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A steel bar is washed and dried, and then put into a glass pipe which is sealed to obtain stirring magneton; a mixed solvent of Gamma-methacrylate oxide propyl trimethoxy silane and methanol is prepared and added with an organic base to obtain a pre-bonding solvent; the stirring magneton is immerged into the pre-bounding solvent, and the outer surface of the glass pipe of the stirring magneton is bonded with a layer of silane substance with alkenyl; a reactive monomer mixture and a pore-forming agent mixed solvent are evenly mixed and then added into a container, the stirring magneton after the pre-bounding is put into the container which is sealed and then put into a water bath; after reaction, the container is removed, the stirring magneton coated with a polymer is taken out and put into a extractor and extracted by using a solvent; or the stir bar is immersed into the solvent till no impurity can be checked in the solvent, thereby obtaining the product.

将铁条清洗干燥，放入玻璃管内，密封得搅拌磁子；配制γ-甲基丙烯酸氧丙基三甲氧基硅烷与甲醇的混合溶液，加入有机碱得预键合溶液，将搅拌磁子浸入预键合溶液，在搅拌磁子的玻璃管外壁键合一层带烯基的硅烷化物质；将反应单体混合剂和致孔剂混合溶液混匀后倒入容器中，将预键合后的搅拌磁子放入容器，密封后置于水浴中，反应后除去容器，取出涂有聚合物的搅拌磁子，置于提取器中，以溶剂提取，或将搅拌棒置于溶剂中浸泡至溶剂中无杂质检出，得产物。

Dis- Fly away lonely souls into the endless sky so far away When you're all alone Feeling down and lonely Tell me what you see deep inside your soul You only know who you are So don't be afraid Just open your heart to me You will spread your wings like an eagle Learning how to fly in the sky Just like the sun rises after the rain you'll shine in the air Take off your mask and be true to your heart trust yourself and fly away Into the light of joy and truth Where there's a grief there will always be a hope For a place where we can be who we wanna be Your tears may fade away But always keep the faith and live tomorrow Break through the night away into a brand new day Life's just begun Yeah, feelin' the melody of eternity It has the power to set us free Travlin' through the galaxy with only space around Follow the suns peaceful sound It's time for us to end this illusion Fightin' ourselves will only cause confusion Shinin' light is the key of life Let's all get together and make this better I don't know how but there has got to be a way There are times when I Wish I could just disapear Far from this place of pain and hurt If only I could be gone There's something in your eyes Pulls me back from running away Oh, yes it's hard sometimes But now I'm strong enough to tell the sadness goodbye Just like the moon fills the night with the stars I'll dance in the air Seek in the darkness there's nothing to fear I will stand and fly away Into the night I'll find the truth The pain never ends but there's always be a hope To be free eternally in this galaxy To live another day the sun will show the way and I will follow Break through the night away into a brand new day My journey goes on Into the light of joy and truth Where there's a grief there will always be a hope For a place where we can be who we want to be Your tears may fade away But always keep the faith and live tomorrow Break through the night away into a brand new day Your journey's begun

