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Song:The other side Artist:Sirenia There's a light and a darkened road There's a night and a fading hope There was a dream that once was mine But now it seems it has passed with time Sail away my little sister Sail away to the other side Sail away my little sister Sail away far into the night Where times seems much better Than this void called life There's a voice inside my head There was a hope,now long since dead It's all a wonder, will I abide I hear you calling from the other side I hear you calling from the other side Sail away my little sister Sail away to the other side Sail away my little sister Sail away far into the night Where times seems much better Than this void called life ----chorus------- I hear you calling from the other side Sail away my little sister Sail away to the other side Sail away my little sister Sail away far into the night Where times seems much better Than this void called life
宋:另一方演出者:海牛目有一盏灯,黑暗的道路有一个夜晚和一个衰落的希望有一个梦想,曾经是我的但现在看来,它与时间的推移航行距离我的妹妹航行距离对方航行距离我的妹妹航行距离远到深夜凡时代似乎要好得多超过这一空白称之为生命有一个声音在我的头有一个希望,现在早就死了这都是一个奇迹,我会遵守我听说你从对方要求我听说你从对方要求航行距离我的妹妹航行距离对方航行距离我的妹妹航行距离远到深夜凡时代似乎要好得多超过这一空白称之为生命----合唱-------我听说你从对方要求航行距离我的妹妹航行距离对方航行距离我的妹妹航行距离远到深夜凡时代似乎要好得多超过这一空白称之为生命
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Because of my this hobby, I tendentiously put Chinese handicraft into my design, but what I love more is to change these fine arts into three-dimensional space installation and change it into a part of the space.
因为这个爱好,很容易便把一些工艺美术融入我的设计,但我更喜欢的是把这美术变成立体的空间装置,把它变成空间的一部分,可能这种对文化艺术的重新演绎,赋予了它现代的气息。
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During this research, many persons become the object of study. Investigation is conducted with 200 Grade One senior school students who are from No.1 Middle School of GanZhou, Yudu Middle School and No.1 Middle School of Yichun. 50 excellent teachers and 9 experts. At the same time, I made a more meticulous analysis by making researches into the exam questions of Jiangxi Senior School Entrance Examination and particularly making researches into the chemistry exam of experimental zone in 2005. At last, I have found the scientific methods, techniques and artistry of creating items by making researches into more than one hundred and twenty papers what are from the whole countrys experimental and unexperimental zones in recent years.
在研究过程中,本人以江西省赣州一中、于都中学以及宜春一中2005级200名高一学生,2006年参加中考研讨会的部分省市骨干教师50名以及9名九年级化学内容专家为调查对象;同时以江西省中考化学试题特别是江西省课改实验区2005年中考化学试题为案例;对全国各省市课改实验区及部分非课改实验区的近三年来的一百二十多份试卷的分析,以发现试题命制的科学性、技巧性和艺术性等。
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Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.
参考已发表的ChIL-15基因序列,设计合成引物,应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA,将其与SmalⅠ酶切处理的pUC19载体连接,构建重组质粒pUC19ChIL-15;用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因,与pMD 18-T载体相连构建重组质粒pMDChIL-15;然后用KpnⅠ/PstⅠ双酶切下mChIL-15基因片段,定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中,构建重组质粒pP_RoChIL-15,对其测序确认读框正确后,转入大肠杆菌DH5α中诱导表达并进行纯化,用重组ChIL-15(rChIL-15)免疫豚鼠,制备多克隆抗体;再用HindⅢ/PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因,定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中,经酶切、PCR和序列测定鉴定后,与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞,经三轮蚀斑纯化,构建重组杆状病毒rBac-ChIL-15,用该重组病毒感染处于对数生长期的Sf9细胞,按不同的时间收取样品,离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。
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Magic is the power to change – to change matter into energy, unliving into living, lead into gold, and the opposite of all these.
魔法是变换之力—能将物质转化为能量,起死回生,点石成金,或其相反。
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For strong convective weather , it classified all historical sample events into 4 weather patterns (like northwest , trough area ,west wind and southwest current), established 4 characteristic fields of 400hPa height of HLAFS , then according to the principle of the pattern match calculate and compare the real-time HLAFS forecast products-2-using the similar method inside the big and small key areas , establish forecast equation , finally gain conclusion .To duststrom weather, the historical samples were sorted into duststrom and severe duststrom types in 5 regions (they are whole area, west, central, middle-west and middle-east of Inner Mongolia ),moreover set up different sample databases about ECMWF fields (including 500hPa height, 850hPatemperature and sea-level pressure).In order to making duststrom forecast at different time level , we first filtered the real-time data by FAX data ,and then used the method of similar range degree to compare the historical data to the actual data of ECMWF .To precipitation weather ,they were divided into 2 types that suit or unsuit airplane artificial precipitation stimulation in line with their emergence time and district .The appropriate weather physical factors come from T106 were chosen to establish the artificial precipitation stimulation prediction model . In the actual application , we can get prediction result as long as use the real-time forecast data of T213 in the prediction model .
