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By separation and combination analyses of nuclear rDNA ITS and the cpDNA (matK gene, rpl16 intron and atpB-rbcL spacer) sequences, the interspecific relationships were discussed, and the classification review and biogeographic analysis were done for all species bearing raceme or spike of one to many umbels with prophylla at base.

运用nrDNA ITS及cpDNA(matK、rpl16、atpB-rbcL)序列的单独和联合分析探讨了这些类群的种间关系，并对世界菝葜属中具有一至多个伞形花序排列成圆锥花序或穗状花序、花序梗上有关节的所有种进行了分类学研究及生物地理学研究。

In this experiment Bamei cooperation pigs, three kinds of different Duroc pigs ear of the Organization for materials, the use of PCR-SSCp technology, pig LPL gene intron 4 and part of exon 5 to carry out a number of Analysis of state.

本实验以八眉猪、合作猪、杜洛克猪3种不同猪种的耳缘组织为材料，利用PCR-SSCp技术，对猪LPL基因第4内含子及部分第5外显子进行了多态性分析。

A novel mutation in the donor splice site of intron 1 of FECH gene is first reported in a Chinese family with EPP; this mutation may lead to a deficiency of FECH gene and serve as a molecular basis of development of erythropoietic protoporphyria.

报道一FECH基因第1内含子供体剪接位点的新突变，该突变可能引发FECH基因缺陷，是EPP家系中患者发病的分子基础。

Mechanism of intron splicing in filamentous fungi might be similar to that in other eukaryotes.

二、丝状真菌内含子的剪接可能与其他高等真核生物相似，但它是否影响基因表达呢？

VWF gene is one of the most polymorphism genes. There is a lot of allele in 40th intron of vWF gene, and the heterozygous rate is higher.

vWF基因是人类最具多态性的基因之一。vWF基因40号内含子中的VNTR存在多种等位基因，有较高的杂合频率。

Methods The 26th exon, the 30th exon and the 21st intron of gene GP Ⅱb in 110 individuals were amplified by polymorphism and sequenced to investigate whether there was linkage among the polymorphisms of the gene. Human platelet antigen-3 (HPA-3) gene frequency was detected by Fok 1 enzyme in 147 patients with hematologic diseases, and was compared with that in 110 normal individuals. Forty-four patients who received apheresis platelet transfusion repeatedly were randomly divided into the HPA-3 homotype group and the control group. The antibodies of the platelet were detected after 3 times of platelet transfusion.

聚合酶链反应－单链构型多态性分析检测110例正常人GP Ⅱb基因第26和30外显子(Exon 26， Exon 30)及21内含子(Intron21)基因多态性，进行基因序分析，研究这些基因多态性是否存在连锁关系；应用Fok1酶切法对147例血液病人人类血小板抗原－3(human platelet antigen-3， HPA-3)基因进行分型，并与110例正常人进行比较；将接受单采血小板输注的44例血液病人随机分为HPA-3同型输注组和对照组，血小板输注3次以后检则血小板抗体。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

In order to make Maca be introduced to China, the basicproblems related to introduction and planting such as morphology classification, regionlayout, tissue culture and allelopathy were carried out. And the results were as follows:(1) The numerical Taxonomy of Maca and fifteen plants of Lepidium in China wasinvestigated by cluster analysis and the results showed that the method of numericaltaxonomy could be applied for Lepidium and its results were basically consistent with thatof morphology taxonomy and molecular marker such as Pi intron, ITS and cpDNA.

本文围绕玛咖在中国引种栽培的基础性和关键性问题，进行了形态学分类、区域规划、组织快繁以及克生现象等的研究，取得以下成果：(1)采用聚类分析，对 15 种中国独行菜属植物以及玛咖进行数量学分类，结论与经典形态学分类以及用 Pi 内含子序列、ITS 序列和 cpDNA 序列进行分类的结果基本吻合，玛咖与家独行菜、碱独行菜的亲缘关系最近，相似系数分别为 0.839、0.722。

In this study, we sequenced the intron 2 of mitochondrial nad1 gene to study the phylogeny of Oryzeae, in particular emphasizing the systematic position of Porteresia, a monotypic genus of Oryzeae with the only species P. coarctata.

本研究选用nad1基因的第2内含子序列探讨了稻族Oryzeae的系统发育关系，并着重分析了单型属Porteresia的系统学位置，并在此基础上探讨了nad1基因内含子序列的系统学意义以及空位性状在系统发育重建中的价值。

We used direct PCR to isolate the rps7Ⅵintron region hosting the f copy of U17 snoRNA coding sequence from 49 fishes, which include 42 ostariophysan species plus 7 outgroup species.

我们测定了49种鱼类、67条rpS7 intron6序列，得到U17 snoRNA f copy，共计55个单倍型，包括42种骨鳔鱼类，7种外类群。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。