查询词典 intron

Common buckwheat ( F. esculentum ) is an allogamy crop, which is heterogony. There is heterogeneity in the species. To reveal the genetic diversity within cultivar population, In order to explore the sampling strategy that can reveal genetic diversity of buckwheat resources on the molecular level. PCR with intron-splice junction primers and long random primers was employed to reveal the genetic diversity within nine common buckwheat varieties and one tartary buckwheat cultivar population among different individuals. We established the sampling strategy in its genetic diversity study. Then we used the established sampling strategy, and the same PCR markers technique,to analysis the genetic diversity of 86 common buckwheat resources, the main results are as follows

甜荞是花柱异长自交不亲和的异花授粉作物，品种内存在一定的异质性，为了准确揭示甜荞遗传资源的遗传多样性，本研究采用因子分析法对86份甜荞遗传资源农艺性状数据进行分析；采用长随机引物和内含子切接点引物的PCR分子标记技术，对9个甜荞品种和1个苦荞品种不同个体间的遗传多样性进行了分析，建立了甜荞资源遗传多样性研究的取样策略；并利用建立的取样策略，采用PCR标记技术，对这86份甜荞资源的遗传多样性进行了分析，取得如下主要结果

And there were great variations in the length of intron in the region of EC8-EC11 in population of cotton bollworm.

在棉铃虫中编码EC8～EC11的DNA序列内含子变异很大，具较高的多态性。

In addition, in vertebrates the sequence, 5' CURAY 3', which is called the branch point sequence, is present in the intron 10-40 bases upstream of the 3' signal sequence.

此外，在脊椎动物的序列， 5 ' curay 3 '性（ r = purina ），即所谓的分支点序列，是目前在内含10-40基地上游的3 '信号序列。

The bond is broken releasing the 5' end of the intron and attaching it to the branch point sequence, The intron now forms a tailed loop structure called a lariat.

债券是破碎释放的5 '端的内含，并注重向分支点序列，内含子，现在形成了一个尾式闭环结构称为套索。

The splicing reaction involves two transesterification steps which ligate the exons together and release the intron as a branched lariat structure containing a 2'5' bond with a conserved A residue in the branchpoint sequence. The splicing reactions require snRNPs and accessory proteins that assemble into a splicesome at the intron to be removed.

剪接反应包括两步转酯反应：外显子被连接在一起和内含子以分支的套索结构被释放，此结构含有一个与分支点序列中的保守A残基形成的2'5'键。

Polymorphism of Rb (intron 17) is relatively stable and rarely mutates, but poly morphism of Rb gene (intron 20) locus is characteristic in the old people. The r elation between Rb gene (intron 20) and ageing needs further study.

老年组Rb基因20内含子的位点多态性与青年组比较差异有显著性意义，是否该位点遗传标记与细胞的衰老有一定的相关性，值得进一步研究。

Segments of Adh gene in azuki bean (variety KS 5), covering an interval between partial intron 3 and intron 9, were amplified and sequenced with two pairs of primers. The result has proven the viability of using conserved sequences in a completely sequenced gene of one species to clone same gene in another species. In the comparison between Adh genes of azuki bean and pea, high sequence similarity (about 80%) in exon and long length difference (up to 116 bp) in intron were found. Besides, the edge regions of Adh introns with GT/AG counterparts and more conserved sequences than central regions was observed in both species.

利用两组引子对完成红豆(高雄5号)Adh基因之部分解序，其范围包括部分intron 3至intron 9之间的区域，也印证利用基因之已知保守序列可选殖另一物种之相同基因，红豆与豌豆之Adh基因序列比对结果显示两者之exon均较intron有较高之GC百分比(分别为41.0 ~ 48.7%及23.3 ~ 35.2%)，且两者在exon区域之DNA序列相似程度约80 %，而在intron区域中则有明显的序列差异存在(长度差异最高可达116 bp)，但是intron外侧序列均具有GT/AG组成且相对较中央序列具有保守性。

Intron sequences are removed by RNA splicing that cleaves the RNA at exon-intron boundaries and ligates the ends of the exon sequences together.

RAN 剪接内含子序列由RNA剪接去除，在外显子-内含子的交界处切开RNA，并将外显子的末端连接起来。

All 21 exons with flanking intronic sequences, as well as a 263 bp fragment of the 5" flanking region, of GARS-AIRS-GART gene, were screened in the same five populations, by the method of PCR-SSCP analysis, combined with sequencing reactions. We detected 10 SNPs, C-179T from 5" flanking region, A5669G form exon 3, C6545Afrom exon 4, A7777G from intron 4, G10854T, C10867T, G10898T from intron 6, A16197G from intron 11, C21228T from intron 14, and C29686T from exon 21. These ten polymorphic sites were nominated as GARS1, GARS2, GARS3, GARS4, GARS5, GARS6, GARS7, GARS8, GARS9, and GARS 10, respectively. The mutation of C D A at GARS3 was a non-synonymous mutation, wh

其中外显子4中的6545处的c~A突变导致对应的氨基酸序列中的第173位氨基酸发生变异：天冬氨酸一谷氨酸；10个SNP位点依次命名为：GARSI、GARSZ、GARS3、GARS4、GARSS、GARS6、GARS7、GARSS、GARSg、GARS 10；(2)10个SN'P位点在不同的鸡种中的基因型分布差异显著(P.05)，进一步的方差分析显示，GASRI位点以及GARS3位点与肌肉肌昔酸含量之间存在着显著的关联；(3)在所检测的5个群体中GARsl位

There are different effects of intron in vector on expression of foreign gene cDNA.

在以cDNA表达外源蛋白时，表达载体中是否含有intron会对表达造成一定的影响。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。