阴间的神-飞孤单的灵魂进入在如此远处的永无止境的天空之远之内当你全部很孤独的时候感觉下来和孤单的告诉我你在你的灵魂里见到起来深的你只知道你是谁如此不害怕仅仅对我开放你的心你将会像一只鹰一样传布你的翅膀学问该如何在天空中飞仅仅在雨之后喜欢太阳上升你将会在空气中发亮脱掉你的假面具而且忠于你的心信赖你自己和飞行之远进入欢喜和事实的光之内哪里总是有伤心事在那里意志是一个希望对于我们能是的一个地方我们想要当谁你的泪滴可能褪色离开但是总是保存信心和活的明天进入商标新的日子之内夜晚之久突破仅仅被开始的生活是的，feelin'永恒的曲子资讯科技有力量释放我们 Travlin'经过银河由于只在附近隔开跟随太阳和平的声音资讯科技是我们结束这一个幻影的时候 Fightin'我们自己只将会引起混乱 Shinin'光是生活的钥匙全部让我们聚在一起而且使这变成更好我不知道如何除了那里之外已经开始是一个方法有时代此时我希望我可以正直的 disapear 离痛苦和伤害的这一个地方很远如果只有我可能不见了某事在你的眼睛中从逃走把我拉回来哦，是的资讯科技努力地有时是但是现在我够强壮来告诉悲哀再见仅仅喜欢，月亮将夜晚装满星我将在空气中跳舞黑暗的找寻没有东西害怕，我将会站而且飞离开进入夜晚之内我将找事实痛苦从不结束但是总是有是一个希望在这银河中是自由地永恒地在另外的一个日子居住太阳将会表示方式而且我将会跟随进入商标新的日子之内夜晚之久突破我的旅程继续进入欢喜和事实的光之内哪里总是有伤心事在那里意志是一个希望对于我们能是的一个地方我们想要当谁你的泪滴可能褪色离开但是总是保存信心和活的明天进入商标新的日子之内夜晚之久突破你的被开始的旅程

The carbons in explosives are decomposed into gas-like carbon atoms in the detonation reaction zone, then unoxidized carbon atoms coagulate into carbon liquid droplets and finally consolidate into solid diamonds The new mechanism differs from one conclusion that carbons of explosives are at first transformed into solid graphites, then into diamonds, and also differs from the conclusion that gaseous carbons coagulate into liquid droplets in the reaction zone, then crystalize into diamonds in Taylor rarefaction wave zone.

修正了有关炸药中的碳在反应区内先转变为石墨，然后石墨再发生相变生成金刚石的论点以及认为在反应区内气态碳凝聚为碳液滴，液滴在Taylor稀疏波区凝固成金刚石的论点。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Hey yo jim man why don't ya, you don't you kick some of that you know you know how you do it man it's a trip people don't even believe were together right now but tell your story you know the one i like say it for me riders on the storm riders on the storm into this house we're born (into this house we're born) into this world we're thrown (into this world we're thrown) like a dog without a bone an actor out on loan riders on the storm there's a killer on the road his brain is squirmin' like a toad take a long holiday let your children play if ya give this man a ride sweet family will die killer on the road, yeah goin' off of this off of that with the lizard king bumpin' in the back how bout that driftin', liftin', swiftin', coastin', testaroastin' but the wheels won't stop 200 on the highway fresh up off the block he's a rider, na he's a killer dresses in all black but his hat says stealla pedal to the metal i gotta go hard drive by and say hello hey fredwreck you my mello now let me here what i sound like acapella wow ride dip swish now bring it back just like this like a dog with out his bone unlike a g with out his chrome it's hard to imagine the homey dog in a jag and he's checkin' for the checkered flag comin' in first never in last cause my car to fast i neva eva run out of gas cause i just to clean i do it upper class so fasten your seat belts its so hot it will even make heat melt so get a bowl and roll and ride slip through the slip and slide like a dog without a bone an actor out on loan riders on the storm there's a killer on the road his brain is squirmin' like a toad take a long holiday let your children play if ya give this man a ride sweet family will die killer on the road, yeah need for speed i'm trying to take the lead hold on little homey before you run into the trees i've seen thinks that i would have never saw before hey yo jim let'em in, let'em in open up my back tire smokin' the whole street and now the police wanna flash there lights and chase the dogg all night but i won't pull over nor give up cause i just don't give a fuck yeah from the side boy where we was born and raised straight up to ride boy continuously, we get to an expeditiously keep the light on east side on snoop dogg and the doors and yeah we bout to ride on riders on the storm riders on the storm into this house we're born into this world we're thrown were thrown

