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In our study,two important lepidopteron pests,beet armyworm and cotton bollworm were surveyed for their endogenous piggyBac.As a result,endogenous piggyBac elements were found in these two insects,and one of the piggyBac elements isolated from the genome of cotton bollworm was intact and thought to be potentially active.1 Clonging and sequence analysis of piggyBac from beet armywormUsing PCR technique,with degenerate primers,a DNA fragment of piggyBac-like element was cloned from the genome of beet armyworm,spodoptera exigua hubner.The DNA fragment was 456bp in length,and the deduced amino acid sequence shares 50%- 78% similarity with other piggyBac elements from insects.But one stop code was found in this DNA fragment.

由于转座子很难用常规的RT-PCR和RACE技术进行克隆鉴定，故本研究在兼并引物PCR的基础上，利用inverse PCR和vectorret PCR技术，不仅调查了鳞翅目两种重要的农业害虫甜菜夜蛾和棉铃虫的內源piggyBac存在情况，同时克隆到了內源性的piggyBac转座子，并且从棉铃虫中获得了一个结构完整、具有潜在活性的piggyBac转座子HaPLE1.1甜菜夜蛾piggyBac转座子基因的克隆与序列分析采用PCR技术，利用兼并引物，从甜菜夜蛾基因组中克隆出一个內源性piggyBac类似因子的DNA片段，并命名为SePLE。

When the animal was intact, it could be made to tremble only when struck by a discharge from a Leyden jar, or when it was no longer living and was a part of a bimetallic circuit.

当动物是完整的时，只发抖可能被做出当从莱顿瓶被一个排出物撞上时，这不再是生活模式，并且是A 一条双金属的电路的部分。

As keeping buildings in a historic block intact is no longer the sole consideration factor, what is more important is the continuity of the cultural background and social linage.

在历史街区里建筑物本体的完整性保存不再是唯一的考量因素，更重要的是整个文化背景与社会脉络的延续。

This knowledge that has given Lindsey the strength to face recent weeks with her composure intact.

这个认识给了Lindsey力量用她的镇静去面对这几周的时间。

Using FLUENT, a large-scale fluid project emulation software, a numerical simulation for cleaning air flow field were made, the intact equivalence line chart and vector graph of the air flow speed distribution were obtained, which provide more overall understanding to the cleaning air flow field speed distribution.

利用大型流体工程仿真软件FLUENT，对清选气流场进行了数值模拟，获得了完整的清选气流速度分布的等值线图和矢量图，对清选气流场的分布有了更全面的了解。

The fatty streak, consisting of lipids and lipoid proteins, located in the intima of the vessel with the overlying endothelium intact, is the earliest form of atherosclerosis.

脂纹在正中，位于完好血管内膜的内皮下，其成分为脂类和脂蛋白，是最早期的动脉粥样硬化。

If, for instance, a person can be treated effectively only by performing a prefrontal lobotomy or by altering in some other more sophisticated fashion, his basic personality or identity, it might well be that punishment would have the virtue of leaving the individual intact.

例如，如果只能通过做前脑叶切除手术或以其他更复杂的方式改变某人的基本的个性或特性才能有效的治疗一个人，那么，惩罚可能更具有消除个人缺陷的美德。

Rat hepatic lobule structure was intact, and liver cells were arranged regularly in the first modeling week.

建模第1周大鼠肝脏的肝小叶结构完整，肝细胞排列整齐。

Held loosely, with respect and freedom for the other person, it is likely to remain intact.

举行宽松地，以另一个人的尊敬和自由，原封保持可能的。

METHODS: Glass ionomer cement was filled into the cavity. The filling surfaces were respectively coated with vaseline, cocoa butter and luminous paint in each group. The control group was left intact.

将玻璃离子体水门汀充填于窝洞中，按照分组分别用凡士林、Coco butter及光亮漆涂于充填体表面，对照组表面不做任何处理。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48