酸2C型蛋白磷酸酶基因PP2C的相似性达99%，MD3与属天冬氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase基因有99%的相似性。

Results of sequence and homology analysis show that MD 1 has 97% similarity with mark in maize chloroplast genome,a gene encoding RNA maturase involved in group Ⅱ intron splicing of RNA transcript;MD2 has 99% similarity with the gene serine/threonine phosphorylase 2C in Sporobolus stapfianus;and MD3 has 99% similarity with rice the gene encoding metacaspase,an arginine/lysine-specific cysteine protease.

序列分析和同源性比对表明，MD1与编码成熟酶的玉米叶绿体基因matK有97%的相似性，MD2与极端耐旱植物Sporobolus stapfianus编码丝氨酸/苏氨酸蛋白磷酸酶的PP2C基因有99%的相似性，MD3与属精氨酸/赖氨酸特异性半胱氨酸蛋白酶类的水稻metacaspase酶基因有99%的相似性。根据MD2片段序列，结合电子克隆和RT-PCR方法，克隆出一条1731 bp的全长cDNA序列，它编码388个氨基酸。

By the method of polymerase chain reaction-single strand conformation polymorphism, this paper studied the genetic polymorphism of exon 1 and intron 1 in the aquaporin gene of seven species of Stipa in the meadow steppe, typical steppe, and desert steppe of Inner Mongolia.

以分布于内蒙古的草甸草原、典型草原、荒漠草原上的7种针茅属植物为材料，利用聚合酶链反应－单链构象多态性方法，分析了水孔蛋白基因的外显子1和内含子1的遗传多态性。

A highly conserved sequence of 210 bp of AtNHX1 gene that encodes a Na+/H+ antiport protein was isolated from A.thaliana,the 210 bp fragment was amplified as RNAi target site and used in construction of RNAi vector,and was inserted into pHANNIBAL as reverted repeat with an intron.The expressional box was linked into the vector of pART27,NPTⅡgene is the screening marker in this vector.

利用RNAi研究植物基因功能，可选择靶基因的一段单一序列使基因家族中的单个成员沉默；也可以选择某一基因家族的多个成员具有的一段同源性很高的保守序列，设计针对这一序列的dsRNA表达载体使多个基因同时沉默，RNAi成为功能基因组学研究的重要手段。

Methods 605 cases with stroke and 313 healthy controls were recruited in this study, and the stroke group was delaminated into two subgroups according to the history of hypertension. SNP rs1800780, an A to G base change located in intron 12 of the gene, was used as a genetic marker.

以rs1800780位点为遗传标记，采用聚合酶链反应和限制性片断长度多态性，检测605例缺血性卒中患者和313例对照组人群NOS3基因的多态性。

In the study, RT-PCR and sequencing were employed to detect the expression levels of CCK-B receptor, CCKBRi4sv and gastrin mRNAs in 30 human gastric carcinoma and their corresponding normal tissues, 10 gastritis and 2 autopsied normal stomach specimens as well as in a gastric carcinoma cell line SGC-7901 cells.

本研究首先用RT-PCR及测序方法检测了30例人类胃癌组织及癌周正常胃粘膜、10例胃炎、2例尸检胃组织及胃癌细胞株SGC-7901中CCK-B受体、CCK-B受体的异位剪接变异体(intron 4-contained variant of cholecystokinin B receptor，CCKBRi4sv)及胃泌素基因的表达。

Sequence analysis results showed that this gene is consisted of 2453 base pairs including one 1538 bp intron and two exons of 212 bp and 703 bp respectively.

序列分析结果表明该基因为 2 45 3bp ，含有一 15 38bp的内含子和两个分别为 2 12bp和 70 3bp的外显子。

We obtained robust estimates of the contribution of parallel gain to the observed sharing of intron positions between eukaryotic species separated by different evolutionary distances .

我们将真核物种根据不同的进化距离进行分类，并获得了这些真核物种之间平行获得对内含子位点的一致性的贡献的强有力的估计。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。