嘿摇摇弯轨机人为什么不唉，你不你踢一些那你知道你知道如何你这样做人这是一次旅行人们甚至不相信是在一起此时但告诉你的故事你知道那个我像为我说它骑士在暴风雨上骑士在暴风雨上入这房子我们是出生入这世界我们是扔像个狗没有一根骨头一个演员那儿有一个杀手在路上他的大脑是squirmin'像个癞蛤蟆采取一个长的假期让你的孩子们玩如果唉给这人一个乘甜家将死凶手在路上，是呀去'离开对于这离开关于那和蜥蜴国王 bumpin'在后面如何拳击比赛那 driftin'，liftin'，swiftin'，coastin'，testaroastin'但是这个车轮不会停止两百在公路上新鲜向上远离块他的一个骑士，娜他的一个杀手衣服在纯黑但他的帽子说stealla 踩踏板金属我有去难驶过和问好嘿fredwreck 你我的梅洛现在让我这里什么我听起来像acapella 哇骑现在倾斜豪华带来它回来只是这样像个狗与出来像个狗没有一根骨头一演员借出的骑士在暴风雨上那儿有一个杀手在路上他的大脑是squirmin'像个癞蛤蟆采取一个长的假期让你的孩子们玩如果唉给这人一个乘甜家将死凶手在路上，是呀需要速度我是试图着先鞭继续小像家一样在你未碰撞树木我已经看到想那我从来不锯在以前嘿摇摇弯轨机让'em 在，让'em 在开发我的后面疲劳smokin'一街一巷那么警察要闪光那儿灯光和追逐道格整夜但我不会拦截也不放弃造成我只是不给一个性交是呀自边男孩我们在那里出生和提升直直至骑男孩骑士在暴风雨上骑士在暴风雨上入这房子我们是出生入这世界我们是扔我的翻译不太标准别见怪

ABSTRACT Title of Thesis:A Study of Emergency Medicine System Based on Message Service and Mobile Communication Author:Benson Hung Thesis advised by:Liu, Li Taipei Medical University, Graduate Institute of Medical Informatics This research is based on mobile communication, which will bring all medical information instantly into your hand through carefully built structure and different types of wireless connection. Which can integrate all access information together so it can personalize healthcare information and bring to public the most organized information.

中文摘要论文摘要论文名称：以讯息平台与行动通讯为基础之紧急医疗资讯系统台北医学大学医学资讯研究所研究生姓名：洪培修毕业时间：91 学年度第 2 学期指导教授：刘立台北医学大学医学资讯研究所副教授本研究目的是以讯息服务平台与行动通讯为基础，来提供各项即时的卫生医疗资讯服务，藉由所规划的架构蓝图及各种无线媒体传输的特性，将目前可供应用的资讯存取途径整合在一起，使卫生医疗资源资讯个人化、透明化并受到妥善的规划及运用。

Continuing to deliver and maintain the high standards that we all expect, Akai strides to deeply integrate into everyone's daily life.

AKAI有信心继续保持高水准的服务，更深入地为个人生活打造优良的产品。

We are striving to do better, implementing a process to integrate Alcoa's stringent health and safety standards and culture into

的同时，我们正在实施将美铝公司严格的健康和安全标准及文化融入到新企业的过程。

So when we interact with it, we have to integrate it, to assimilate it into our system.

因此，当我们互动，因此我们一定要结合，吸收到我们的制度。

The boards of such companies should be comprised of elected representatives of the workers in the industry, the wider population, and representatives of a workers' and peasants government to integrate the industry into the overall planning of the economy.

这类公司的董事需要包含行业内工人选出的代表、广大群众产生的代表和作为工农政府的代表从而将整个行业融合到整体的计划经济中。

A carrier gas such as nitrogen is directed through line 20 and valve 22 to connect with line 26 and mix with the gas sample.

如氮气之类的载体通过管线20和阀22引入，与管线26相通，与气体样品混合。

But for the most part, knaves and parasites had the command of his fortune

然而支配他的家产的大多是恶棍和寄生虫。