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METHODS: Rat bilateral olfactory bulbs and olfactory mucosa at 1/3 nasal septum were obtained, sliced, digested in trypsin, and made into monoplast suspension. At 1×109/L, cells were incubated in uncoated 25 cm2 culture flask. At 18-20 hours, cell suspension was moved into another uncoated 25 cm2 culture flask (the first differential adhesion). At 24 hours, cell suspension was moved into a poly-D-lysine-coated 25 cm2 culture flask or poly-D-lysine-coated 6-well culture plate (the second differential adhesion). At 48 hours, parenchyma cells were removed after a half of medium was changed.

完整取大鼠双侧嗅球及剪取鼻中隔后1/3嗅黏膜，剪碎后胰酶消化，制成单细胞悬液，按1×109 L-1密度接种于未包被的25 cm2玻璃培养瓶中，18~20 h后将细胞悬液转移至另一未包被的25 cm 2玻璃培养瓶中（第1次差速贴壁），24 h后再将细胞悬液转移至经多聚右旋赖氨酸包被的25 cm2塑料培养瓶或经多聚右旋赖氨酸包被的6孔培养板中（第2次差速贴壁），接种培养48 h后半量换液以去除杂细胞。

Methods: 64 cases with multiloculated pleural effusions were divided into 2 groups, the treatment group with 34 cases was treated with urokinase,injecting 100000～200000u/time and ns 10～20ml into pleural cavity;the control group with 30 cases was treated with chymotrypsin,injecting 100000～150000u/time and ns 10～20ml into pleural cavity.results: after treated with urokinase or chymotrypsin.

配合病因治疗，对64例有小房形成的胸腔积液随机分2组，治疗组34例胸腔内注射生理盐水10～20ml+尿激酶10～20万单位；对照组30例给予生理盐水10～20ml+糜蛋白酶10～15万单位。

Methods 100 cases were randomized into treatment group in which 50 cases were treated with Safflower injection via carotid artery infusion, added Safflower injection 20ml into 0.9% sodium chloride injection 60ml with the 4ml/min velocity, once a day; control group in which 50 cases were treated with venous drip of Safflower injection, added Safflower injection 20ml into 0.9% sodium chloride injection 500ml. After therapy, the curative effect、 myodynamia and hemorrheology were compared between the two groups.

将100例脑梗死患者随机分为红花注射液颈动脉灌注组50例和红花注射液静脉滴注组50例，治疗组：于0.9%氯化钠注射液60ml中加入红花注射液20ml，以4ml/min的滴速经颈动脉灌注；对照组：以红花注射液20ml加入0.9%氯化钠注射液500ml中静滴，20天后对两组疗效进行分析并观察治疗前后肢体肌力及血液流变学变化。

The intermetallic compounds are coarsened into nubbly Cu6 Sn5 phase by adding excessive Cu into Sn-Cu solder alloy. For Sn-9Zn-xCu solder alloys, the long needle-like Zn rich phase which is the mainly existing form of Zn in Sn-Zn solders is transformed into Cu5 Zn8 phase and fine Zn rich phase by the addition of 2% Cu, while CuZn phase and Cu6 Sn5 phase are formed in Sn-9Zn-10Cu bulk alloy. When soldering within short time under 260℃, the ripening and growth of IMC at Sn-xCu/Cu interface are accelerated with increasing Cu content in Sn-Cu solders.

结果表明，当Sn-Cu钎料中Cu的含量为2%时，基体中IMC粗化为块状的Cu6Sn8相；对于Sn-9Zn-xCu钎料合金，2%Cu元素的加入使得Sn-9Zn基体中长针状的富Zn相转化为Cu5Zn8相以及细小的富Zn相，而当Cu含量达到10％时，钎料基体中的IMC为CuZn相与Cu6Sn5相。

RESULTS: The time of misplacement of pedicle screw was 5-69 days with an average time of 18.5 days, including 7 cases of screw penetrating into lateral cortex, 4 of screw penetrating into medial cortex, 2 of screw penetrating into pedicle cortex, 2 of overplacement, 2 of intervertebral foramen placement and 2 of intervertebral space placement.

结果：患者自螺钉置入后至发现椎弓根螺钉误置时间为5~69 d，平均18.5 d。其中螺钉穿破椎弓根外侧皮质7例，穿破椎弓根内侧皮质4例，螺钉穿破椎弓根皮质2例，螺钉置入过深2例，螺钉进入椎间孔2例，进入椎间隙2例。

All cases were invasive-7 into at least the lamina propria, 7 into at least the muscularis propria, and 3 into perivesical fat.

所有病例都具侵袭性，至少侵袭至固有膜和固有肌层的各有7例，3例侵袭至膀胱周边脂肪组织。

In the presence of L-isoproterenol (through local infusion into area CA1), the theta-pulse stimulation with the parameter of 10 Hz, 150 pulses/train, 1 train, a frequency weakly modifying synaptic strength, induced a robust LTP, and this effect was blocked when DL-propranolol was co-administered. By contrast, the theta-pulse stimulation with the parameter of 5 Hz, 150 pulses/train, 3 train, a frequency strongly modifying synaptic strength, induced a significantly smaller LTP when DL-propranolol was administered into area CA1. Accordingly, DL-propranolol impaired rat's spatial learning in the water maze when infused into area CA1 20min pretraining.

正常情况下对突触传递效能仅有微小调制作用的10Hz的θ节律刺激（每串150个脉冲，1串），在CA1区局部给予L-异丙肾上腺素后，显著地诱导出LTP，这一效应可被DL-心得安所阻断；相反，正常情况下对突触传递强度有显著调制作用的5Hz的θ节律刺激（每串150个脉冲，3串），在CA1区局部给予DL-心得安后，所诱导出的LTP被显著压抑。

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

The distance between the skin which the needle stab into and the ligamentum, is (72.2±0.2)mm and between the skin which the needle stab into and the pudenda nerve is (80.2±0.1 ) mm. Conclusion: We should have a specific depth and angle when acupounct into the acupoint of Zhenyang, to make it sure to get the specific feeling of acupunting. The main reason of getting the specific feeling of acupunting is the needle may touch the pudendal nerve.

针刺&振阳穴&产生特殊针感可能是由于直接刺激到了阴部神经，或由于针刺时引起局部肌肉组织收缩牵拉刺激阴部神经所产生的，治疗阳痿的作用机制可能是通过调节大脑皮质和脊髓节段反射来影响阴茎勃起功能的。

Our effort is on the base of TOSHIBA 1MR—15 linear accelerator and PH- ILIPS OSS radiotherapeutic plan system,put the data of American Varian therapy device into O SS system,then merge the data of LMR—15 and Varian two kind of therapy devices into a DIF document,put it into OSS system,so reach the function that OSS system surports two kind of therapy devices at the same time.

本工作是在东芝LMR—15直线加速器和菲利浦公司的OSS放射治疗计划系统的基础上，将美国Varian治疗机数据装入OSS系统，进而将LMR—15和Varian两种治疗机的数据合并为一个DIF文件，装入到OSS系统中，实现了OSS系统同时支持两种治疗机的功能。

As of Tuesday, Google's results were still censored in China.

截至周二，谷歌的搜索结果仍受中国审查。

In order to make the positive action increase and negative one decrease, the sub-forces of the social factors must be adjusted to form a centripetal force.

在这一过程中，人的主体性发挥是社会有机体健康发展的灵魂。