查询词典 intracellular

The treatment of leukemic cell with a longterm, intermittent,singular, unefficient chemotherapeutic agents could induce multi-drug resistant cells. Interleukin-4 can reverse the multi-drug resistance by inhibiting the expression of p-gp and increasing the quantity of intracellular chemotherapeutic agents in tumor cells. The effects were probably related to the inhibition of the PKC activity.

长期、间断、单一、不足量的化疗可导致 MDR；IL-4 可通过抑制肿瘤细胞的 p-gp表达，增加化疗药物在肿瘤细胞内的聚集而逆转白血病细胞的MDR，此作用可能与抑制 PKC的活性有关。

Na-K-Exchanging ATPase is a universal cell membrane protein in higher eukaryotic cells which mediates the anti-concentration gradient exchange of intracellular Na+ for extracellular K+. It plays essential metabolic roles such as maintaining sodium and potassium ion gradients across the plasma membrane. The targeted sequences were amplified by PCR to determine the genome structure of the hithertofore unsequenced portion of the alpha 1 subunit of the human Na-KExchanging ATPase gene which encodes 80-130 aa of its extracellular domain using two different templates, human genomic DNA and a human muscle cDNA library. The PCR products were analyzed by restriction endonuclease digestion and then cloned into a plasmid vector for chemiluminescence sequencing and further analysis.

Na-K-Exchanging ATPase是一种普遍存在于高等生物体内的细胞膜蛋白，主要参与介导K+和Na+在细胞内外之间的逆浓度梯度的转运，并维持一定细胞内外的离子梯度，我们采用聚合酶链式反应方法分别以人基因组DNA及cDNA文库为模板对人Na-K-Exchanging ATPaseα1亚单位基因胞外区约80-130位氨基酸编码序列进行扩增，限制性酶切分析扩增产物，并进行荧光测序，对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行进一步的分析，发现人基因组DNA和cDNA经过扩增后分别得到833bp和195bp两种不同大小的片段Fg，Fc。

Cytokines found to have up-regulated in cells were measured in serum and lung tissue of rats exposed to large tidal volume ventilation. For investigating the intracellular pathway of cytokine up-regulation in VILI, exogenous TNF-α or H2O2 was added to culture media of alveolar epithelial cells. Cytokines were then measured. To explore the role of oxidant stress in VILI, N-acetylcysteine, as an anti-oxidant, was used in vitro and in vivo.

我们在体外培养的肺上皮细胞中加入外源性肿瘤坏死因子、过氧化氢或N-乙酰半胱氨酸，观察细胞因子的变化。N-乙酰半胱氨酸亦被投入大鼠呼吸机诱导肺损伤模型，观察用药后细胞因子的变化。

The data obtained from the virus loss experiments showed that the virus retain ability were decreased with long delay duration in the air and viruliferous aphid lost the virus particles by intracellular penetration.

病毒丢失实验结果表明，蚜虫在空气中&滞留&时，持毒能力会不断下降，同时在植物上进行试食穿刺时，病毒主要丢失在植物细胞内。

Further studies are underway to investigate the exact intracellular mechanisms underlying the effects of the two substances. In Chapter 4, we observed the effects of metabolites of mangrove fungus Xylaria sp. from the South China Sea Coast, Xyloketal A～D, on the activity of acetylcholinesterase in vitro, and the present data indicated that AChE activity was remarkably inhibited by Xyloketal A～D in a dose-dependent manner.

另一方面，在论文第3章离体豚鼠回肠收缩实验中，发现323-A和323-B可剂量依赖性地抑制组胺、乙酰胆碱以及氯化钾所引起的GPI收缩，pD2'依次为5.13、4.97、5.36和5.51、5.56、5.62，并且，通过机制分析发现，323-A和323-B可以显著降低在外界无钙离子存在时由细胞内钙储库释放钙所产生的收缩反应，而对由细胞外钙离子内流所引起的收缩无明显影响。

ZHAO Yede*,HE Qinglian.Department of Plastic Surgery, Navy 411 Hospital, Shanghai 200081Objective To investigate the expression level and intracellular distribution of calreticulin in hypertrophic scar-derived fibroblastsand normal skin-derived fibroblasts.

增生性瘢痕(Hypertrophic scar， HS)作创伤及外科手术后常见且十分棘手的皮肤组织疾病，瘢痕组织内成纤维细胞异常活化和细胞外间质过度沉积既是HS的重要组织学特征，也是HS发生、发展的物质基础。

There were five groups in the examination of cellular levelK562 group,K562/A02 group,K562+ADM group,K562/A02+ADM group and K562/A02+TTD+ADM group）.The non-cytotoxicity doses to cell lines K562 and K562/A02 of TTD were got by MTT assay.Using flow cytometry (FCM assay to examine the intracellular ADM concentration.There were three groups in the examination of genic,zymologic and protein levelsK562 group,K562/A02 group and K562/A02+TTD group）.The mRNA expression of MDR was measured by fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR.The expression levels of glutathione-S-transferase and topoisomerase Ⅱ were determined by immunohistochemical technique.

细胞水平检测实验分5组（K562组、K562/A02组、K562+ADM组、K562/A02+ADM组和K562/A02+TTD+ADM组），采用MTT法检测TTD对K562和K562/A02细胞的非细胞毒性剂量，流式细胞术检测细胞内阿霉素的浓度，基因、酶学、蛋白水平检测实验分3组（K562组、K562/A02组和K562/A02+TTD组），采用RT-PCR法检测mdr1 mRNA的表达，免疫细胞化学方法检测谷胱甘肽S转移酶π和拓扑异构酶Ⅱ的表达水平，Western-blotting法检测P-糖蛋白和bcl-2表达。

Objective Fluorescent-labeled antisense oligomer that is hybridized to 18-bp sequences next to the start coden of human proliferating cell nucleus antigen gene was used to assess the intracellular distribution and stability of the oligonucleotides in the presence or absence of liposome.

目的 观察硫代修饰型及天然型PCNA反义寡核苷酸在脂质体介导下或直接转染人膀胱癌细胞BIU-87后在细胞内的分布及其稳定性。

This willlead to acid productions increment,and intracellular acidifying.

糖酵解过程中必然产生乳酸，如果要维持细胞内的糖酵解状态，乳酸必须被转运出细胞外。

The adsorbing rate, utilizing efficiency and intracellular accumulation of sugar were closely related to medium composition.

细胞对糖的吸收速率和利用效率以及胞内糖的积累与培养基的组成有关。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。