查询词典 intracellular

Therefore, the cultivation by combined fed-batch and L-cysteine addition was no doubt one of the most efficient approaches to improve both GSH production and intracellular GSH content, simultaneously.(10) The metabolic network of C. utilis WSH 02-08 for GSH biosynthesis, with glucose as the sole carbon source, was developed based on the stoichiometrical balance equations.

对于分批发酵来说，在L-半胱氨酸添加前后，GSH的合成量约各占总量的50％，对于流加发酵，最终GSH产量可以高达1150mg·L〓，胞内GSH含量达到3.32％，L-半胱氨酸添加后的平均比GSH合成速率更是高出110％。

In particular, if they could enter the cell, the process of internalization, their localization in intracellular compartments, and their succedent effects on the cellular functions, the current findings for these aspects are summarized in this paper.

尤其是这类纳米颗粒是否能够进入细胞，如何进入细胞，在细胞内的定位，以及对细胞的生物学功能有何影响，本文对这些问题，从不同的方面进行了综述和探讨。

The extracts of extracellular and intracellular insecticidal proteins from Xenorhabdus nematophila HB310 strain, an entomopathogenic bacterium symbiotically associated with entomopathogenic nematode Steinernema carpocapsae, appeared high toxic activity by oral or injectable infection against several species of insects.

嗜线虫致病杆菌Xenorhabdus nematophila HB310是从河北省土壤中筛选出的一株昆虫病原线虫体内分离纯化获得的共生菌，该菌的发酵液对多种昆虫有较高的杀虫活性。

We tested whether inhibitory GABAergic synaptic transmission regulates Xenopus optic tectal cell dendritic arbor development in vivo by expressing a peptide corresponding to an intracellular loop ofthe gamma2 subunit of type A GABA receptors GABA(AR, which is required to anchor GABA receptors to the postsynaptic scaffold.

我们想证明如下设想：抑制性GABA能突触传递是否能调节早蟾视顶盖细胞的树突发育。因为GABA受体的gamma2亚基有一段细胞内的跨膜端序列，这段序列对锚定GABAA受体到突触后膜是必须的，所以我们的主要方法是设计了一段特异性的争对细胞内段的序列，这样就特异性的阻断了单个细胞GABA能的突触传递。

Primary HMC were cultured and passage 3 and passage 4 of the cells were used. Intracellular calcium release was assayed with confocal analysis. Expression of TGF-β mRNA and the content of supernatant fibronectin were teste...

结果 ：IgA肾病患者aIgA1呈时间依赖性诱导正常HMC细胞内游离Ca2 +释放、TGF -βmRNA表达和Fn分泌，作用趋势与正常人aIgA1相同，高峰时间分别为孵育后 6 0s、2 4 - 36h和36 - 4 8h ，但作用强度显著高于正常人aIgA1；在作用高峰时间，患者组和正常人组Fluo - 3最大相对荧光强度增加值分别为(95 83± 11 4 3)和(5 5 88± 12 72 )，TGF -β/GAPDH的比值分别为 0 96± 0 0 6和 0 74± 0 0 2 ，Fn含量分别为(6 4 5± 0 18)μg/L和 5 5 4± 0 。。。

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2～3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12，在酵母细胞中实现了多拷贝强表达；荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液，测定510 nm处的荧光发射强度结果显示，表达的绿色荧光蛋白对氧化还原水平敏感，且在510 nm处的荧光强度与一定的重金属浓度呈正相关，即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强，从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用；同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属；遗传学的点突变频率及致死率实验数据表明，重金属能导致菌体的点突变频率和致死率升高，且活性氧的清除剂巯基脲能明显降低这种点突变和致死率，说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

Objective To investigate the effect of ligustrazine on the intracellular translocation of Smad protein in HSC-T6 cell line.

目的 探讨川芎嗪对HSC-T6细胞Smad蛋白细胞内转位的影响。

ERCS can inhibti intracellular signal transudation of PDGF in HSC to block the biological effect of PDGF.

莪术提取物药物血清能抑制PDGF诱导的肝星状细胞内Ca(上标 2+)内流及PI3-K的表达。

Over-expression of NaDC3 accelerates the speed rate of energy metabolism and increases intracellular ROS generation by transporting an overdose of tricarboxylic acid cycle intermediates in human renal tubular epithelial cells.

NaDC3过表达可通过转运过多的三羧酸循环中间代谢产物进入细胞内而加快肾小管上皮细胞能量代谢的速率、增加细胞内ROS的产生水平。

RESULTS Specific inhibitors of respiratory chain complexes Ⅰ,Ⅱ,Ⅲ, and Ⅳ of rotenone, thenoyltrifluoroacetone, antimycin A and potassium cyanide, and the mitochondrial uncoupler carbonyl cyanide mchlorophenylhydrazone resulted in obvious changes of intracellular ROS production.

结果 呼吸链复合物Ⅰ，Ⅱ，Ⅲ，和Ⅳ抑制剂鱼藤酮、噻吩甲酰三氟丙酮、抗霉素A、氰化钾及氧化磷酸化解偶联剂羰氰苯腙能够明显改变But所致细胞内活性氧的产生。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。