查询词典 intersecting lines

They are trying to turn the constitutional vote into a referendum on Mr Morales and his attempt to "refound" Bolivia along "indigenous" and socialist lines.

莫拉莱斯试图从&原住民&和走社会主义道路来&重建&玻利维亚，官员也就想把这场修宪公投变成对莫拉莱斯及其设想的全民公投。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Methods Compounds were isolated and purified by various column chromatographic techniques and their structures were elucidated on the basis of spectral analysis (IR, ESIMS, 1HNMR, 13CNMR). The inhibitory activity against the proliferation of HL60, REH and Raji cell lines was determined by using MTT assay.

用色谱方法分离栀子中化学成分，用波谱技术(IR，ESIMS，1HNMR，13CNMR)进行结构鉴定，用四唑盐比色法测定化合物对白血病细胞（HL60人早幼粒细胞白血病、REH人急性淋巴细胞白血病、 Raji淋巴瘤）增殖的影响。

She is relishing the day the new subway lines are connected, slicing even more money and time off her journey.

她回味新的地铁线连通的今天，她的旅途变得更加节省金钱和时间。

To minimize cellular variations, we generated stable cell lines for firefly luciferase reporters, and used Renilla luciferase-fused transcription factor to measure the quantity of individual transcription factor.

为了将细胞的变化值减到最小，我们设计了带有firefly luciferase reporters稳定细胞株的建立，并且利用fusion protein(同时带有Renilla luciferase与转录因子构筑融合蛋白)去计算出单独转录因子的数量。

Objective: To understand the differentially gene expressions of nasopharyngeal carcinoma CNE-2 cell lines in different proliferative status and to investigate the mechanism of accelerated repopulation in NPC during irradiation.

目的：了解不同增殖状态鼻咽癌细胞（CNE-2）细胞周期基因表达差异，探讨鼻咽癌放射治疗中加速再增殖的机理。

They could not break the gray lines by direct assault and so, under cover of night, they marched through the mountain passes in a semicircle, hoping to come upon Johnston's rear and cut the railroad behind him at Resaca, fifteen miles below Dalton.

最后北方佬不得不退回另作商量了。他们无法从正面突破南军的防线，便在夜幕掩盖下迂回越过山隘，想走到约翰斯顿的背后切断雷萨卡以南15英里处的铁路。

The spray system locates different types of sprays spatially to develop three lines of defense to minimize respirable dust emissions in and around continuous miner face areas.

喷雾系统位于不同类型的喷雾空间发展三道防线，尽量减少呼吸性粉尘的排放量和周围连续采煤机面对地区。

Objective To study the differential display of promyletic leukemia cell lines induced and non-induced by all-trans retinoid acid.

摘 要］目的对经全反维甲酸诱导和未诱导的HL-60细胞差异表达基因进行初探。

As the field is rewoven to have proper serpents holding the positive and negative lay lines of the field and form, the machines are no longer necessary and can be dismantled.

当场被重织，让适当的蛇掌握场和形态的正极和负极层面线，机械不再需要，且能被拆除。

More direct, directer ; most direct, directest

径直的，笔直的

Do you know how to use a chain saw?

你知道如何使用链锯吗？