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METHODS The HCV core gene coding region was inserted into the eukaryotic expressive plasmid pcDNA3, then the recombinant plasmid pcDNAHCV-C was constructed and expressed transiently with LipofectAMINE in the SP2/0 cells. After purification, these plasmid directly or encapsuled wit h Lipofect-AMINE were injected into BALB/c mice. HCV core antibody from immunized mice could be detected by ELISA. RESULTS The enzyme -cutting identification showed that HCV core gene fragment had been cloned into pcDNA3 eukaryote vectors.

人工构建包含HCV C基因片段的真核表达载体pcDNAHCV-C，在证实其可以在真核细胞中表达之后，将其用脂质转染剂包裹，形成LipofectAMINE-pcDNAHCV-C脂质混合物，将该混合物或单纯pcDNAHCV-C直接注射BALB/c小鼠股四头肌，以空载体pcDNA3做对照，ELISA法检测血清中抗体产生水平。

The author tries to review the individualized composition teaching guidedby the new curriculum based on current research and the author's seventeen years of experience in teaching Chinese, carries on historical introspection into the long lost "individualization" of composition teaching, tries to probe into the concept of composition teaching deeply, and explains the unique understanding of the individualized composition teaching, thecharacteristics of individualized composition teaching, the view of teaching and viewofrelationshipbetweenteachers and students in individualized composition teaching as well asthe requisition for Chinese teacher's accomplishments so as to probe into the way ofimplementing individualized composition teaching and the procedure of training, and tries toavoid the tendency of generalized and formularized traditional composition teaching.

个性化作文教学是语文教育理论和实践关注的&热点&问题之一，是社会发展和教育改革的产物，是新课程标准指导下的作文教学新理念。本文试图在现有研究的基础上，在新课程标准的背景下，结合自己十年来的语文教育实践，对个性化作文教学的问题进行反思，深入探讨个性化作文教学理念，阐释对个性化作文教学的独特理解，包括个性化作文的内涵，个性化作文的特征，个性化作文教学观、师生观，尤其强调创造性思维、和创作的主体性在个性化作文教学中的地位，同时建立新的作文评价体系等，旨在探索赋予个性的作文教学的培养方法，落实个性化作文教学的训练环节及实施途径。

By using a mean field approximation, the process of lthium intercalation into carbon materials was analyzed theoretically. GICs with less than 4 stage form when lithium intercalates into graphite by electrochemical methods. The capacity of lithium intercalating into carbon materials depends on the energy dispersion of lithium site in carbon.

通过平均场近似从理论角度分析了锂在石墨材料和碳素材料中的电化学插入过程，结果表明：锂插入到石墨中一般只形成4阶以下插层化合物，容量可达到LiC〓；而在碳中锂的插入量，根据锂可插入位置能量分布不同，一般小于锂在石墨中的插入量。

METHODS: Acetic acid solution was added into chitosan to prepare 8% chitosan acetic acid hydrosol, which was then stayed for one day. Flowing the immersion into the hydrosol and immediate dislodgement, plastic tube was soaked in 1 mol/L sodium hydroxide for 3 minutes. Hydrosol out of the tube etiolated and coagulated into jells, the tube was removed when the jells were semi-dried.

利用乙酸溶液制备8%壳聚糖乙酸水溶胶，溶胶放置1 d，将硬塑料管浸入溶胶后迅速取出放入1 mol/L氢氧化钠液内3 min，见管外溶胶变白凝固成胶冻状，取出后晾到五分干后轻轻退下内管，晾干后即成为壳聚糖导管。

Our state animal remedy producer has increased into 2600 in 2004 from tens in 1985, also the animal remedy market fast changed from seller's market into the buyer's market in middle period of 1990's, at the beginning of this century, the competition for market was most bitterly, the whole industry come into a period of a narrow margin profit.

我国兽药兽药企业从85年的几十家发展到2004年的2600家，从90年代中期前的卖方市场迅速演变为买方市场，到本世纪初市场竞争已进入最激烈的状态，整个行业进入薄利阶段。

The external mechanism of check turns in the people's interests into the internal check criterion, strengthens the collection of the information, set correct direction for the internal check; The internal mechanism of check can have the ex post check back-inferring to the ex ante and inter check; change the indirect check pointing to the thing but not the person into the direct one that points to the person but not the thing; having the check for the whole work of public authority department broken up and numerated by changing it into the individual's check.

外部考评将人民群众的利益内化为内部考评标准，强化内部考评信息采集，为内部考评机制提供正确的导向；内部考评机制将外部考评的事后考评倒推到事中和事前考评；将对物不对人的间接考评转化为对人不对物的直接考评；将对公共权力部门整体工作的考评分解量化为对个体工作的考评。

During the etenal wrangling and wars that make up the great game,it came about that tzeentch tricked slaanesh into battling with khorne.the changer of ways lured slaanesh into a cause he would not archieve,and slaanesh was defeated and humiliated.seeing the dark mood of her master,the masque took it upon herself to ease his heart with her most energetic and scintillating dance ever.where once her leaps and pirouettes had brought laughter and joy, now slaanesh's bitter heart saw mockery, each perfect combination of move calculated to be barbs into his pierced pride.slaanesh cast the masque aside,condemning her as a traitor.he cursed her,saying that if she wanted to dance,she must dance forever more.

