查询词典 insertion method

①Location of WD gene in Ch inese: Using pairwise linkage analysis and multipoint linkage analysis method, w e constructed a genetic map of DNA markers within D13q14.2-3 which refined the location of WD gene by restriction fragment length polymorphism and microsatellite polymorphism analysis;②Screen for mutations of WD gene in Chinese people: we detected the structure of 21 exons of WD ge ne in 45 patients from 39 pedigrees by PCR-SSCP(Single strand conformation poly morphism) and PCR-DNA sequencing technology, found a new mutation in exon 5 and nuclcotide sequence analysis showed it is a T insertion. We also conformed the Arg778Leu in exon 8, the highest frequence mutation point in Chinese people, wit h mutation rate 22.8%in total;③Carrier detection and presymptomatic diagnosi s of WD: Based on DNA recombination technology, we peformed successfully the gen e diagnosis in all individuals of 79 families with WD and built up a helpful spe cific enzyme cut method (PCR-Msp1) to detect the carrier and presympomatic patients in Chinese pe ople with WD.

①WD的基因定位研究：通过RFLP及微卫星多态性分析，应用两位点及多位点连锁软件，建立了中国人WD基因在D13q14.2-3区域的精细遗传连锁图谱，从而首次对中国人WD基因进行了精确定位；②WD基因突变研究：应用PCR-SSCP及DNA测序技术，对39个家系45名WD患者进行该致病基因的21个外显子突变筛选，发现WD基因5号外显子存在新的T插入突变，并证实中国人WD基因的突变热点为8号外显子，突变形式为Arg778Leu，其频率为22.8%；③WD的症状前诊断和杂合子检出：应用DNA重组技术对79个家系进行基因诊断，成功地进行了WD的症状前诊断和杂合子检出，并建立了WD的基因筛选的PCR-Msp 1酶切方法。

The method is just like the QuikChange site-directed mutagenesis method except for its special primer. This pair of primers is composed of a long primer and a short primer which is central overlapped.Both of the Tm of 5\'sequence in long primer and Tm of 3\'sequence in short primer are above 66℃.The Tm of short primer is almost half of that of long primer.It is convenient to perform insertion or substitution mutation of DNA fragments larger than 20bp,In addition,it is highly applicable in research about the deletion mutation of DNA fragments larger than 2000bp.

我们实验室一直在寻找一种简单高效的可以用于基因的删除、插入及替代突变的方法，通过大量研究和实验发现，可以使用一种基于反向PCR原理，使用全新的引物设计的突变方法——&COP&突变法，该方法采用与QuikChange点突变法相同的操作步骤，其策略是设计一对中心重合的引物，长引物的5\'端序列和3\'端序列的Tm值均不低于66℃，短引物Tm值约为长引物一半，且与长引物中心重合互补。

In the light of the complex, high-level and non-linear feature of the mathematical model which describe the transport of the coalbed methane, this paper study the fully-implicit solving method of the mathematical model in detail. Based on the complexity of the algebraic equations which are formed eventually, according to the alternating direction implicit difference pattern, this paper use the iterative method and the fully main element Gauss-Jordan eliminating method to solve equations, which is to use the iterative method to determine coefficient matrix and use the fully main element Gauss-Jordan method to solve th linear algebraic equation group, at the same time of studying the solving method of the mathematical model, according to the devising requirement of FORTRAN77 program structure, this paper draw up computer program and form the corresponding computer model, and verify the validity and reliability of the model in theory by operating the model.

重点研究了模型内、外边界及有关参数的处理，针对描述煤层甲烷运移的数学模型是一个复杂、高阶非线性数学模型的特点，详细研究了模型的全隐式求解方法，根据最后形成的代数方程组的复杂性，按交替方向隐式差分格式，采用迭代与全选主元高斯约当消去法相结合的方法求解方程：即确定系数矩阵采用迭代法，求解线性方程组时采用全选主元高斯约当消去法，在研究模型解法的同时按FORTRAN结构化程序设计的要求，编制计算机程序，形成相应的CBMRS计算机模型，并通过模型的运行从理论上证明了模型的正确性与可靠性。

The description methods of natural scenery was studied based on fractal, that is, recursive method, L-system method, IFS and IFS description method was put forward based on sine or cosine. This method overcomes angularity and consistency of recursive method, L-system method and IFS, and the variety of fractal sculpting consequence can be added and the basis for the later research and the application was provided.