针对强对流天气将历史样本天气分为西北气流型、槽区型、西风气流型和西南气流型等4个类型,并建立其HLAFS资料400hPa四种特征场,按照模板匹配的原理,用相似分析方法在大、小两个关键区中对实时HLAFS预报产品进行计算、比较,再建立预报方程,得出结论;对沙尘暴天气将其按区域分为全区、西部、中部、中西部以及中东部5类,再按强弱分别分为强和一般2类,建立ECMWF的3个场(500hPa高度场、850hPa温度场和海平面气压场)的历史资料库,在用传真资料消空之后,用相似离度方法计算实时ECMWF资料,做出不同时次的预报;对降水天气按出现时间和区域分为适合和不适合飞机增雨作业2类,选择T106资料中恰当的气象物理量因子,用BP神经网络算法建立人工增雨降水预报模型,实际应用中将实时T213相关预报资料代入预报模型即可。
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The changes include:(1) Put the urediniospores into the tube with two steel balls and 1. 6 g/L Chelex-100,the uredinia may be picked up with airing host tissues.(2) Add KOH into the extraction buffer instead of NaOH and vortex the tube violently for 30 min.(3) Prick a hole under the bottom instead of picking out of the steel balls, then centrifuge the tube to toss the liquid into the sheathed tube.
改进的 内容包括:(1)在有二钢珠和1.6 g/L Chelex的离心管中加入夏孢子,夏孢子可以来自带有风干寄主叶肉组织的夏孢子堆;(2)在离心管中加入KOH而不是NaOH,然后剧烈斡旋30 min;(3)将离心管管底穿一小孔,套上一新离心管,短暂离心甩出内容物。
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Hypoxic-ischemic encephalopathy experimental grouping: Sixteen successful C57/BL6J mice models, aged 7 days, were randomized into 2 groups: experimental group (n =8), following ligation of right common carotid artery, mice were placed in the closed container containing 0.08 volume fraction of oxygen and 0.92 volume fraction of Nitrogen gas, and taken out 1.5 hours later; 3 μL ESCs were injected into the right cerebral ventricle at about 1 week, and control group (n =8), in which, the same amount of PBS was injected into the right cerebral ventricle.③ At 12 weeks after transplantation, the survival and migration of induced ESCs labeled by Lac-Z in the spinal cord and brain were observed by zymologic method.
缺氧缺血性脑病实验分组:将造模成功的鼠龄7 d的C57/BL6J16只小鼠随机分为2组:实验组8只,结扎右侧颈总动脉后将小鼠置入含体积分数0.08氧气和体积分数0.92氮气的密闭容器内,1.5 h后取出;1周后在右侧脑室内注入3 μL诱导胚胎干细胞。
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Take a container into dried meat, onions, ginger, dried shrimps at the end of carrageenan meters at the end of Chinese cabbage, parsley at the end, adding pepper, monosodium glutamate, salt, stirring uniform, made of 3.3 cm in diameter of the ball, ping On the plate, Wang Huo steamed into the cage with about 8 minutes until cooked out on the bowl, with Tang Guo Add broth, salt, steam boiling, put parsley paragraph Wanghuo boil after adding MSG, into large soup Bowl, applying sesame oil, vinegar, pepper sprinkled chunks.
取盛器一个,放入瘦肉茸,葱、姜末、海米末、鹿角菜米、大白菜末、香菜末,加入胡椒粉、味精、精盐,搅拌均匀,制成直径3.3厘米的丸子,平摆在盘内,入笼用旺火蒸约8分钟,至熟取出,放在大碗内,汤锅内放入清汤、精盐,蒸沸,放香菜段旺火烧开后加入味精,倒入大汤碗中,淋上芝麻油,食醋,撒上胡椒面即成。
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When we have come into high enough resonance with the frequency patterns of the Divine Names within our very own beingness, the pathway is then opened for the angelic beings holding these patterns, to project their image directly into your holistic body / mind / soul template, thereby bringing you the last distance across the upper threshold into your divine heritage: full expression_r of the Adam Kadmon template.
当我们拥有了浸淫非常自我化属性的神性名称频率模式进入到足够高的共振时候,于是持有这些特质的天使生命这样的道路就开启了,就可以投射天使的图像进入你全部的身体/思想/灵魂模板内去,因此把你最终的越过上面的道路带进到神性传承里来:这就是亚当卡德蒙模板的完整解释。
- 相关中文对照歌词
- I'm So Into You
- Down Into Muddy Water
- Let Me Put My Love Into You
- Into The Night Of Blue
- Into The Fire
- Born Into A Light
- Breakdown Into The Resolve
- Step Into The Light
- Falling Into Place
- Turn Those Clapping Hands Into Angry Balled Fists
- 推荐网络例句
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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.
然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。
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Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.
镀锌钢托盘多用于出口,替代木托盘,免薰蒸,符合欧盟、北美各国对出口货物包装材料的法令要求;喷涂钢托盘适用于重载上货架之用,托盘表面根据需要制作成平板状、波纹状及间隔形式,满足不同的使用要求。
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A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.
付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。