在那被叫做大游戏(#注1)的无尽的冲突与战争中，曾有一次，tzeentch用计挑拨slaanesh与khorne开战。道路的篡改者引诱slaanesh陷入了他没有预料到的因果中，而slaanesh耻辱的战败了。看到她主人阴暗的情绪，假面舞女认为自己有责任用她最闪烁激情的舞蹈来抹去他心中的不快。但是当她的跳跃和旋转开始展现欢笑与享受的时候，slaanesh苦涩的心却只看到了嘲笑，每一个精确调整的完美的动作组合，都在刺痛着他的被伤害的骄傲与自大。slaanesh喝止了假面舞女的舞蹈，如叛徒般的废除了她的地位。他诅咒她，说道&既然你想跳舞，那么你会永远这样跳下去&

During the etenal wrangling and wars that make up the great game,it came about that tzeentch tricked slaanesh into battling with khorne.the changer of ways lured slaanesh into a cause he would not archieve,and slaanesh was defeated and humiliated.seeing the dark mood of her master,the masque took it upon herself to ease his heart with her most energetic and scintillating dance ever.where once her leaps and pirouettes had brought laughter and joy, now slaanesh's bitter heart saw mockery, each perfect combination of move calculated to be barbs into his pierced pride.slaanesh cast the masque aside,condemning her as a traitor.he cursed her,saying that if she wanted to dance,she must dance forever more.

在那被叫做大游戏(#注1)的无尽的冲突与战争中，曾有一次，tzeentch用计挑拨slaanesh与khorne开战。道路的篡改者引诱slaanesh陷入了他不可能实现的目标中，而slaanesh耻辱的战败了。看到她主人阴暗的情绪，假面舞女认为自己有责任用她最闪烁激情的舞蹈来抹去他心中的不快。但是当她的跳跃和旋转开始展现欢笑与享受的时候，slaanesh苦涩的心却只看到了讽刺，每一个精确调整的完美的动作组合，都在刺痛着他的被伤害的骄傲与自大。slaanesh喝止了假面舞女的舞蹈，如同对待叛徒般的废除了她的地位。他诅咒她，说道&既然你这么喜欢跳舞，那么你会永远这样跳下去&

Sixty-two artificially respired rabbits were anesthetized with urethae.Intranuclear microinjection was adopted.Microinjection of TRH into Lc-Sc induced a marked increase in respiratory frequency and significant elevation of blood pressure.The above mentioned effects of TRH into Lc-Sc on RF could be blocked by preinjection of α2 or β receptor antagonist into RVLM respectively.

实验选用62只行乌拉坦静脉麻醉和人工呼吸的家兔，采用脑内核团微量注射的方法，将TRH微量注入蓝斑复合核，使呼吸频率明显增快，平均动脉血压升高；在延髓头端腹外侧区分别预注射α2或β受体阻断剂，使TRH在蓝斑复合核的呼吸增频效应明显减弱，其升压效应可被延髓头端腹外侧区分别预注射α或β受体阻断剂拮抗。

Medical grade SiO was injected into cat eyes after gas-compression vitrectomy. Balanced salt solution was injected into the other eyes as a control. All cats were examined with biomicroscopy and ophthalmoscope pre and postoperatively; intraocular pressure was also measured regularly. After 1, 3 or 6 months of cat's survival, the morphology of RGCs labeled with retrograded HRP injected into the bilateral lateral geniculate nucleus and reacted with diaminobenzidine was quantitatively analyzed.

30只健康成年家猫，气体玻璃体切割后实验眼注入医用硅油，对照眼注入平衡盐溶液，术后定期眼部检查并监测眼压。1、3、6月后进行形态学实验：（1）微电极定位双侧外侧膝状体并注入辣根过氧化物酶，48～60小时后灌注固定，剥取视网膜行二甲基联苯胺成色反应；（2）视网膜尼氏染色；（3）眼球矢状切片常规HE染色。

" I' d like to get some rough idea about music in the baroque ear, please."

请简要介绍一下巴罗克时期的音乐，好吗？

The results showed that the peak latency and pattern of SEPs elicited by electrical needling in LI-l1 and MP were similar. The amplitude of SEPs elicited by electrical needling in LI-l1 was higher than that of MP. There was no obvious SEPs generation when MM was electrical needling.

结果显示，电针刺激曲池穴和其邻近桡侧伸腕长肌运动点诱发性脑电波的波形和波峰时间是类似的，只是穴位的诱发性脑电波波幅较大，而电针刺激非穴位点并没有明显的诱发性脑电位。