对基于分形的描述自然景物方法，即递归洪、L-系统法、IFS法进行了分析研究，在此基础上，提出基于正余弦的IFS描述法，该方法克服了递归法、L-系统法、IFS法等描述方法的生硬性和一律性，增加了分形造型结果的多样性，为以后的深入研究和应用提供了依据。

The CBRC allows commercial banks to implement the internal rating method by stages, however, when a commercial bank obtains the approval for using the internal rating method, its asset coverage ratio calculated according to the internal rating method (risk-weighted assets calculated according to the internal rating method)/(risk-weighted assets calculated according to the internal rating method + risk-weighted assets of other credit risk exposures calculated according to the revised capital supervisory provisions) shall not be lower than 50%, and it shall formulate the plan for the implementation of internal rating method by stages, so as to ensure that the asset coverage ratio reach 80% in three years.

银监会允许商业银行分阶段实施内部评级法，但在获得许可使用内部评级法时，采用内部评级法的资产覆盖率（按内部评级法计算的风险加权资产/［按内部评级法计算的风险加权资产＋按修订后资本监管规定计算的其他信用风险暴露的风险加权资产］）应不低于50％，并制定分阶段实施内部评级法规划，以保证3年内资产覆盖率达到80％。

In chapter 2,all kinds of numerical methods of computational fracturemechanics are summarized,the development histories and present situation ofboundary collocation method,boundary integral method,finite difference method,boundary element method and finite element method are expounded,and theapplication of finite element method in fracture mechanics are emphaticallydiscussed.

第二章 总结了计算断裂力学中各种数值方法，论述了边界配位法、边界积分法、有限差分法、边界元法及有限元法的发展历史和现状，并着重讨论了有限元法在断裂力学中的应用。

The stress analytic method of arch dam covers pure arch method, arch beam sharing method, finite element method, calculating method of shell theory and structural model test method.

拱坝的应力分析方法包括纯拱法、拱梁分载法、有限元法、壳体理论计算方法和结构模型试验法。

In order to verify the method, the comparison among the Tabary slope method, the Bayes function method, the Yamada continuity method and the background error covariance function method is presented. The results show that the background error covariance function method is the best one.

为检验此法效果，利用1999年5月3日到4日奥克拉荷马强龙卷的雷达资料，给出风廓线仪的实测结果对比分析，Tabary的切线拟合方法和条件概率方法，Yamada的连续性检测方法的对比试验及退模糊后的径向速度图。

In the text, first introduce the constitute, principle and characteristics of the cchp system which is a comprehensive energy system; in the following, overview the domestic development and the research situations of outside in this realm; immediately after, introduce in brief the research object"s engineering general situation and the design projects that be brought up aimed at this engineering in this text; then detailed discuss a kind of lately thermal economics cost absorption method- the method of share the cost of the product"s Exergy according to the ability class, and make use of this method and the product Exergy equivalence method and steam equivalence method to carry economics analysis to the design project respectively,and carry on the economy compared for the calculation of the several kind different cost absorption method from the point of view that the price of the energy Exergy.

文中，首先介绍了热电冷联产这一综合能源系统的组成、原理及特点；综述了国内外在这一领域的发展和研究概况；接着，简单的介绍了本文所研究对象的工程概况及针对本工程所提出的设计方案；然后详细讨论了一种新的热经济学成本分摊方法-按能级分摊产品火用成本法，并分别运用此方法和产品火用等价法、蒸汽等价法对设计方案进行经济性分析，从能量火用价格的角度对几种不同分摊方法的计算结果进行经济性比较。

For transfers of tangible property, a taxpayer is entitled to use (1) the comparable uncontrolled price method;(2) the resale price method;(3) the cost-plus method;(4) the comparable profits method;(5) the profit-split method; or (6) an "unspecified method".

对于转 让有形资产，纳税人有权采用：(1)可比非受控价格法；(2)再销售价格法；(3)成本加成法；(4)可比较利润法(5)利润分离法；或(6)其它方法。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。